2015 Vol. 33, No. 3
Display Method:
2015, 33(3): 193-197.
doi: 10.3969/j.issn.1006-0111.2015.03.001
Abstract:
The research progress on new technologies for high throughput screening of effective traditional Chinese medicine (TCM) components was summarized based on the recent documents at home and abroad, among which bio-chromatography, chip-technology and computer-aided virtual screen technology were widely used. Compared with traditional screening technology, those new ones had shown advantages in efficiency, automation and high-throughput, providing new ways to screen effective components of TCM with high throughput.
The research progress on new technologies for high throughput screening of effective traditional Chinese medicine (TCM) components was summarized based on the recent documents at home and abroad, among which bio-chromatography, chip-technology and computer-aided virtual screen technology were widely used. Compared with traditional screening technology, those new ones had shown advantages in efficiency, automation and high-throughput, providing new ways to screen effective components of TCM with high throughput.
2015, 33(3): 198-200,249.
doi: 10.3969/j.issn.1006-0111.2015.03.002
Abstract:
The chemical constituents of Rosa chinensis Jacq were diverse, mainly including flavonoids, flavonoid glycosides, phenolic acids, aromatic oils, tannins and pigments. Its extract and some chemical constituents had shown multiple pharmacological activities, such as antitumor, antifungal, anti-viral, anti-oxidation etc..The advances in the study on chemical components and pharmacological actions of Rosa chinensis Jacq were reviewed and its application prospect was prospected.
The chemical constituents of Rosa chinensis Jacq were diverse, mainly including flavonoids, flavonoid glycosides, phenolic acids, aromatic oils, tannins and pigments. Its extract and some chemical constituents had shown multiple pharmacological activities, such as antitumor, antifungal, anti-viral, anti-oxidation etc..The advances in the study on chemical components and pharmacological actions of Rosa chinensis Jacq were reviewed and its application prospect was prospected.
2015, 33(3): 201-204,220.
doi: 10.3969/j.issn.1006-0111.2015.03.003
Abstract:
Celastrus orbiculatus Thunb. (Celastraceae) was widely distributed in China and more in the south area of the Yangtze River. Research showed the main chemical components including terpenoids, lipids, glycosides and flavonoids. Its pharmacological effects were extensive, which were including antitumor, anti-inflammatory, immunomodulatory, anti-fibrosis and anti-fertility etc. The separation of active compositions and advances in the pharmacological effects were reviewed in recent years, which would be helpful in providing reference and thinking for the research and development of Celastrus orbiculatus.
Celastrus orbiculatus Thunb. (Celastraceae) was widely distributed in China and more in the south area of the Yangtze River. Research showed the main chemical components including terpenoids, lipids, glycosides and flavonoids. Its pharmacological effects were extensive, which were including antitumor, anti-inflammatory, immunomodulatory, anti-fibrosis and anti-fertility etc. The separation of active compositions and advances in the pharmacological effects were reviewed in recent years, which would be helpful in providing reference and thinking for the research and development of Celastrus orbiculatus.
2015, 33(3): 205-208,216.
doi: 10.3969/j.issn.1006-0111.2015.03.004
Abstract:
The novel drug gel was a new formulation, which was applied more and more in medicine field recently. The intelligent hydrogel, liposome gels, clathrate gels were summarized, which could be provide reference for counterparts.
The novel drug gel was a new formulation, which was applied more and more in medicine field recently. The intelligent hydrogel, liposome gels, clathrate gels were summarized, which could be provide reference for counterparts.
2015, 33(3): 209-212.
doi: 10.3969/j.issn.1006-0111.2015.03.005
Abstract:
Objective To investigate the metabolism in miconazole-treated Candida albicans, search for possible biomarkers and discuss the mechanism of miconazole. Methods GC-MS was employed to determine the metabolic fingerprint of Candida albicans before and after treatment with miconazole, and the difference based on multivariate was compared by statistical analysis, the potential biomarkers were screened out and the mechanism of miconazole was discussed. Results Twenty three metabolites was screened out as potential biomarkers, and they were primarily involved in amino acid metabolism, citrate cycle, glycolysis and lipid metabolism. Conclusion The antifungal activity of miconazole was played by affecting a variety of metabolic pathways.
Objective To investigate the metabolism in miconazole-treated Candida albicans, search for possible biomarkers and discuss the mechanism of miconazole. Methods GC-MS was employed to determine the metabolic fingerprint of Candida albicans before and after treatment with miconazole, and the difference based on multivariate was compared by statistical analysis, the potential biomarkers were screened out and the mechanism of miconazole was discussed. Results Twenty three metabolites was screened out as potential biomarkers, and they were primarily involved in amino acid metabolism, citrate cycle, glycolysis and lipid metabolism. Conclusion The antifungal activity of miconazole was played by affecting a variety of metabolic pathways.
2015, 33(3): 213-216.
doi: 10.3969/j.issn.1006-0111.2015.03.006
Abstract:
Objective To establish a paper-based surface-enhanced Raman scattering (SERS) method for the detection of dyed Kadsura longipedunculata Finet et Gagnep. Methods Supported silver nanoparticles on filter paper were synthesized simply by soaking method.In addition, factors including enrichment ratio of silver nanoparticles, the enhancement effect and stability of the SERS device were investigated. Results The Kadsura longipedunculata Finet et Gagnep, dyed by Erythrosine or Acid Red at low concentration had been detected successfully. Conclusion Combined with the paper device and SERS, the approach was rapid and non-destructive which could be used to identification of dyed Kadsura longipedunculata Finet et Gagnep.
Objective To establish a paper-based surface-enhanced Raman scattering (SERS) method for the detection of dyed Kadsura longipedunculata Finet et Gagnep. Methods Supported silver nanoparticles on filter paper were synthesized simply by soaking method.In addition, factors including enrichment ratio of silver nanoparticles, the enhancement effect and stability of the SERS device were investigated. Results The Kadsura longipedunculata Finet et Gagnep, dyed by Erythrosine or Acid Red at low concentration had been detected successfully. Conclusion Combined with the paper device and SERS, the approach was rapid and non-destructive which could be used to identification of dyed Kadsura longipedunculata Finet et Gagnep.
2015, 33(3): 217-220.
doi: 10.3969/j.issn.1006-0111.2015.03.007
Abstract:
Objective To study and optimize the preparation process and abserve the related properties of electret fluorouracil gel patch. Methods the adhesives and moistening agents were screened by preliminary experiment. The optimal matrix proportion in the blank gel patch was obtained with orthogonal design. The charge storage stability of the patch was studied by compensation method. The transdermal behaviors of the patch were evaluated by modified diffusion cell method and HPLC. Results The optimal matrix proportions of PAAS:gelatin:PVA:Kaolin:CMC-Na:moistening agents were 0.2:0.7:0.5:0.7:0.2:12.The patch had a good charge storage stability. All the electrets and 3% azone could enhance the cumulative penetration amount of drug and they had synergistic enhancing effect. Conclusion The qualified electret fluorouracil gel patch was prepared under the optimal process, which had no skin irritation and the behavior of transdermal delivery was better than that of the traditional gel patch.
Objective To study and optimize the preparation process and abserve the related properties of electret fluorouracil gel patch. Methods the adhesives and moistening agents were screened by preliminary experiment. The optimal matrix proportion in the blank gel patch was obtained with orthogonal design. The charge storage stability of the patch was studied by compensation method. The transdermal behaviors of the patch were evaluated by modified diffusion cell method and HPLC. Results The optimal matrix proportions of PAAS:gelatin:PVA:Kaolin:CMC-Na:moistening agents were 0.2:0.7:0.5:0.7:0.2:12.The patch had a good charge storage stability. All the electrets and 3% azone could enhance the cumulative penetration amount of drug and they had synergistic enhancing effect. Conclusion The qualified electret fluorouracil gel patch was prepared under the optimal process, which had no skin irritation and the behavior of transdermal delivery was better than that of the traditional gel patch.
2015, 33(3): 221-225.
doi: 10.3969/j.issn.1006-0111.2015.03.008
Abstract:
Objective To improve the degree of substitution of the cholesteryl by purifying cholesteryl succinyl ester(CHS), one of the raw materials. Methods CHS was purified by recrystallization, the pure CHS structure was characterizate by differential scanning calorimetry (DSC) and X ray diffraction (XRD), the free cholesterol content of the pure and crude were determinated by high performance liquid chromatography (HPLC), the degree of substitution of the cholesteryl was determinated before and after the purified by 1H-NMR. Result CHS was purer after recrystallization, with property of crystal, which could significantly improve the degree of substitution of the cholesteryl. Conclusion The method of purification was simple and feasible, which was the basis of improving hydrophobically modified Bletilla striata polysaccharide's degree of substitution of the cholesteryl.
Objective To improve the degree of substitution of the cholesteryl by purifying cholesteryl succinyl ester(CHS), one of the raw materials. Methods CHS was purified by recrystallization, the pure CHS structure was characterizate by differential scanning calorimetry (DSC) and X ray diffraction (XRD), the free cholesterol content of the pure and crude were determinated by high performance liquid chromatography (HPLC), the degree of substitution of the cholesteryl was determinated before and after the purified by 1H-NMR. Result CHS was purer after recrystallization, with property of crystal, which could significantly improve the degree of substitution of the cholesteryl. Conclusion The method of purification was simple and feasible, which was the basis of improving hydrophobically modified Bletilla striata polysaccharide's degree of substitution of the cholesteryl.
2015, 33(3): 226-230.
doi: 10.3969/j.issn.1006-0111.2015.03.009
Abstract:
Objective To analyze the different metabolites of the classical activated(M1), alternatively activated(M2) and resting BV2 cells by metabolomics method. Methods The mRNAs of several potential biomarkers were determined by real-time PCR analyses to confirm the state of BV2 cells. Static GC-MS combined with metabolomics technology was used to analyze the metabolic changes. Results There were 15 biomarkers identified between the M1 group and the resting group, and 15 biomarkers were found in the M2 group and the resting group. Conclusion The present study provides an effective way to reveal the mechanism of the polarization of BV2 cell, and it might provide a theoretical basis to prevent or treat the neurodegenerative diseases.
Objective To analyze the different metabolites of the classical activated(M1), alternatively activated(M2) and resting BV2 cells by metabolomics method. Methods The mRNAs of several potential biomarkers were determined by real-time PCR analyses to confirm the state of BV2 cells. Static GC-MS combined with metabolomics technology was used to analyze the metabolic changes. Results There were 15 biomarkers identified between the M1 group and the resting group, and 15 biomarkers were found in the M2 group and the resting group. Conclusion The present study provides an effective way to reveal the mechanism of the polarization of BV2 cell, and it might provide a theoretical basis to prevent or treat the neurodegenerative diseases.
2015, 33(3): 231-234.
doi: 10.3969/j.issn.1006-0111.2015.03.010
Abstract:
Objective To evaluate the safety of fibrin sealant (FS) intraperitoneal injection in SD rats. Methods 80 male and female SD rats were randomly divided into four groups (0, 85.5, 171.0, 342.0 mg/kg) by body weight. All rats were intraperitoneally injected with vehicle or FS daily for 14 days followed by a 28-day recovery period. The clinical signs, hematological and biochemical indices were measured. The pathology were observed. Results Increase of white blood cell count (WBC) and decrease of fibrinogen (FIB) in d 14 were found in 171.0 mg/kg and 342.0 mg/kg dosage groups. Furthermore, the tendency of weight increase of spleen were found in 171.0 mg/kg and 342.0 mg/kg dosage groups. Pathological exams of peritoneal cavity found that there were granulation tissues containing FS in some of the rats in 342.0 mg/kg group. All of these changes got reversed after the recovery period. Conclusion The safety dose in this study is considered to be 85.5 mg/kg, and the toxicity dose is 171.0 mg/kg. The target toxicity systems or site of FS in SD rats are hematological system, immune system and injection site. The toxic effects of FS are reversible.
Objective To evaluate the safety of fibrin sealant (FS) intraperitoneal injection in SD rats. Methods 80 male and female SD rats were randomly divided into four groups (0, 85.5, 171.0, 342.0 mg/kg) by body weight. All rats were intraperitoneally injected with vehicle or FS daily for 14 days followed by a 28-day recovery period. The clinical signs, hematological and biochemical indices were measured. The pathology were observed. Results Increase of white blood cell count (WBC) and decrease of fibrinogen (FIB) in d 14 were found in 171.0 mg/kg and 342.0 mg/kg dosage groups. Furthermore, the tendency of weight increase of spleen were found in 171.0 mg/kg and 342.0 mg/kg dosage groups. Pathological exams of peritoneal cavity found that there were granulation tissues containing FS in some of the rats in 342.0 mg/kg group. All of these changes got reversed after the recovery period. Conclusion The safety dose in this study is considered to be 85.5 mg/kg, and the toxicity dose is 171.0 mg/kg. The target toxicity systems or site of FS in SD rats are hematological system, immune system and injection site. The toxic effects of FS are reversible.
2015, 33(3): 235-237,241.
doi: 10.3969/j.issn.1006-0111.2015.03.011
Abstract:
Objective To screen the hemostatic active extracts from Callicarpa nudiflora. Methods The powdered Callicarpa nudiflora was extracted with 70% EtOH and concentrated to give EtOH-extract. The EtOH-extract was further chromatographed over HP-20 macroporous resin column, eluting with aqua and 95% EtOH to get HP-H2O-elution and HP-EtOH-elution, respectively. The obtained three extracts of EtOH-extrract, HP-EtOH-elution and HP-H2O-elution were set as large, middle and small dosage groups for drug preparation, respectively. Yunnan Baiyao was used as a positive control group.The weight increment, bleeding time and clotting time of fed mice were detected by cutting tail and grass slide Methods after intragastric administration for 7 days. Results As compared with blank model and positive control, each dosage groups of HP-EtOH-elution could significantly shortened the bleeding time, of which the small dosage group and middle dosage group even exhibited better Results than the positive control group.Whereas the EtOH-extract, HP-EtOH-elution and HP-H2O-elution didn't demonstrated significant effect on clotting time as well as the weight increment. Conclusion The HP-EtOH-elution was suggested to be the major active extract possessing hemostatic activity to mice under tested dosages.The hemostatic mechanism may through intrinsic coagulation pathway.This study would be helpful for further phytochemical investigation of C.nudiflora.
Objective To screen the hemostatic active extracts from Callicarpa nudiflora. Methods The powdered Callicarpa nudiflora was extracted with 70% EtOH and concentrated to give EtOH-extract. The EtOH-extract was further chromatographed over HP-20 macroporous resin column, eluting with aqua and 95% EtOH to get HP-H2O-elution and HP-EtOH-elution, respectively. The obtained three extracts of EtOH-extrract, HP-EtOH-elution and HP-H2O-elution were set as large, middle and small dosage groups for drug preparation, respectively. Yunnan Baiyao was used as a positive control group.The weight increment, bleeding time and clotting time of fed mice were detected by cutting tail and grass slide Methods after intragastric administration for 7 days. Results As compared with blank model and positive control, each dosage groups of HP-EtOH-elution could significantly shortened the bleeding time, of which the small dosage group and middle dosage group even exhibited better Results than the positive control group.Whereas the EtOH-extract, HP-EtOH-elution and HP-H2O-elution didn't demonstrated significant effect on clotting time as well as the weight increment. Conclusion The HP-EtOH-elution was suggested to be the major active extract possessing hemostatic activity to mice under tested dosages.The hemostatic mechanism may through intrinsic coagulation pathway.This study would be helpful for further phytochemical investigation of C.nudiflora.
2015, 33(3): 238-241.
doi: 10.3969/j.issn.1006-0111.2015.03.012
Abstract:
Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina. Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry. Taking the content of total iridoid glycosides and total isoflavones as indexes, the effect of the concentration of sample solution, medicinal herbs on the amount of sample, concentration and volume of eluent was investigated by single factor test. Results The optimum separation and purification technology was as following: the concentration of sample solution was 0.1 g/ml, the volume ratio of resin to material drug was 2:1, the adsorption time was 2 h, and the sample was firstly eluted with 1 BV water, then 6 BV 20% ethanol and 60% ethanol, and the eluent was collected. Conclusion This optimized separation and purification technology was reasonable and stable, and it could be extended to large-scale production applications.
Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina. Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry. Taking the content of total iridoid glycosides and total isoflavones as indexes, the effect of the concentration of sample solution, medicinal herbs on the amount of sample, concentration and volume of eluent was investigated by single factor test. Results The optimum separation and purification technology was as following: the concentration of sample solution was 0.1 g/ml, the volume ratio of resin to material drug was 2:1, the adsorption time was 2 h, and the sample was firstly eluted with 1 BV water, then 6 BV 20% ethanol and 60% ethanol, and the eluent was collected. Conclusion This optimized separation and purification technology was reasonable and stable, and it could be extended to large-scale production applications.
2015, 33(3): 242-245,274.
doi: 10.3969/j.issn.1006-0111.2015.03.013
Abstract:
Objective To investigate the chemical constituents of marine sponge Agelas mauritiana collected from the South China Sea. Methods The n-butanol extract of marine sponge Agelas mauritiana was separated and purified by repeated column chromatography on silica gel, Sephadex LH-20, and reversed-phase high-performance liquid chromatography (RP-HPLC). The chemical structures of those obtained compounds were determined on the basis of spectroscopic analysis and comparison with reported data. The tumor cell growth inhibitory activity of these compounds towards human lung carcinoma cells A549 was tested. Results Eight compounds were isolated, including agelasine A ( 1 ), agelasine B ( 2 ), epi-agelasine C ( 3 ), (-)agelasine D ( 4 ), agelasine E ( 5 ), agelasine F ( 6 ), (-)ageloxime D ( 7 ) and aurantiamide acetate ( 8 ). These compounds displayed different level of tumor cell growth inhibitory activity towards cell A549 in vitro. Compounds 1 - 3 showed significant activity towards cell A549, being similar to that of the positive control of adriamycin. Conclusion Compounds 1 , 2 , 4 , 5 , 6 , 8 were isolated for the first time from the sponge Agelas mauritiana. Cell A549 was selected for the first time for the activity evaluation of compounds 1 ~ 7 .Significant inhibition activity of compounds 2 、 3 may hold as a basis for further research.
Objective To investigate the chemical constituents of marine sponge Agelas mauritiana collected from the South China Sea. Methods The n-butanol extract of marine sponge Agelas mauritiana was separated and purified by repeated column chromatography on silica gel, Sephadex LH-20, and reversed-phase high-performance liquid chromatography (RP-HPLC). The chemical structures of those obtained compounds were determined on the basis of spectroscopic analysis and comparison with reported data. The tumor cell growth inhibitory activity of these compounds towards human lung carcinoma cells A549 was tested. Results Eight compounds were isolated, including agelasine A ( 1 ), agelasine B ( 2 ), epi-agelasine C ( 3 ), (-)agelasine D ( 4 ), agelasine E ( 5 ), agelasine F ( 6 ), (-)ageloxime D ( 7 ) and aurantiamide acetate ( 8 ). These compounds displayed different level of tumor cell growth inhibitory activity towards cell A549 in vitro. Compounds 1 - 3 showed significant activity towards cell A549, being similar to that of the positive control of adriamycin. Conclusion Compounds 1 , 2 , 4 , 5 , 6 , 8 were isolated for the first time from the sponge Agelas mauritiana. Cell A549 was selected for the first time for the activity evaluation of compounds 1 ~ 7 .Significant inhibition activity of compounds 2 、 3 may hold as a basis for further research.
2015, 33(3): 246-249.
doi: 10.3969/j.issn.1006-0111.2015.03.014
Abstract:
Objective To analyze and compare the chemical components of the volatile oil in different parts of Cryptotaenia japonica. Methods Essential oils were extracted from the root, stem, leaf, fruit of Cryptotaenia japonica through steam distillation. GC-MS analysis combined with Kovats retention index Methods were used to analyze its main components. Results 28 peaks were separated and 28 compounds were identified from volatile oils from the root of Cryptotaenia japonica, accounting for 86.66% of the total, 27 peaks were separated and 27 compounds were identified from volatile oils from stem of Cryptotaenia japonica, accounting for 88.01% of the total, 27 peaks were separated and 27 compounds were identified from volatile oils from leaves of Cryptotaenia japonica, accounting for 94.03% of the total, 26 peaks were separated and 26 compounds were identified from volatile oils from fruit of Cryptotaenia japonica, accounting for 88.32% of the total. 35 kinds of volatile compounds were identified from the whole strain, and the main components of each part of the volatile oil were sesquiterpene compounds, containing a small amount of monoterpenes. Among all the 35 compounds, different parts of Cryptotaenia japonica share 22 compounds. Compounds 19 , 23 only exists in the volatile oil from the roots. Aromadendrene, compound 25 , red myrrh alcohol only exists in the volatile oil from the fruit. So there is a certain difference in the types and quantity of the compounds. Conclusion The volatile oil of Cryptotaenia japonica contains many physiologically active substance. Take the whole plant as a medicine has its rationality, which provide a scientific basis for the further research and development of Cryptotaenia japonica.
Objective To analyze and compare the chemical components of the volatile oil in different parts of Cryptotaenia japonica. Methods Essential oils were extracted from the root, stem, leaf, fruit of Cryptotaenia japonica through steam distillation. GC-MS analysis combined with Kovats retention index Methods were used to analyze its main components. Results 28 peaks were separated and 28 compounds were identified from volatile oils from the root of Cryptotaenia japonica, accounting for 86.66% of the total, 27 peaks were separated and 27 compounds were identified from volatile oils from stem of Cryptotaenia japonica, accounting for 88.01% of the total, 27 peaks were separated and 27 compounds were identified from volatile oils from leaves of Cryptotaenia japonica, accounting for 94.03% of the total, 26 peaks were separated and 26 compounds were identified from volatile oils from fruit of Cryptotaenia japonica, accounting for 88.32% of the total. 35 kinds of volatile compounds were identified from the whole strain, and the main components of each part of the volatile oil were sesquiterpene compounds, containing a small amount of monoterpenes. Among all the 35 compounds, different parts of Cryptotaenia japonica share 22 compounds. Compounds 19 , 23 only exists in the volatile oil from the roots. Aromadendrene, compound 25 , red myrrh alcohol only exists in the volatile oil from the fruit. So there is a certain difference in the types and quantity of the compounds. Conclusion The volatile oil of Cryptotaenia japonica contains many physiologically active substance. Take the whole plant as a medicine has its rationality, which provide a scientific basis for the further research and development of Cryptotaenia japonica.
2015, 33(3): 250-252.
doi: 10.3969/j.issn.1006-0111.2015.03.015
Abstract:
Objective To establish an HPLC-DAD method for simultaneous determination of four ingredients in Gan Bawei capsule. Methods HPLC method was adopted with C18 column(250 mm ×4.6 mm, 5 μm), Acetonitrile as mobile phase A and water as mobile phase B in gradient elution mode. The detection wavelength was 240 nm and the flow rate was 1.0 ml/min. Results Salvianolic acid B reference substance was excellently linear in the range of 15.026~100.17 μg/ml, the average recovery was 96.6%(n=6)and RSD was 1.8%(n=6). Paeoniflorin reference substance was excellently linear in the range of 14.335~95.564 μg/ml, the average recovery was 97.0%(n=6)and RSD was 1.7%(n=6).Polydatin reference substance was excellently linear in the range of 8.235-54.90 μg/ml, the average recovery was 97.2%(n=6)and RSD was 1.8%(n=6).Emodin reference substance was excellently linear in the range of 1.582 5-10.55 μg/ml, the average recovery was 98.1%(n=6)and RSD was 1.7%(n=6). Conclusion The method was easy, accurate, reproducible, specific and without interference in negative control, which could be used for quality control of Gan Bawei capsule.
Objective To establish an HPLC-DAD method for simultaneous determination of four ingredients in Gan Bawei capsule. Methods HPLC method was adopted with C18 column(250 mm ×4.6 mm, 5 μm), Acetonitrile as mobile phase A and water as mobile phase B in gradient elution mode. The detection wavelength was 240 nm and the flow rate was 1.0 ml/min. Results Salvianolic acid B reference substance was excellently linear in the range of 15.026~100.17 μg/ml, the average recovery was 96.6%(n=6)and RSD was 1.8%(n=6). Paeoniflorin reference substance was excellently linear in the range of 14.335~95.564 μg/ml, the average recovery was 97.0%(n=6)and RSD was 1.7%(n=6).Polydatin reference substance was excellently linear in the range of 8.235-54.90 μg/ml, the average recovery was 97.2%(n=6)and RSD was 1.8%(n=6).Emodin reference substance was excellently linear in the range of 1.582 5-10.55 μg/ml, the average recovery was 98.1%(n=6)and RSD was 1.7%(n=6). Conclusion The method was easy, accurate, reproducible, specific and without interference in negative control, which could be used for quality control of Gan Bawei capsule.
2015, 33(3): 253-254.
doi: 10.3969/j.issn.1006-0111.2015.03.016
Abstract:
Objective To establish and validate a method of sterility test for nitroglycerin ointment and validate this method. Methods Ten nitroglycerin ointments of 1 g were preheated oven to 45 ℃ for sample, and added to conical flask which containing melted span 80, polysorbate 80 sterile mixture and sterile glass beads, and were shake after mixing, the sample fully emulsified by adding to 100 ml 45 ℃ pH 7.0 sterile sodium chloride-peptone water buffer. According to the method of membrane filtration, bacteria and fungus in each membrane with 300 ml pH 7.0 sterile sodium chloride-peptone water buffer flush, the bacteriostatic activity was eliminated. Results By the method validation, nitroglycerin ointment sample group, negative control group were sterile growth, and test group in each filter of the test bacteria compared with control groups were growing well, so the samples had no inhibitory effect or the antimicrobial effect would not take into account. Conclusion Membrane filtration was reliable, which could be used for sterility test for nitroglycerin ointment.
Objective To establish and validate a method of sterility test for nitroglycerin ointment and validate this method. Methods Ten nitroglycerin ointments of 1 g were preheated oven to 45 ℃ for sample, and added to conical flask which containing melted span 80, polysorbate 80 sterile mixture and sterile glass beads, and were shake after mixing, the sample fully emulsified by adding to 100 ml 45 ℃ pH 7.0 sterile sodium chloride-peptone water buffer. According to the method of membrane filtration, bacteria and fungus in each membrane with 300 ml pH 7.0 sterile sodium chloride-peptone water buffer flush, the bacteriostatic activity was eliminated. Results By the method validation, nitroglycerin ointment sample group, negative control group were sterile growth, and test group in each filter of the test bacteria compared with control groups were growing well, so the samples had no inhibitory effect or the antimicrobial effect would not take into account. Conclusion Membrane filtration was reliable, which could be used for sterility test for nitroglycerin ointment.
2015, 33(3): 255-257.
doi: 10.3969/j.issn.1006-0111.2015.03.017
Abstract:
Objective To consummate a method of quality control of peppermint water. Methods Thin layer chromatography was used for the identification of peppermint oil.Gas chromatography was used for the determination of mentholum. Results The identification of peppermint oil was highly specific by TLC.The linear range of mentholum was from 5.0-80.0 μg/ml, r=0.999 9.The average recovery of mentholum was 103.16% and RSD was 1.81%. Conclusion These Methods were easy to operate with accurate results, which washigh sensitivity andgoodrepeatability.This quality standard could effectively control the quality of peppermint water.
Objective To consummate a method of quality control of peppermint water. Methods Thin layer chromatography was used for the identification of peppermint oil.Gas chromatography was used for the determination of mentholum. Results The identification of peppermint oil was highly specific by TLC.The linear range of mentholum was from 5.0-80.0 μg/ml, r=0.999 9.The average recovery of mentholum was 103.16% and RSD was 1.81%. Conclusion These Methods were easy to operate with accurate results, which washigh sensitivity andgoodrepeatability.This quality standard could effectively control the quality of peppermint water.
2015, 33(3): 258-260,268.
doi: 10.3969/j.issn.1006-0111.2015.03.018
Abstract:
Objective To investigate the influence of gemcitabine chemotherapy on levels of regulatory T cells(Tregs) in peripheral blood for patients with pancreatic cancer and provide evidence and reference for improving the efficacy of adoptive immunotherapy. Methods 32 patients were enrolled in this study from January 2012 to October 2014, among whom 16 received gemcitabine chemotherapy combined with adoptive immunotherapy(gemcitabine group), the other 16 patients received adoptive immunotherapy only(control group).The level of Tregs in peripheral blood, side effect and overall survival were observed before and after the therapy. Results The number of Tregs in peripheral blood was significantly decreased after gemcitabine chemotherapy, and it was also lower than that of the control group. The overall survival time of the gemcitabine group was 1.3 mo longer than the control group(10.0 mo vs 8.7 mo). Conclusion Therapeutic regimen of gemcitabine can remarkly deplete Tregs in peripheral blood of patients with pancreatic cancer, effectively regulate tumor immune tolerance, and improve the efficacy of adoptive immunotherapy.
Objective To investigate the influence of gemcitabine chemotherapy on levels of regulatory T cells(Tregs) in peripheral blood for patients with pancreatic cancer and provide evidence and reference for improving the efficacy of adoptive immunotherapy. Methods 32 patients were enrolled in this study from January 2012 to October 2014, among whom 16 received gemcitabine chemotherapy combined with adoptive immunotherapy(gemcitabine group), the other 16 patients received adoptive immunotherapy only(control group).The level of Tregs in peripheral blood, side effect and overall survival were observed before and after the therapy. Results The number of Tregs in peripheral blood was significantly decreased after gemcitabine chemotherapy, and it was also lower than that of the control group. The overall survival time of the gemcitabine group was 1.3 mo longer than the control group(10.0 mo vs 8.7 mo). Conclusion Therapeutic regimen of gemcitabine can remarkly deplete Tregs in peripheral blood of patients with pancreatic cancer, effectively regulate tumor immune tolerance, and improve the efficacy of adoptive immunotherapy.
2015, 33(3): 263-268.
doi: 10.3969/j.issn.1006-0111.2015.03.020
Abstract:
Objective To assess the efficacy and safety of lamotrigine for absence seizures in children and adolescents. Methods Databases of PubMed, the Cochrane Library, EMbase, CENTRAL, VIP, WanFang, CBM and CNKI were electronically searched till August, 2014 for clinical trials on lamotrigine for absence seizures in children and adolescents. All literature were screened by two reviewers independently according to the inclusion and exclusion criteria.The data was extracted, and the methodological quality was assessed. Then, meta-analysis was performed using RevMan 5.2. Results Seven trials were included involving a total of 721 patients. The Results of methodological qualities were two studies rated as A-class, three studies rated as B-class and two studies rated as C-class. Meta-analysis Results showed that the efficacy of lamotrigine monotherapy for absence seizure in children and adolescents was better than placebo, but efficacy of lamotrigine was lower than valproic acid and ethosuximide. The adverse reaction rates of lamotrigine were with no significant difference compared with valproic acid and ethosuximide. Conclusion Lamotrigine monotherapy was effective for absence seizures in children and adolescents and was well tolerated. Lamotrigine was a good choice for patients that are intolerable to valproic acid or ethosuximide.
Objective To assess the efficacy and safety of lamotrigine for absence seizures in children and adolescents. Methods Databases of PubMed, the Cochrane Library, EMbase, CENTRAL, VIP, WanFang, CBM and CNKI were electronically searched till August, 2014 for clinical trials on lamotrigine for absence seizures in children and adolescents. All literature were screened by two reviewers independently according to the inclusion and exclusion criteria.The data was extracted, and the methodological quality was assessed. Then, meta-analysis was performed using RevMan 5.2. Results Seven trials were included involving a total of 721 patients. The Results of methodological qualities were two studies rated as A-class, three studies rated as B-class and two studies rated as C-class. Meta-analysis Results showed that the efficacy of lamotrigine monotherapy for absence seizure in children and adolescents was better than placebo, but efficacy of lamotrigine was lower than valproic acid and ethosuximide. The adverse reaction rates of lamotrigine were with no significant difference compared with valproic acid and ethosuximide. Conclusion Lamotrigine monotherapy was effective for absence seizures in children and adolescents and was well tolerated. Lamotrigine was a good choice for patients that are intolerable to valproic acid or ethosuximide.
2015, 33(3): 269-271.
doi: 10.3969/j.issn.1006-0111.2015.03.021
Abstract:
Objective To observe the effect of Shuxuetong injection in the improvement of heart and lung function on chronic pulmonary heart disease patients with basic treatment. Methods The control group was given low flow oxygen, sensitive antibiotics for infection control, and other treatments such as cough relief, sputum reduction, and antispasmodic treatment. Treatment group was given 6 ml Shuxuetong injection once a day for 14 days as 1 treatment period. Results The total effective rate of treatment group was 93.75%, while that of total control group was 67.39% (P<0.05) from Chi square test(χ2=11.36, P=0.00). All indexes of blood rheology of treatment group significantly improved compared with self control at an earlier time (P<0.05) and control group (P<0.01). Improvement of the index of pulmonary function between two groups was significant difference(P<0.01). Conclusion Shuxuetong injection as an adjuvant treatment would improve microcirculation, reduce inflammation, and improve patient outcomes. Therapy combined with Shuxuetong injection in the treatment of chronic pulmonary heart disease could significantly improve the efficacy, therefore, which would be worth of clinical use.
Objective To observe the effect of Shuxuetong injection in the improvement of heart and lung function on chronic pulmonary heart disease patients with basic treatment. Methods The control group was given low flow oxygen, sensitive antibiotics for infection control, and other treatments such as cough relief, sputum reduction, and antispasmodic treatment. Treatment group was given 6 ml Shuxuetong injection once a day for 14 days as 1 treatment period. Results The total effective rate of treatment group was 93.75%, while that of total control group was 67.39% (P<0.05) from Chi square test(χ2=11.36, P=0.00). All indexes of blood rheology of treatment group significantly improved compared with self control at an earlier time (P<0.05) and control group (P<0.01). Improvement of the index of pulmonary function between two groups was significant difference(P<0.01). Conclusion Shuxuetong injection as an adjuvant treatment would improve microcirculation, reduce inflammation, and improve patient outcomes. Therapy combined with Shuxuetong injection in the treatment of chronic pulmonary heart disease could significantly improve the efficacy, therefore, which would be worth of clinical use.
2015, 33(3): 272-274.
doi: 10.3969/j.issn.1006-0111.2015.03.022
Abstract:
Objective To observe the curative effect of Baidangba cream for hands and feet chap. Methods 104 cases were divided into the treatment group and the control group containing typeⅠ, Ⅱ, Ⅲ according to chapped degree. The treatment group was treated by Baidangba cream, where the control group was treated by urea ointment. After 15 min warm water immersion, chapped areas were applied with ointments and severely parts were bandaged with elastic tape, for 20 days.4 indicators to observe the efficacy of treatment including bleeding disappeared, cutting softening, pain alleviate, heal chap. Results The curative rate in the treatment group and the control group was 94.3%(50/53) and 88.2%(45/51), respectively. The effective rate in the treatment group and the control group was 5.7%(3/53) and 11.8% (6/51), respectively. There was significant difference between the two group (P<0.05). Conclusion The treatment with Baidangba cream for hands and feet chap was of significant curative effect. It was quite good in the value of application.
Objective To observe the curative effect of Baidangba cream for hands and feet chap. Methods 104 cases were divided into the treatment group and the control group containing typeⅠ, Ⅱ, Ⅲ according to chapped degree. The treatment group was treated by Baidangba cream, where the control group was treated by urea ointment. After 15 min warm water immersion, chapped areas were applied with ointments and severely parts were bandaged with elastic tape, for 20 days.4 indicators to observe the efficacy of treatment including bleeding disappeared, cutting softening, pain alleviate, heal chap. Results The curative rate in the treatment group and the control group was 94.3%(50/53) and 88.2%(45/51), respectively. The effective rate in the treatment group and the control group was 5.7%(3/53) and 11.8% (6/51), respectively. There was significant difference between the two group (P<0.05). Conclusion The treatment with Baidangba cream for hands and feet chap was of significant curative effect. It was quite good in the value of application.
2015, 33(3): 275-277,286.
doi: 10.3969/j.issn.1006-0111.2015.03.023
Abstract:
Objective To study the impact of essential medicine system on rational drug use in Community Health Care Center of Chun'an District. Methods Essential drug system in Community Health Care Center of Chun'an District in Zhejiang Province was taken as the intervention group, and before implementation was taken as contrast group, the essential medicin system was analyzed whether it was or not to promote the rational use of drugs by prescription surveys. Results Average number of drugs per encounter was lower from 2.68 to 2.23(P<0.05), average expenditure per encounter was lower from 42.11 yuan to 37.75 yuan(P<0.05), the use of essential medicines was riser from 79.90% to 89.81%(P<0.05), the three indexes had significant difference, the percentage of injections per encounter was lower from 37.87% to 28.50%(P>0.05), the percentage of antibiotics per encounter was lower from 36.52% to 24.46%(P>0.05), the two indexes had no significant difference. Conclusion After execution of essential medicine system in Community Health Care Center of Chun'an District, average number of drugs and average expenditure per encounter were lower and the use of essential medicines was riser, but the percentage of antibiotics and percentage of injections were not been improved, belonged to irrational category, although there had essential medicines manual, but effective promoting measures still was deficient.
Objective To study the impact of essential medicine system on rational drug use in Community Health Care Center of Chun'an District. Methods Essential drug system in Community Health Care Center of Chun'an District in Zhejiang Province was taken as the intervention group, and before implementation was taken as contrast group, the essential medicin system was analyzed whether it was or not to promote the rational use of drugs by prescription surveys. Results Average number of drugs per encounter was lower from 2.68 to 2.23(P<0.05), average expenditure per encounter was lower from 42.11 yuan to 37.75 yuan(P<0.05), the use of essential medicines was riser from 79.90% to 89.81%(P<0.05), the three indexes had significant difference, the percentage of injections per encounter was lower from 37.87% to 28.50%(P>0.05), the percentage of antibiotics per encounter was lower from 36.52% to 24.46%(P>0.05), the two indexes had no significant difference. Conclusion After execution of essential medicine system in Community Health Care Center of Chun'an District, average number of drugs and average expenditure per encounter were lower and the use of essential medicines was riser, but the percentage of antibiotics and percentage of injections were not been improved, belonged to irrational category, although there had essential medicines manual, but effective promoting measures still was deficient.
2015, 33(3): 278-282.
doi: 10.3969/j.issn.1006-0111.2015.03.024
Abstract:
Objective To evaluate the pharmacoeconomics of two different dosing regimens of levofloxacin treating the bacterial infections of lower respiratory tract, and provide the basis for selecting the security, effective and economic treatment programs clinically. Methods The 108 patients with bacterial infections of lower respiratory tract were divided into the intravenous group and sequential group randomly, the intravenous group used levofloxacin for the intravenous infusion and the sequential group firstly for the intravenous infusion and then for the oral levofloxacin capsule, and the total treatment course of two groups were 7-14 days, and the days of drug use of the two groups should be same so as to conduct the minimal cost analysis for the above two programs. Results The total cost per one patient of the intravenous group (1 779.80±304.15 yuan) was higher than the sequential group(1 290.85±173.76 yuan) significantly, treatment unit of each group needed cost 34.89 yuan and 14.52 yuan respectively. Compared with the sequential group, the intravenous group needed spend more 264.3 yuan.The recovery rate of the two groups was 72.22% and 62.96% respectively(P>0.05), the total effective rate of the two groups was 90.74% and 88.89% respectively(P>0.05), the clearance rate of bacteria of the two groups was 86.96% and 84.44% respectively(P>0.05), and the incidence of adverse reactions was 9.26% and 7.41% respectively(P>0.05). Conclusion The sequential therapy of levofloxacin was a security, effective and economic treatment program, compared with the continuous infusion, and had a more cost-effective advantages, which should be popularized from the consideration of the pharmacoeconomics level.
Objective To evaluate the pharmacoeconomics of two different dosing regimens of levofloxacin treating the bacterial infections of lower respiratory tract, and provide the basis for selecting the security, effective and economic treatment programs clinically. Methods The 108 patients with bacterial infections of lower respiratory tract were divided into the intravenous group and sequential group randomly, the intravenous group used levofloxacin for the intravenous infusion and the sequential group firstly for the intravenous infusion and then for the oral levofloxacin capsule, and the total treatment course of two groups were 7-14 days, and the days of drug use of the two groups should be same so as to conduct the minimal cost analysis for the above two programs. Results The total cost per one patient of the intravenous group (1 779.80±304.15 yuan) was higher than the sequential group(1 290.85±173.76 yuan) significantly, treatment unit of each group needed cost 34.89 yuan and 14.52 yuan respectively. Compared with the sequential group, the intravenous group needed spend more 264.3 yuan.The recovery rate of the two groups was 72.22% and 62.96% respectively(P>0.05), the total effective rate of the two groups was 90.74% and 88.89% respectively(P>0.05), the clearance rate of bacteria of the two groups was 86.96% and 84.44% respectively(P>0.05), and the incidence of adverse reactions was 9.26% and 7.41% respectively(P>0.05). Conclusion The sequential therapy of levofloxacin was a security, effective and economic treatment program, compared with the continuous infusion, and had a more cost-effective advantages, which should be popularized from the consideration of the pharmacoeconomics level.
2015, 33(3): 283-286.
doi: 10.3969/j.issn.1006-0111.2015.03.025
Abstract:
Objective Since it is a common problem for medical practice that paper instructions for medicine information are difficult to update and inconvenient to search, this article is to provide convenient service of pharmacy information for clinical practice by developing medicine information search software in our hospital. Methods According to the practical needs of clinical study and pharmacy research, we took appropriate measures to collect data, categorize contents, scan pictures, polish images and extract information for the medicine instructions. Based on the above work, we applied Oracle 10G to establish medicine information database for our hospital and utilized Java to design medicine information search software. Results Our search software includes 1 201 kinds of medicines, taking up 98.85% of the medicines in our catalogue. The users can use four different search Methods (including common name search, commodity name search, fuzzy search and key word search) to gain the written material and photographic information of medicine instructions. Conclusion With the help of the software of Oracle and Java, the newly designed software for medicine information search can provide convenient pharmacy information service for clinical practice.
Objective Since it is a common problem for medical practice that paper instructions for medicine information are difficult to update and inconvenient to search, this article is to provide convenient service of pharmacy information for clinical practice by developing medicine information search software in our hospital. Methods According to the practical needs of clinical study and pharmacy research, we took appropriate measures to collect data, categorize contents, scan pictures, polish images and extract information for the medicine instructions. Based on the above work, we applied Oracle 10G to establish medicine information database for our hospital and utilized Java to design medicine information search software. Results Our search software includes 1 201 kinds of medicines, taking up 98.85% of the medicines in our catalogue. The users can use four different search Methods (including common name search, commodity name search, fuzzy search and key word search) to gain the written material and photographic information of medicine instructions. Conclusion With the help of the software of Oracle and Java, the newly designed software for medicine information search can provide convenient pharmacy information service for clinical practice.
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