2007 Vol. 25, No. 4
Display Method:
2007, (4): 210-214,234.
Abstract:
Using comparative molecular field analysis（CoMFA）and comparative molecular similarity indices analysis（CoM- SIA）,three dimensional structure-activity relationship（3D-QSAR）has been studied on a series of novel tetralin antifungal com- pounds.Variation of grid spacing was used during the optimization of the CoMFA model.For the CoMSIA study,the influence of the combination of different field types was evaluated and the best combination was considered to be of steric,electrostatic,hydrophobic and H-bonding acceptor fields.Variation of grid spacing and attenuation factor was used to get the best CoMSIA model.The resulting CoMFA and CoMSIA models had a cross validated coeffiecient q2 of 0.618 and 0.613 respectively,which showed strong predictive abil- ity on both test set and training set.The 3D contour maps of CoMFA and CoMSIA provided smooth and interpretable explanation of the structure antifungal activity relationship for the compounds,which will guide the design of novel antifungal compounds with relatively high activity.
Using comparative molecular field analysis（CoMFA）and comparative molecular similarity indices analysis（CoM- SIA）,three dimensional structure-activity relationship（3D-QSAR）has been studied on a series of novel tetralin antifungal com- pounds.Variation of grid spacing was used during the optimization of the CoMFA model.For the CoMSIA study,the influence of the combination of different field types was evaluated and the best combination was considered to be of steric,electrostatic,hydrophobic and H-bonding acceptor fields.Variation of grid spacing and attenuation factor was used to get the best CoMSIA model.The resulting CoMFA and CoMSIA models had a cross validated coeffiecient q2 of 0.618 and 0.613 respectively,which showed strong predictive abil- ity on both test set and training set.The 3D contour maps of CoMFA and CoMSIA provided smooth and interpretable explanation of the structure antifungal activity relationship for the compounds,which will guide the design of novel antifungal compounds with relatively high activity.
2007, (4): 215-218.
Abstract:
Objective: To study the technological parameters of separating total flavornoid and saponin of Tongqiao Biyan tablet with macroporous resin 1300. Methods: With the elution quantity as index,the absorption characteristics and elution parameters were stud- ied. Results: 160 mL extractive of Tongqiao Biyan tablet(crude drugs 0.5g/mL)was added on a column of macroporous resin(φ2.5 cm×H75cm,dry weight 45g)and was washed with 2 BV of distilled water,3 BV of 30% ethanol and 4 BV of 70% ethanol,succes- sively.Total flavonoid and saponin were enriched in the 30% and 70% ethanol elution. Conclusion: This method is simple and feasi- ble with good effect of separateion,which can meet industrial requirements.
Objective: To study the technological parameters of separating total flavornoid and saponin of Tongqiao Biyan tablet with macroporous resin 1300. Methods: With the elution quantity as index,the absorption characteristics and elution parameters were stud- ied. Results: 160 mL extractive of Tongqiao Biyan tablet(crude drugs 0.5g/mL)was added on a column of macroporous resin(φ2.5 cm×H75cm,dry weight 45g)and was washed with 2 BV of distilled water,3 BV of 30% ethanol and 4 BV of 70% ethanol,succes- sively.Total flavonoid and saponin were enriched in the 30% and 70% ethanol elution. Conclusion: This method is simple and feasi- ble with good effect of separateion,which can meet industrial requirements.
2007, (4): 219-221,237.
Abstract:
Objective: To establish a method for quality control of Kangshen pills. Methods: The qualitative analysis of Radix As- tragali,mycelium powder of Cordyceps Sinrnsis,Radix Saposhnikoviae in Kangshen pills were identified by TLC.The content of astra- galosideⅣwas determinated by HPLC. Results: Methods of the qualitative identification were specific and reproducible.The average recovery was 99.39% and relative standard deviation was 0.59%. Conclusion: The precision and reproducibility of the method were good.The method can be used for quality control of Kangshen pills.
Objective: To establish a method for quality control of Kangshen pills. Methods: The qualitative analysis of Radix As- tragali,mycelium powder of Cordyceps Sinrnsis,Radix Saposhnikoviae in Kangshen pills were identified by TLC.The content of astra- galosideⅣwas determinated by HPLC. Results: Methods of the qualitative identification were specific and reproducible.The average recovery was 99.39% and relative standard deviation was 0.59%. Conclusion: The precision and reproducibility of the method were good.The method can be used for quality control of Kangshen pills.
2007, (4): 222-223,260.
Abstract:
Objective: To prepare flurbiprofen liposomal gel and to establish its quality control method. Methods: The liposome was prepared by ethanol injection method and the liposomal gel was prepared using mixing method.The content of flurbiprofen was de termined by HPLC.The entrapment efficiency of flurbiprofen liposome was determined by centrifugation. Results: The average recovery of flurbiprofen was(98.62±2.22)%.The entrapment efficiency of the liposome was(58.44±3.05)%.The pH vaule of the liposo- mal gel was between 6.0 and 7.0.The stability of the liposomal gel was good. Conclusion: This gel is feasible in preparation tech- nique,stable in quality,convenient and reliable in quality control method.
Objective: To prepare flurbiprofen liposomal gel and to establish its quality control method. Methods: The liposome was prepared by ethanol injection method and the liposomal gel was prepared using mixing method.The content of flurbiprofen was de termined by HPLC.The entrapment efficiency of flurbiprofen liposome was determined by centrifugation. Results: The average recovery of flurbiprofen was(98.62±2.22)%.The entrapment efficiency of the liposome was(58.44±3.05)%.The pH vaule of the liposo- mal gel was between 6.0 and 7.0.The stability of the liposomal gel was good. Conclusion: This gel is feasible in preparation tech- nique,stable in quality,convenient and reliable in quality control method.
2007, (4): 224-226.
Abstract:
Objective: To establish a quality control method for Changtai capsules. Methods: Qualitative analysis of Hedyotis difusa Willd and Herba Sarcandrae were carried out by TLC,Radix Pulsatillae was tested by UV.Ethanol-soluble extractive of Changtai cap- sule was determined by Chinese Pharmacopoeia. Results: The extracting solution of the Radix Pulsatillae and it's masculine comparison have the biggest absorption in(201±1)nm,and have the same UV -spectrum.The extractive of Hedyotis difusa Willd and Herba Sar- candrae were assayed by TLC.The results were consistent with the controles.The average content of ethanol-soluble extractive of the samples was 38.8 mg/g.The weight variation,water content,disintegration time,microbiological timiting,test conformed with Chinese pharmacopoeia. Conclusion: This method is simple,accurate,and can be used as the quality control of Changtai capsules.
Objective: To establish a quality control method for Changtai capsules. Methods: Qualitative analysis of Hedyotis difusa Willd and Herba Sarcandrae were carried out by TLC,Radix Pulsatillae was tested by UV.Ethanol-soluble extractive of Changtai cap- sule was determined by Chinese Pharmacopoeia. Results: The extracting solution of the Radix Pulsatillae and it's masculine comparison have the biggest absorption in(201±1)nm,and have the same UV -spectrum.The extractive of Hedyotis difusa Willd and Herba Sar- candrae were assayed by TLC.The results were consistent with the controles.The average content of ethanol-soluble extractive of the samples was 38.8 mg/g.The weight variation,water content,disintegration time,microbiological timiting,test conformed with Chinese pharmacopoeia. Conclusion: This method is simple,accurate,and can be used as the quality control of Changtai capsules.
2007, (4): 227-228.
Abstract:
Objective: To observe curative effect of small dose of ferrous sulfate on pregnant woman with iron deficiency.Method: 106 pregnant women were divided into treatment group and control group randomly.53 patients were in each group.Ferrous sulfate 5 mg/(kg.d)and ferrous sulfate 30 mg/(kg.d)were given to the patients in treatment group and control group. Results: The difference of effective rate between two groups has no significance(P>0.05).The average curative time was(46.3±9.6)d in treatment group, (44.1±8.7)d in control group,which have no significant difference(P>0.05).Coulusion:The small dose of ferrous sulfate had satified curative effect with little side effect and low price,which can be recommended to the grass-root hospital.
Objective: To observe curative effect of small dose of ferrous sulfate on pregnant woman with iron deficiency.Method: 106 pregnant women were divided into treatment group and control group randomly.53 patients were in each group.Ferrous sulfate 5 mg/(kg.d)and ferrous sulfate 30 mg/(kg.d)were given to the patients in treatment group and control group. Results: The difference of effective rate between two groups has no significance(P>0.05).The average curative time was(46.3±9.6)d in treatment group, (44.1±8.7)d in control group,which have no significant difference(P>0.05).Coulusion:The small dose of ferrous sulfate had satified curative effect with little side effect and low price,which can be recommended to the grass-root hospital.
2007, (4): 229-231.
Abstract:
Objective: To investigate the actualities of the distribution and resistance of pathogen isolated from clinical specimens, to provide the basis for clinical rational use drug. Methods: The pathogenes isolated from clinical specimens in 2005 were analysed.Re- sults:It was 1572 pathogenes isolated from clinical specimens which were sent to be examined.Gram positive bacteria was 599,38.1 percent;Gram negative bacteria was 478,30.4 percent;Fungi was 494,31.4 percent;The result of drug sensitive test indicated:the highest sensitive antibiotic:vancomycin 95.8 percent,imipenem 88.2 percent,cefepime 83.6 percent.The highest resistance antibiot- ic:clarithromycin 79.3percent,clindamycin 74.0 percent,erythromycin 73.5 percent. Conclusion: The Fungi infected rate is increas- ing,the rate of Gram positive bacteria and its resistance were high;the enzme-inhibited can reduce the resistance rate of Gram negative bacteria,not reduce the resistance rate of Gram positive bacteria.It would be strengthed the antibiotic rational use in clinic.
Objective: To investigate the actualities of the distribution and resistance of pathogen isolated from clinical specimens, to provide the basis for clinical rational use drug. Methods: The pathogenes isolated from clinical specimens in 2005 were analysed.Re- sults:It was 1572 pathogenes isolated from clinical specimens which were sent to be examined.Gram positive bacteria was 599,38.1 percent;Gram negative bacteria was 478,30.4 percent;Fungi was 494,31.4 percent;The result of drug sensitive test indicated:the highest sensitive antibiotic:vancomycin 95.8 percent,imipenem 88.2 percent,cefepime 83.6 percent.The highest resistance antibiot- ic:clarithromycin 79.3percent,clindamycin 74.0 percent,erythromycin 73.5 percent. Conclusion: The Fungi infected rate is increas- ing,the rate of Gram positive bacteria and its resistance were high;the enzme-inhibited can reduce the resistance rate of Gram negative bacteria,not reduce the resistance rate of Gram positive bacteria.It would be strengthed the antibiotic rational use in clinic.
2007, (4): 232-234.
Abstract:
Objective: To establishe a HPLC method for determination of tolperisone hydrochloride tablets. Methods: A Phenome- nex Luna C~(18) column(250 mm×4.6 mm,5μm)was used,the mobile phases was consisted of acetonitrile-0.1 mol/L sodium acetate (25:75)(adjust pH to 3.5 with acetic acid glacial),the flow rate was 1.2mL/min,the detector wavelength was at 261 nm。 Results: The linear range was 20.2～202.0μg,/mL(r=0.99999),the mean average recovery was 99.9% and RSD was 0.6%。 Conclusion: The method is simple,rapid and accurate.It can be used to quality control the tolperisone hydrochloride tablets.
Objective: To establishe a HPLC method for determination of tolperisone hydrochloride tablets. Methods: A Phenome- nex Luna C~(18) column(250 mm×4.6 mm,5μm)was used,the mobile phases was consisted of acetonitrile-0.1 mol/L sodium acetate (25:75)(adjust pH to 3.5 with acetic acid glacial),the flow rate was 1.2mL/min,the detector wavelength was at 261 nm。 Results: The linear range was 20.2～202.0μg,/mL(r=0.99999),the mean average recovery was 99.9% and RSD was 0.6%。 Conclusion: The method is simple,rapid and accurate.It can be used to quality control the tolperisone hydrochloride tablets.
2007, (4): 235-237.
Abstract:
Objective: To determine residual di-paraxylylene in parylene film coated rubber closure by HPLC. Methods: Parylene film coated rubber closures were extracted with acetone,which then evaporated and transferred with ethanol to volumetric flask,the val- idation of the method was tested and the test condition was confirmed.The chromatography condition was with HP Hyporsil ODS C18 column（4.0 mm×125 mm,5μm）,mobile phase was methanol:water（87:13）,flow speed was 1.0 mL/min,detection wavelength was 224 nm. Results: The peak area of di-paraxylylene correlated well to injection mass in range of 0.1～2.0 mg/L.The stability （RSD）was 0.6% in 24 h.The recoveries were 101.14%（RSD=0.8%,n=9）.The limit of detection was 4.0×10-10g.Conclu- sion:The method was fast and accurate,and suitable for determining the content of residual di-paraxylylene in parylene film coated rubber closure.
Objective: To determine residual di-paraxylylene in parylene film coated rubber closure by HPLC. Methods: Parylene film coated rubber closures were extracted with acetone,which then evaporated and transferred with ethanol to volumetric flask,the val- idation of the method was tested and the test condition was confirmed.The chromatography condition was with HP Hyporsil ODS C18 column（4.0 mm×125 mm,5μm）,mobile phase was methanol:water（87:13）,flow speed was 1.0 mL/min,detection wavelength was 224 nm. Results: The peak area of di-paraxylylene correlated well to injection mass in range of 0.1～2.0 mg/L.The stability （RSD）was 0.6% in 24 h.The recoveries were 101.14%（RSD=0.8%,n=9）.The limit of detection was 4.0×10-10g.Conclu- sion:The method was fast and accurate,and suitable for determining the content of residual di-paraxylylene in parylene film coated rubber closure.
2007, (4): 238-240.
Abstract:
Objective: To establish an accurate and rapid method for determination of ropivacaine by RP-HPLC. Methods: The sample was treated by the addition of bupivacaine as an internal standard,extraction with aether and n-hexane,and dryed by N2.The residue dissolved in the mobile phase and injected into chromatographic system.The HPLC system consisted of AgilentC18 column（4.6 mm×150 mm,5μm）and mobile phase of 0.045 mol/L KH2PO4（pH3.0）-methol（55:45）.Velocity of flow was 1.0 mL/min.The detection wavelength was 210 nm.Column temperature was 30℃. Results: The linear range was from 0.2509μg/mL to 5.018μg/mL with r of 0.999 6,the average recovery was 102.0%.RSD of Intra-day and Inter-day<15%.The results were accord with methodology. Conclusion: This method is quick and stable for ropivacaine monitoring in clinical research.
Objective: To establish an accurate and rapid method for determination of ropivacaine by RP-HPLC. Methods: The sample was treated by the addition of bupivacaine as an internal standard,extraction with aether and n-hexane,and dryed by N2.The residue dissolved in the mobile phase and injected into chromatographic system.The HPLC system consisted of AgilentC18 column（4.6 mm×150 mm,5μm）and mobile phase of 0.045 mol/L KH2PO4（pH3.0）-methol（55:45）.Velocity of flow was 1.0 mL/min.The detection wavelength was 210 nm.Column temperature was 30℃. Results: The linear range was from 0.2509μg/mL to 5.018μg/mL with r of 0.999 6,the average recovery was 102.0%.RSD of Intra-day and Inter-day<15%.The results were accord with methodology. Conclusion: This method is quick and stable for ropivacaine monitoring in clinical research.
Analyses of 4491 valid cases report about monitoring system of the adverse drug reaction in Shanghai
2007, (4): 247-260.
Abstract:
Objective: To analyse of Shanghai adverse drug reaction(ADR)reports,in recent years. Methods: 4491 valid cases reported by the ADR monitoring center network reporting system in Shanghai were analysed. Results: The occurrence of the ADR showed no difference in gender or age.The administration routes were mainly by venous or by mouth(accounting for 87.98%).The ADR referred to 14 drug categories,of which antibiotics took the first place.The ADR involved 25 systems or organs,of which the damage of skin and its appendant organs were primary(accounting for 46.25%). Conclusion: The report and monitoring of the ADR should be paid attention to.
Objective: To analyse of Shanghai adverse drug reaction(ADR)reports,in recent years. Methods: 4491 valid cases reported by the ADR monitoring center network reporting system in Shanghai were analysed. Results: The occurrence of the ADR showed no difference in gender or age.The administration routes were mainly by venous or by mouth(accounting for 87.98%).The ADR referred to 14 drug categories,of which antibiotics took the first place.The ADR involved 25 systems or organs,of which the damage of skin and its appendant organs were primary(accounting for 46.25%). Conclusion: The report and monitoring of the ADR should be paid attention to.
2007, (4): 261-262.
Abstract:
Objective: To investigate 323 reports of adverse drug reactions(ADRs)in our hospital in 2005,and to analyze the re- lationship between ADRs and age,the used drugs. Methods: The cases of ADR were analyzed by descriptive study. Results: 194 kinds of drugs were involved in.Among them,49(25.30%)kinds were antibiotis in the first rank,40(20.60%)kinds were antineo- plastic agents in second rank.ADR showed the lesion of skin and digestive system mostly. Conclusion: Great attention should be paid to the development of ADR so as to reduce the incidence of ADR.
Objective: To investigate 323 reports of adverse drug reactions(ADRs)in our hospital in 2005,and to analyze the re- lationship between ADRs and age,the used drugs. Methods: The cases of ADR were analyzed by descriptive study. Results: 194 kinds of drugs were involved in.Among them,49(25.30%)kinds were antibiotis in the first rank,40(20.60%)kinds were antineo- plastic agents in second rank.ADR showed the lesion of skin and digestive system mostly. Conclusion: Great attention should be paid to the development of ADR so as to reduce the incidence of ADR.
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