2001 Vol. 19, No. 3
Display Method:
2001, (3): 143-145.
Abstract:
OBJECTIVE To develop recombinant human epidermal growth factor (rhEGF) gels for external application to wound healing.METHODS Carbopol was used as water soluable matrix to prepare rhEGF gels.The wound healing effects of rhEGF were evaluated in acute traumatic rats and scalded rats.RESULTS rhEGF gels could be manuf actured easily w it h reliable quality.They could also be applied conveniently to accelerate wound healing in acute traumatic rats and scalded rats.CONCLUSION Such rhEGF gels could be developed for the clinical application.
OBJECTIVE To develop recombinant human epidermal growth factor (rhEGF) gels for external application to wound healing.METHODS Carbopol was used as water soluable matrix to prepare rhEGF gels.The wound healing effects of rhEGF were evaluated in acute traumatic rats and scalded rats.RESULTS rhEGF gels could be manuf actured easily w it h reliable quality.They could also be applied conveniently to accelerate wound healing in acute traumatic rats and scalded rats.CONCLUSION Such rhEGF gels could be developed for the clinical application.
2001, (3): 146-148.
Abstract:
OBJECTIVE To evaluate the bioequivalence assessment of theophylline sustained release capsules in healthy volunteers.METHODSA reversed phase high performance liquid chromatography (RP-HPLC) method was est ablished for the determination of theophylline in plasma after a single oral dose of 200mg domestic theophyline sustained-release capsules and imported theophylline sustained-release capsules in a randomized crossover design.The detector was operated at UV 273nm.RESULTS The dynamic course of theophylline plasma concent rations conformed to a one-compartment open model with a 3P87 program and the main pharmacokinetic parameters after a single oral dose of 200mg domestic sustained-release capsules and imported sust ained-re-lease capsules were as folows Cmax were(5.12±0.74)mg/L and(5.14±0.63)mg/L,tmax were(5.4±1.0)h and(5.3±1.1)h,MRT were(17.79±1.48)h and(17.61±1.78)h,t1/2 were(10.55±0.75)h and(10.59±1.10)h,AUC0-36 were(85.33±10.56)mg·h/L and(86.26±8.80)mg·h/L,respectively.CONCLUSIONS The relative bioavailability of domestic theophylline sustained-release capsules is(98.9±6.4)%.The results of statistical analysis for Cmax,AUC0-36 and AUC0-∞ showed that domestic sustained-release capsules and imported sustained-release capsules were bioequivalent.
OBJECTIVE To evaluate the bioequivalence assessment of theophylline sustained release capsules in healthy volunteers.METHODSA reversed phase high performance liquid chromatography (RP-HPLC) method was est ablished for the determination of theophylline in plasma after a single oral dose of 200mg domestic theophyline sustained-release capsules and imported theophylline sustained-release capsules in a randomized crossover design.The detector was operated at UV 273nm.RESULTS The dynamic course of theophylline plasma concent rations conformed to a one-compartment open model with a 3P87 program and the main pharmacokinetic parameters after a single oral dose of 200mg domestic sustained-release capsules and imported sust ained-re-lease capsules were as folows Cmax were(5.12±0.74)mg/L and(5.14±0.63)mg/L,tmax were(5.4±1.0)h and(5.3±1.1)h,MRT were(17.79±1.48)h and(17.61±1.78)h,t1/2 were(10.55±0.75)h and(10.59±1.10)h,AUC0-36 were(85.33±10.56)mg·h/L and(86.26±8.80)mg·h/L,respectively.CONCLUSIONS The relative bioavailability of domestic theophylline sustained-release capsules is(98.9±6.4)%.The results of statistical analysis for Cmax,AUC0-36 and AUC0-∞ showed that domestic sustained-release capsules and imported sustained-release capsules were bioequivalent.
2001, (3): 149-150.
Abstract:
OBJECTIVE To Produce gentamycin sulfate sustained-release tablets. METHODS With HPMC etc as supplement, gentamycin sulfate sustained-release tablets can be manufactured by way of wet pellets.RESULTS The release limits of the medicine at 2,4 and 6h were above 45%～70%,60%～85% and 80% respectively.CONCLUSION Gentamy cin sulfate sustained-release tablets in acid solvent displays remarkable sustained-release capability.
OBJECTIVE To Produce gentamycin sulfate sustained-release tablets. METHODS With HPMC etc as supplement, gentamycin sulfate sustained-release tablets can be manufactured by way of wet pellets.RESULTS The release limits of the medicine at 2,4 and 6h were above 45%～70%,60%～85% and 80% respectively.CONCLUSION Gentamy cin sulfate sustained-release tablets in acid solvent displays remarkable sustained-release capability.
2001, (3): 151-152,192.
Abstract:
OBJECTIVE To study the stability of lidocaine hydrochloride after alkalinzing.METHODS Lidocaine hydrochloride injection was diluted 4 kinds of concentration.Each sample was added 5% NaHCO3 with different amounts.The pH and concentration of lidocaine were determined in those special alkaline environment in different time.RESULTS The pH of 4 kinds of alkaline lidocaine were remarkably higher than before with theincrease of 5% NaHCO3.The concentration of lidocaine were remarkably increased by time.But the more 5%NaHCO3 added to,the less the concentration of lidocaine was notability.There was some needle-crystal in the solution after 24 hours.CONCLUSION The best amount of 5% NaHCO3 to add in is between 0.5ml to 2.0ml,and lidocaine hydrochloride injection couldnt put by for long time after alkalized.
OBJECTIVE To study the stability of lidocaine hydrochloride after alkalinzing.METHODS Lidocaine hydrochloride injection was diluted 4 kinds of concentration.Each sample was added 5% NaHCO3 with different amounts.The pH and concentration of lidocaine were determined in those special alkaline environment in different time.RESULTS The pH of 4 kinds of alkaline lidocaine were remarkably higher than before with theincrease of 5% NaHCO3.The concentration of lidocaine were remarkably increased by time.But the more 5%NaHCO3 added to,the less the concentration of lidocaine was notability.There was some needle-crystal in the solution after 24 hours.CONCLUSION The best amount of 5% NaHCO3 to add in is between 0.5ml to 2.0ml,and lidocaine hydrochloride injection couldnt put by for long time after alkalized.
2001, (3): 153-153,165.
Abstract:
OBJECTIVE To determinate the kinetics parameter of decompose reaction of clotrimazole.METHODS DTA.was Used.RESULTS The Energy and Progression of decompose reaction of clotrimazole are 131.0kj·mol-1 and 1.029 respectively.CONCLUTION DTA is a simple and easy method to determinate the kinetics paramet er of decompose reaction.
OBJECTIVE To determinate the kinetics parameter of decompose reaction of clotrimazole.METHODS DTA.was Used.RESULTS The Energy and Progression of decompose reaction of clotrimazole are 131.0kj·mol-1 and 1.029 respectively.CONCLUTION DTA is a simple and easy method to determinate the kinetics paramet er of decompose reaction.
2001, (3): 157-159.
Abstract:
OBJECTIVE To investigate the protective mechanism of YOL on ischemia/reperfusion (I/R) myocardium.METHODS48 male SD rats,weighting (260±30)g,were divided into 6 groups.They were normal control,I/R,classical ischemic precondition(C-IPC),sham-operated,Isosorbide dinitrate and YOL.The intracelular calcium concentration of the cardiomyocyte and hemodynamic changes were measured in all groups.RESULTS LVSP、±dp/dt in C-IPC and YOL tread with ischemia 30 min and followed by reperfusion 60 min were obviously higher in I/R(P<0.05)while LVSP、±dp/dt of C-IPC was alsodefinitely high compared with YOL(P<0.05).The intracel ular calcium concentrat ion in C-IPC and YOL were lower in I/R(P<0.05),while[Ca2+]I of YOL was increased in C-IPC (P<0.05).CONCLUSIONS Yix in oral Liquid(YOL)precondit ioning can protect the myocardium from I/R.The possible protective mechanism may be due to decrease calcium overload in cardiomyocyte.
OBJECTIVE To investigate the protective mechanism of YOL on ischemia/reperfusion (I/R) myocardium.METHODS48 male SD rats,weighting (260±30)g,were divided into 6 groups.They were normal control,I/R,classical ischemic precondition(C-IPC),sham-operated,Isosorbide dinitrate and YOL.The intracelular calcium concentration of the cardiomyocyte and hemodynamic changes were measured in all groups.RESULTS LVSP、±dp/dt in C-IPC and YOL tread with ischemia 30 min and followed by reperfusion 60 min were obviously higher in I/R(P<0.05)while LVSP、±dp/dt of C-IPC was alsodefinitely high compared with YOL(P<0.05).The intracel ular calcium concentrat ion in C-IPC and YOL were lower in I/R(P<0.05),while[Ca2+]I of YOL was increased in C-IPC (P<0.05).CONCLUSIONS Yix in oral Liquid(YOL)precondit ioning can protect the myocardium from I/R.The possible protective mechanism may be due to decrease calcium overload in cardiomyocyte.
2001, (3): 160-162.
Abstract:
OBJECTIVE To study the effects of San-he-wei-yao on gastric ulcer in mice and rats. METHODS The models of gastric ulcer were induced by restraint plus water-immersion,reserpine in mice and by pylorus ligation and acetic acid in rats respectively.San-he-wei-yao was given before or after gastric ulcer induction.RESULTS San-he-wei-yao inhibited dose-dependently development of the ulcers.The ulcer size,gastricacid production and pepsin activity were signif icantly decreased by both San-he-wei-yao and cimet idine.CONCLUSION San-he-wei-yao possesses ideal effects on gastric ulcer models.
OBJECTIVE To study the effects of San-he-wei-yao on gastric ulcer in mice and rats. METHODS The models of gastric ulcer were induced by restraint plus water-immersion,reserpine in mice and by pylorus ligation and acetic acid in rats respectively.San-he-wei-yao was given before or after gastric ulcer induction.RESULTS San-he-wei-yao inhibited dose-dependently development of the ulcers.The ulcer size,gastricacid production and pepsin activity were signif icantly decreased by both San-he-wei-yao and cimet idine.CONCLUSION San-he-wei-yao possesses ideal effects on gastric ulcer models.
2001, (3): 163-165,153.
Abstract:
OBJECTIVE Establish a random amplified polymorphism DNA(RAPD) method for classification of origin fungi of the traditional Chinese medicine, Hongqu. METHODS The genome DNAs of the origin fungi of Hongqu from 14 different producing areas were extracted by CTAB. Amplification products were detected by agarose gel elect rophoresis and done clustering analysis using PHYLIP3.5c.RESULTS A characteristic pattern was produced dependent on one of the 10 screened random primers and the genome amplified.The diversities of the fingerprint patterns of Monascus exist obvious by which we can distinguish easily.CONCLUSION RAPD as-say technique is a useful tool to identify of Hongqu.
OBJECTIVE Establish a random amplified polymorphism DNA(RAPD) method for classification of origin fungi of the traditional Chinese medicine, Hongqu. METHODS The genome DNAs of the origin fungi of Hongqu from 14 different producing areas were extracted by CTAB. Amplification products were detected by agarose gel elect rophoresis and done clustering analysis using PHYLIP3.5c.RESULTS A characteristic pattern was produced dependent on one of the 10 screened random primers and the genome amplified.The diversities of the fingerprint patterns of Monascus exist obvious by which we can distinguish easily.CONCLUSION RAPD as-say technique is a useful tool to identify of Hongqu.
2001, (3): 166-167.
Abstract:
OBJECTIVE To establish a method of thin layer chromatographic scanning to determine the content of ferulic acid in Xiao-yao-wan.METHODS Silica G thin layer was used,and the mobile phase consisted of benzene-chloroform-glacial acetic acid(6:5:1)with the detection wave length of 365nm and the control wave length of 322nm.RESULTS The linear range of ferulic acid was 0.4～2.0μg.The mean recovery was 98.4%(n=5)with RSD of 3.71%.CONCLUSION This menhod is accurate,reliable and can be used for the determination of ferulic acid in Xiao-yao-wan.
OBJECTIVE To establish a method of thin layer chromatographic scanning to determine the content of ferulic acid in Xiao-yao-wan.METHODS Silica G thin layer was used,and the mobile phase consisted of benzene-chloroform-glacial acetic acid(6:5:1)with the detection wave length of 365nm and the control wave length of 322nm.RESULTS The linear range of ferulic acid was 0.4～2.0μg.The mean recovery was 98.4%(n=5)with RSD of 3.71%.CONCLUSION This menhod is accurate,reliable and can be used for the determination of ferulic acid in Xiao-yao-wan.
2001, (3): 168-169.
Abstract:
OBJECTIVE To extract and determine content polysaccharides of Snakegourd root.METHODS Using microwave technique,polysaccharides of Snakegourd root were obtained by hotwater extraction and ethanol precipitation,and content was determinated with phenol-vitriolic colorimetry.RESULTS The polysaccharides content of Snakegourd root was 18.3012%,Recovery rate was 98.86%.CONCLUSION The polysaccharides of Snakegourd root has been obtained for the first time by microwave technique.
OBJECTIVE To extract and determine content polysaccharides of Snakegourd root.METHODS Using microwave technique,polysaccharides of Snakegourd root were obtained by hotwater extraction and ethanol precipitation,and content was determinated with phenol-vitriolic colorimetry.RESULTS The polysaccharides content of Snakegourd root was 18.3012%,Recovery rate was 98.86%.CONCLUSION The polysaccharides of Snakegourd root has been obtained for the first time by microwave technique.
2001, (3): 170-171.
Abstract:
OBJECTIVE To establish a method of determination the content of rivanol by Volume-analysis.METHODSThe method of back-dropping was used as main step.The reliability of the Volume-analysis by regression analysis and the label content of samples were measured.RESULTS The liner-regression was good and t he reg ression equation was V=10.01-1.386×10-2C,r=0.9996 The five batch samples were qualified contents.The average of recovery was 100.13%and RSD=1.78%(n=5).CONCLUSION The method is simple and reliable,which can be used to the quality control of rivanol in preparation.
OBJECTIVE To establish a method of determination the content of rivanol by Volume-analysis.METHODSThe method of back-dropping was used as main step.The reliability of the Volume-analysis by regression analysis and the label content of samples were measured.RESULTS The liner-regression was good and t he reg ression equation was V=10.01-1.386×10-2C,r=0.9996 The five batch samples were qualified contents.The average of recovery was 100.13%and RSD=1.78%(n=5).CONCLUSION The method is simple and reliable,which can be used to the quality control of rivanol in preparation.
2001, (3): 174-175.
Abstract:
OBJECTIVE To Study the chemical constituents in the roots of Codonopsis convolvulacea. METHODS Chromatography and spectral analysis were used to isolation the constituents and elucidate their structures. RESULTS Five compounds were isolated from the alcohol extracts of the roots and identified as taraxerone(1),taraxerol(2),shikimic acid(3),syringaresinol(4),stigmasterol(5).CONCLUSION All the compounds are isolated from the roots of this plant for the first time.
OBJECTIVE To Study the chemical constituents in the roots of Codonopsis convolvulacea. METHODS Chromatography and spectral analysis were used to isolation the constituents and elucidate their structures. RESULTS Five compounds were isolated from the alcohol extracts of the roots and identified as taraxerone(1),taraxerol(2),shikimic acid(3),syringaresinol(4),stigmasterol(5).CONCLUSION All the compounds are isolated from the roots of this plant for the first time.
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