Determination of dexamethasone sodium phosphate and ethylparaben in Zhiyang dishuang cream by HPLC of double wavelength
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摘要: 目的 建立双波长HPLC法同时测定止痒地霜中地塞米松磷酸钠和羟苯乙酯的含量。 方法 采用RP-HPLC法,以Wondasil C18(4.6 mm×250 mm,5 μm)为色谱柱,以三乙胺溶液-甲醇-乙腈(52:43:5)为流动相;检测波长为242 nm,流速为1.0 ml/min。 结果 羟苯乙酯和地塞米松磷酸酯分别在21.42~64.26 μg/ml (r=1.0000)和20.84~62.53 μg/ml (r=0.9999)范围内有良好的线性关系,平均回收率分别为99.1%(RSD=0.41%)和99.0%(RSD=0.72%)。 结论 该法简便、准确、重复性好,可用于止痒地霜的质量控制。Abstract: Objective To establish a double wavelength HPLC method for determination of dexamethasone sodium phosphate and ethylparaben in Zhiyang dishuang cream. Methods The HPLC method was adopted with the Wondasil C18(4.6 mm×250 mm,5 μm) and triethylamine solution-methanol-acetonitrile (52:43:5) as the mobile phase at the rate of 1 ml/min.The detection wavelength was 242 nm. Results The linear ranges of ethylparaben and dexamethasone phosphate were 21.42-64.26 μg/ml(r=1.0000)and 20.84-62.53μg/ml(r=0.9999)respectively.The average recoveries of the three contents were 99.1% and 99.0%,while RSD were 0.41% and 0.72%. Conclusion The method is simple,accurate,reproducible and suitable for quality control of Zhiyang dishuang cream.
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Key words:
- Zhiyang dishuang cream /
- dexamethasone sodium phosphate /
- ethylparaben
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