留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

应中央军委要求,2022年9月起,《药学实践杂志》将更名为《药学实践与服务》,双月刊,正文96页;2023年1月起,拟出版月刊,正文64页,数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿!

海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究

唐杰 林厚文 孙凡

唐杰, 林厚文, 孙凡. 海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究[J]. 药学实践与服务, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
引用本文: 唐杰, 林厚文, 孙凡. 海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究[J]. 药学实践与服务, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
TANG Jie, LIN Houwen, SUN Fan. Effect of marine sponge-derived smenospongine on apoptosis in breast cancer MCF7 cells[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
Citation: TANG Jie, LIN Houwen, SUN Fan. Effect of marine sponge-derived smenospongine on apoptosis in breast cancer MCF7 cells[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004

海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究

doi: 10.3969/j.issn.1006-0111.2018.05.004
基金项目: 国家自然科学青年基金项目(81502936)

Effect of marine sponge-derived smenospongine on apoptosis in breast cancer MCF7 cells

  • 摘要: 目的 研究海绵Spongia pertusa Esper来源的smenospongine(Sme)诱导乳腺癌细胞MCF7凋亡的作用机制。 方法 使用CCK-8法检测Sme对MCF7细胞活力的影响;DAPI染色检测凋亡细胞的细胞核形态;使用流式细胞术检测细胞凋亡率和线粒体膜电位;Western blotting检测Sme对Bax、Bcl2、细胞色素C(cytochorome C)、p-p38和p38蛋白水平表达的影响。 结果 CCK-8法检测结果表明,Sme抑制MCF7增殖,IC50值为(16.46 ±0.88)μmol/L;DAPI染色结果和Annexin V-FITC/PI染色结果显示Sme诱导细胞凋亡。随着加药浓度的增加,细胞凋亡率从4.18%逐渐升至21.49%;流式细胞仪检测结果表明Sme引发MCF7细胞内线粒体膜电位下降;Western blotting结果显示Sme激活了内源性凋亡途径和p38丝裂源活化蛋白激酶(MAPK)信号通路。 结论 Sme可能通过激活p38 MAPK通路,诱导细胞内源性凋亡发挥抗乳腺癌作用。
  • [1] KOBAYASHI J. Search for new bioactive marine natural products and application to drug development[J]. Chem Pharm Bull (Tokyo),2016, 64(8):1079-1083.
    [2] MOLINSKI TF, DALISAY DS, LIEVENS SL, et al. Drug development from marine natural products[J]. Nat Rev Drug Discov,2009, 8(1):69-85.
    [3] LI J, GU BB, SUN F, et al. Sesquiterpene quinones/hydroquinones from the Marine Sponge Spongia pertusa Esper[J],J Nat Prod,2017, 80(5):1436-1445.
    [4] KONG D, AOKI S, SOWA Y, et al. Smenospongine, a sesquiterpene aminoquinone from a marine sponge, induces G1 arrest or apoptosis in different leukemia cells[J]. Mar Drugs, 2008, 6(3):480-488.
    [5] AOKI S, KONG D, MATSUI K, et al. Smenospongine, a spongean sesquiterpene aminoquinone, induces erythroid differentiation in K562 cells[J]. Anticancer Drugs,2004, 15(4):363-369.
    [6] AOKI S, KONG D, MATSUI K, et al. Sesquiterpene aminoquinones, from a marine sponge, induce erythroid differentiation in human chronic myelogenous leukemia, K562 cells[J]. Chem Pharm Bull (Tokyo),2004, 52(8):935-937.
    [7] KONG D, YAMORI T, KOBAYASHI M, et al. Antiproliferative and antiangiogenic activities of smenospongine, a marine sponge sesquiterpene aminoquinone[J]. Mar Drugs,2011, 9(2):154-161.
    [8] SMITH RA, COKKINIDES V, BROOKS D, et al. Cancer screening in the United States, 2010:a review of current American Cancer Society guidelines and issues in cancer screening[J]. CA Cancer J Clin,2010, 60(2):99-119.
    [9] QUEIROZ EA, PUUKILA S, EICHLER R, et al. Metformin induces apoptosis and cell cycle arrest mediated by oxidative stress, AMPK and FOXO3a in MCF-7 breast cancer cells[J]. PLoS ONE,2014, 9(5):e98207.
    [10] KONDRACKI ML, GUYOT M. Smenospongine:a cytotoxic and antimicrobial aminoquinone isolated from ja:math sp[J]. Tetrahedron Letters,1987, 28(47):5815-5818.
    [11] LOPEZ J, TAIT SW. Mitochondrial apoptosis:killing cancer using the enemy within[J]. Br J Cancer,2015, 112(6):957-962.
    [12] HUANG HL, CHAO MW, LI YC, et al. MPT0G066, a novel anti-mitotic drug, induces JNK-independent mitotic arrest, JNK-mediated apoptosis, and potentiates antineoplastic effect of cisplatin in ovarian cancer[J]. Sci Rep,2016, 6:31664.
    [13] ROOVERS K, ASSOIAN RK. Integrating the MAP kinase signal into the G1 phase cell cycle machinery[J]. Bioessays,2000, 22(9):818-826.
    [14] ZHANG X, WANG X, WU T, et al. Isoliensinine induces apoptosis in triple-negative human breast cancer cells through ROS generation and p38 MAPK/JNK activation[J]. Sci Rep,2015, 5:12579.
    [15] JUNTTILA MR, LI SP, WESTERMARCK J. Phosphatase-mediated crosstalk between MAPK signaling pathways in the regulation of cell survival[J]. FASEB J,2008, 22(4):954-965.
    [16] SCHROETER H, BOYD CS, AHMED R, et al. c-Jun N-terminal kinase (JNK)-mediated modulation of brain mitochondria function:new target proteins for JNK signalling in mitochondrion-dependent apoptosis[J]. Biochem J,2003, 372(Pt 2):359-369.
  • [1] 刘丽艳, 余小翠, 孙传铎.  纳武利尤单抗治疗非小细胞肺癌有效性及安全性的Meta分析 . 药学实践与服务, 2024, 42(10): 451-456. doi: 10.12206/j.issn.2097-2024.202310044
    [2] 迟文雅, 袁艳, 李伟林, 吴茼妤, 俞媛.  负载骨髓间充质干细胞/白藜芦醇脂质体的水凝胶支架用于创伤性脑损伤治疗 . 药学实践与服务, 2024, 42(): 1-8. doi: 10.12206/j.issn.2097-2024.202406034
    [3] 宋雨桐, 夏德润, 顾珩, 唐少文, 易洪刚, 沃红梅.  帕博利珠单抗与铂类化疗方案在晚期非小细胞肺癌一线治疗中的药物经济学评价 . 药学实践与服务, 2024, 42(8): 334-340. doi: 10.12206/j.issn.2097-2024.202303023
    [4] 修建平, 杨朝爱, 刘禧澳, 潘乾禹, 韦广旭, 王卫星.  全反式维甲酸对肝星状细胞活化及氧化应激的作用和机制探索 . 药学实践与服务, 2024, 42(7): 291-296. doi: 10.12206/j.issn.2097-2024.202312054
    [5] 姜涛, 徐卫凡, 蒋益萍, 夏天爽, 辛海量.  巴戟天丸组方对Aβ损伤成骨细胞的作用及基于网络药理学的机制研究 . 药学实践与服务, 2024, 42(7): 285-290, 296. doi: 10.12206/j.issn.2097-2024.202305011
    [6] 杨媛媛, 安晓强, 许佳捷, 江键, 梁媛媛.  正极性驻极体联合5-氟尿嘧啶对瘢痕成纤维细胞生长抑制的协同作用 . 药学实践与服务, 2024, 42(6): 244-247. doi: 10.12206/j.issn.2097-2024.202310027
    [7] 冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军.  雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055
  • 加载中
计量
  • 文章访问数:  3379
  • HTML全文浏览量:  312
  • PDF下载量:  473
  • 被引次数: 0
出版历程
  • 收稿日期:  2018-03-28
  • 修回日期:  2018-05-11

海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究

doi: 10.3969/j.issn.1006-0111.2018.05.004
    基金项目:  国家自然科学青年基金项目(81502936)

摘要: 目的 研究海绵Spongia pertusa Esper来源的smenospongine(Sme)诱导乳腺癌细胞MCF7凋亡的作用机制。 方法 使用CCK-8法检测Sme对MCF7细胞活力的影响;DAPI染色检测凋亡细胞的细胞核形态;使用流式细胞术检测细胞凋亡率和线粒体膜电位;Western blotting检测Sme对Bax、Bcl2、细胞色素C(cytochorome C)、p-p38和p38蛋白水平表达的影响。 结果 CCK-8法检测结果表明,Sme抑制MCF7增殖,IC50值为(16.46 ±0.88)μmol/L;DAPI染色结果和Annexin V-FITC/PI染色结果显示Sme诱导细胞凋亡。随着加药浓度的增加,细胞凋亡率从4.18%逐渐升至21.49%;流式细胞仪检测结果表明Sme引发MCF7细胞内线粒体膜电位下降;Western blotting结果显示Sme激活了内源性凋亡途径和p38丝裂源活化蛋白激酶(MAPK)信号通路。 结论 Sme可能通过激活p38 MAPK通路,诱导细胞内源性凋亡发挥抗乳腺癌作用。

English Abstract

唐杰, 林厚文, 孙凡. 海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究[J]. 药学实践与服务, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
引用本文: 唐杰, 林厚文, 孙凡. 海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究[J]. 药学实践与服务, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
TANG Jie, LIN Houwen, SUN Fan. Effect of marine sponge-derived smenospongine on apoptosis in breast cancer MCF7 cells[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
Citation: TANG Jie, LIN Houwen, SUN Fan. Effect of marine sponge-derived smenospongine on apoptosis in breast cancer MCF7 cells[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
参考文献 (16)

目录

    /

    返回文章
    返回