摘要:
目的 研究大孔树脂分离纯化栀子豉汤有效成分的工艺条件及优化。 方法 采用紫外分光光度法测定总环烯醚萜苷和总大豆异黄酮的量,以总环烯醚萜苷和总大豆异黄酮的含量作为指标,采用单因素实验考察上样液浓度、上样量、洗脱剂浓度及用量等对分离纯化工艺的影响。 结果 最佳工艺条件:上样液最佳吸附浓度为0.1 g/ml(该浓度以每1 ml中所含生药材质量计),上样量与树脂体积比是2:1,吸附时间2 h,依次用1 BV(柱体积)水,6 BV 20%乙醇洗脱,6 BV 60%乙醇洗脱,收集洗脱液。 结论 该纯化工艺合理、稳定,可推广于工业生产。
Abstract:
Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina. Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry. Taking the content of total iridoid glycosides and total isoflavones as indexes, the effect of the concentration of sample solution, medicinal herbs on the amount of sample, concentration and volume of eluent was investigated by single factor test. Results The optimum separation and purification technology was as following: the concentration of sample solution was 0.1 g/ml, the volume ratio of resin to material drug was 2:1, the adsorption time was 2 h, and the sample was firstly eluted with 1 BV water, then 6 BV 20% ethanol and 60% ethanol, and the eluent was collected. Conclusion This optimized separation and purification technology was reasonable and stable, and it could be extended to large-scale production applications.
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