Rapid identification of chemical components in Artemisiae argyi folium by RRLC-TOFMS
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摘要: 目的 应用快速液相色谱-高分辨飞行时间质谱(RRLC-TOFMS)技术对中药艾叶中化学成分进行快速鉴别。 方法 色谱分离采用 Agilent Eclipse C18色谱柱(2.1 mmü100 mm,1.8 μm);流动相:乙腈(A)-0.1%甲酸水溶液(B),梯度洗脱,A相随时间的变化:5%~30%(0~12 min),30%~80%(12~25 min),80%(25~30 min),进样量 1 μl,流速0.35 ml/min,柱温 40℃。TOFMS、电喷雾离子源(ESI)、正离子模式、质量数扫描范围m/z 100~1 500。 结果 一次性鉴别出艾叶中31种化学成分。 结论 建立基于RRLC-TOFMS技术对艾叶中化学成分快速鉴别的方法,为中药艾叶的质量控制及体内的深入研究奠定基础。Abstract: Objective To identify the chemical components in Artemisiae argyi folium by rapid resolution liquid chromatography-time of flight mass spectrometry (RRLC-TOFMS). Methods The separation was performed on an Agilent Eclipse C18 column (2.1 mmü100 mm,1.8 μm ). The mobile phase consisted of acetonitrile (A) and 0.1% formic acid (B) were in gradient elution.The flowing rate was 0.35 ml/min, the injection volume was 1 μl and the temperature of column was 40℃. Time of flight mass spectrometer (TOFMS) with electro spray ion source (ESI) was applied to qualitative analysis under the positive ion mode,and mass scan range was m/z 100-1 500. Results 31 chemical compounds in Artemisiae argyi folium were identified unequivocally. Conclusion A rapid and efficient RRLC-TOFMS approach for identifying the chemical constituents of Artemisiae argyi folium had been successfully established,which paved a way for quality control and further in vivo studies of Artemisiae argyi folium.
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Key words:
- RRLC /
- Artemisiae argyi folium /
- chemical components /
- TOFMS
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[1] 国家药典委员会.中华人民共和国药典一部[S].北京:中国医药科技出版社,2010:82. [2] 周 峰,秦路平,连佳芳,等.艾叶的化学成分、生物活性和植物资源[J].天然药物,2002,18(2):96-98. [3] 梅全喜,高玉桥.艾叶化学及药理研究进展[J].中成药,2006,28(7):1030-1032. [4] 兰美兵,余永莉,李啸红.贵州产艾叶挥发油的化学成分分析[J].药物分析杂志,2009,29(8):1305-1308. [5] 钱 伟,韩 乐,刘训红,等.野艾叶与艾叶的挥发性成分HSGC-MS比较分析[J].中华中医药学刊,2010,28(7):766-768. [6] 戴小军,刘延庆,梅全喜. 蒿属药用植物药理活性研究进展[J].中药材,2005,28(3):243-247. [7] 吕 磊,张 海,赵 亮,等.RRLC-TOF/MS快速鉴别中药淫羊藿中43种化学成分[J].第二军医大学学报,2011,32(3):941-946. [8] 赵 亮,田文君,吕 磊.HPLC-TOF/MS对中药扶正平消胶囊化学成分的鉴别[J].第二军医大学学报,2012,33(7):770-779. [9] 王锦军,黄兆文,李瑶瑶.艾叶化学成分的研究[J].药学服务与研究,2008,8(6):465-466. [10] Tian F,Liu L,Dou DQ,et al.Chemical comparison of Artemisia borealis and three Chinese Artemisia plant[J].J Chin Pharm Sci,2007(16):27-32. [11] 杨晓军,涂院海.陕西艾叶化学成分的研究[J].天然产物研究与开发,2007,(19):402-403. [12] Lopes-Lutz D,Alviano DS,Alviano CS,et al.Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils[J].Phytochemistry,2008(69):1732-1738. [13] 吉 双,张予川,亓云鹏.艾叶的化学成分[J].沈阳药科大学学报,2009,26(8):617-619. [14] Miron TL,Plaza M,Bahrim G,et al.Chemical composition of bioactive pressurized extracts of Romanian aromatic plants[J].J Chromatogr A,2011,1218(30):4918-4927. [15] 高淑丽,刘丽华,张 阳,等.LC-MS/MS法同时测定连翘叶中4种成分的含量[J].中国药房,2013,24(11):1026-1028. -
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