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LIANG Rui-shu, ZHANG Chuan, ZOU Hao, JIN Lei. Determinatin of chlorogenic acid in hawthorn by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 457-458,477. doi: 10.3969/j.issn.1006-0111.2012.06.018
Citation: LIANG Rui-shu, ZHANG Chuan, ZOU Hao, JIN Lei. Determinatin of chlorogenic acid in hawthorn by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 457-458,477. doi: 10.3969/j.issn.1006-0111.2012.06.018

Determinatin of chlorogenic acid in hawthorn by HPLC

doi: 10.3969/j.issn.1006-0111.2012.06.018
  • Received Date: 2012-06-01
  • Rev Recd Date: 2012-09-12
  • Objective To establish a HPLC method for determination of chlorogenic acid in the hawthorn. Methods Sunfire C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phase was methanol:sodium dihydrogen phosphate(25:75). The flow rate was 1.0 ml/min, and the temperature was at 28℃. The detection wavelength was 327 nm. Results The calibration curve was at the range of 7.03~70.3 μg/ml (r=0.999 6), RSD was 0. 92% (n=6) and the average recovery was 96.92%. Conclusion The method was simple, reliable, accurate for determination of chlorogenic acid in the hawthorn.
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Determinatin of chlorogenic acid in hawthorn by HPLC

doi: 10.3969/j.issn.1006-0111.2012.06.018

Abstract: Objective To establish a HPLC method for determination of chlorogenic acid in the hawthorn. Methods Sunfire C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phase was methanol:sodium dihydrogen phosphate(25:75). The flow rate was 1.0 ml/min, and the temperature was at 28℃. The detection wavelength was 327 nm. Results The calibration curve was at the range of 7.03~70.3 μg/ml (r=0.999 6), RSD was 0. 92% (n=6) and the average recovery was 96.92%. Conclusion The method was simple, reliable, accurate for determination of chlorogenic acid in the hawthorn.

LIANG Rui-shu, ZHANG Chuan, ZOU Hao, JIN Lei. Determinatin of chlorogenic acid in hawthorn by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 457-458,477. doi: 10.3969/j.issn.1006-0111.2012.06.018
Citation: LIANG Rui-shu, ZHANG Chuan, ZOU Hao, JIN Lei. Determinatin of chlorogenic acid in hawthorn by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 457-458,477. doi: 10.3969/j.issn.1006-0111.2012.06.018
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