Determination of ethanol content in intermediate products during the production of blood products by GC
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摘要: 目的 建立气相色谱法测定血液制品生产过程中中间品乙醇含量的方法。 方法 采用DB-624毛细管色谱柱,FID检测器,气化室温度230℃;检测器温度280℃;柱温:40℃,保持5 min后,以10℃/min升温至80℃,再以50℃/min升温至230℃,保持3 min;以正丙醇为内标。 结果 乙醇在0.5%~5%浓度范围内线性关系良好(r=1.000 0),平均回收率为99.28%,RSD为0.01%(n=9)。 结论 该方法简单灵敏,结果准确可靠,可用于血液制品生产过程中对中间品乙醇含量的测定。Abstract: Objective To establish a method for determination of ethanol content in intermediate products during the production of blood products by GC. Methods DB-624 column was used with FID detection.The temperature of the injector and detector was 230℃,280℃,respectively.The column temperature was kept at 40℃ for 5 minutes,then increased to 80℃ at 10℃ per minute,and finally to 230℃ at 50℃ per minute for 3 minutes.N-propanol was used as the internal standard. Results The standard curve was linear in the range of 0.05%-5%(r=1.000 0) for ethanol.The average recovery was 99.28% with RSD of 0.01%(n=9). Conclusion This method was simple,sensitive,accurate and reliable.It is suitable for the determination of ethanol content in intermediate products during the production of blood products.
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Key words:
- intermediate products /
- ethanol /
- blood products /
- GC /
- determination
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