Determination for total flavonoids in compound Huanglian clysis
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摘要: 目的 建立复方黄连灌肠液中总黄酮含量测定方法。 方法 采用紫外-可见分光光度法测定。比较直接测定、AlCl3-CH3COONa显色体系、NaNO2-Al (NO3)3-NaOH显色体系等3种不同显色方法,分析以槲皮素、芦丁、橙皮苷为对照品的波长扫描图谱,探讨最佳测定条件。 结果 最佳显色方案为采用NaNO2-Al (NO3)3-NaOH显色法,以芦丁为对照品,在371 nm波长下进行测定;芦丁在2.488~12.44 μg/ml范围内线性关系良好(r=0.9995),加样回收率平均值为100.43%(RSD=1.33%)。 结论 NaNO2-Al (NO3)3-NaOH显色法适用于复方黄连灌肠液总黄酮的含量测定,该法简便、快速、稳定可靠。
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关键词:
- 复方黄连灌肠液 /
- 紫外-可见分光光度法 /
- 总黄酮 /
- 含量测定
Abstract: Objective To establish a method for the determination of the content of total flavonoids in compound Huanglian clysis. Methods Three reference substances and the three coloration methods were studied to find the ideal coloration method and determination conditions by spectrophotometry. Results The sample was detected at 371nm wavelength by NaNO2-Al(NO3)3-NaOH reaction with rutin as reference substance.A good linear relationship was observed in the concentration range of 2.488~12.44μg/ml for querceetin (r=0.9993),and the average recovery rate was 100.43% with RSD of 1.33%. Conclusion The NaNO2-Al(NO3)3-NaOH reaction was simple,quick,stable and reliable,which was proved to be applicable for the determination of the content of total flavonoids in compound Huanglian clysis. -
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