Determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing Kanggan Granules by HPLC
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摘要: 目的 建立麻杏抗感颗粒中盐酸麻黄碱和盐酸伪麻黄碱的含量测定方法。 方法 采用高效液相色谱(HPLC)法,色谱柱:Wondasil C18(4.6 mm×250 mm,5 μm);流动相:乙腈-0.1%磷酸溶液(5:95);流速:1 ml/min;检测波长:215 nm;柱温:30℃;进样量:10 μl。 结果 盐酸麻黄碱、盐酸伪麻黄碱的质量浓度分别在4.231~42.31 μg/ml(r=0.999 7)、1.187~11.87 μg/ml(r=0.999 9)范围内与各自峰面积线性关系良好,平均加样回收率为分别为98.9%、98.1%;RSD分别为0.48%、0.64%。 结论 该法准确度高、重复性好, 可用于麻杏抗感颗粒中盐酸麻黄碱和盐酸伪麻黄碱的含量测定。Abstract: Objective To establish a method of HPLC for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing Kanggan Granules. Methods Wondasil C18(4.6 mm×250 mm,5 μm)was used at 30℃ and the acetonitrile-0.1% H3PO4 (5:95 ) was the mobile phase with a flow rate of 1.0 ml/min. The detection wavelength was at 215nm. Results The linear range of ephedrine hydrochloride and pseudoephedrine hydrochloride were 4.231-42.31 μg/ml(r=0.999 7)、1.187-11.87 μg/ml(r=0.999 9), the average recovery were 98.9% (RSD=0.48%,n=6) and 98.1%(RSD=0.48%,n=6). Conclusion This method was accurate and good reproducibility, which could be used for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing Kanggan Granules.
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