复元醒脑颗粒的提取工艺研究

孙莉莉; 陈振翼; 秦春霞; 胡翔; 方邦江; 李铁军

doi:10.3969/j.issn.1006-0111.2018.02.006

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复元醒脑颗粒的提取工艺研究

孙莉莉, 陈振翼, 秦春霞, 胡翔, 方邦江, 李铁军

文章导航 > 药学实践与服务 > 2018 > 36(2): 121-125

孙莉莉, 陈振翼, 秦春霞, 胡翔, 方邦江, 李铁军. 复元醒脑颗粒的提取工艺研究[J]. 药学实践与服务, 2018, 36(2): 121-125. doi: 10.3969/j.issn.1006-0111.2018.02.006

引用本文:

孙莉莉, 陈振翼, 秦春霞, 胡翔, 方邦江, 李铁军. 复元醒脑颗粒的提取工艺研究[J]. 药学实践与服务, 2018, 36(2): 121-125. doi: 10.3969/j.issn.1006-0111.2018.02.006

SUN Lili, CHEN Zhenyi, QIN Chunxia, HU Xiang, FANG Bangjiang, LI Tiejun. Study on extraction technology of Fuyuan Xingnao granule[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(2): 121-125. doi: 10.3969/j.issn.1006-0111.2018.02.006

Citation:

SUN Lili, CHEN Zhenyi, QIN Chunxia, HU Xiang, FANG Bangjiang, LI Tiejun. Study on extraction technology of Fuyuan Xingnao granule[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(2): 121-125. doi: 10.3969/j.issn.1006-0111.2018.02.006

复元醒脑颗粒的提取工艺研究

doi: 10.3969/j.issn.1006-0111.2018.02.006

1.
上海市浦东新区浦南医院药剂科, 上海 200125
2.
上海中医药大学附属龙华医院, 上海 200032
3.
上海众胜医药科技有限公司, 上海 200125

基金项目: 上海市进一步加快中医药事业发展三年行动计划（2014-2016年）（ZY3-JSFC-2-2007）

Study on extraction technology of Fuyuan Xingnao granule

1.
Department of Pharmacy, Punan Hospital of Pudong New Area, Shanghai 200125, China
2.
Longhua Hospital Affliated to Shanghai Traditional Chinese Medicine University, Shanghai 200032, China
3.
Shanghai Zhongsheng Pharmaceutical Technology Co., Ltd., Shanghai 200125, China

摘要: 目的研究复元醒脑颗粒的提取工艺及其工艺优化。方法采用常规提取方法结合药效学实验确定初步提取工艺，使用正交试验法对提取工艺进行优化。结果乙醇回流提取后，水煎煮提取、乙醇沉淀，过滤，浓缩制得浸膏。最佳乙醇回流提取方案为提取2次；乙醇浓度为50%；料液比：第1次8倍，第2次6倍；提取时间：第1次120 min，第2次80 min。最佳水煎煮提取方案为提取2次；提取时间：第1次120 min，第2次80 min；提取温度为100℃；液料比：第1次10倍，第2次8倍。最佳乙醇沉淀工艺为乙醇浓度80%，乙醇用量为2倍，提取时间为16 h。结论采用本优化工艺提取的浸膏制成的复元醒脑颗粒，质量符合《中华人民共和国药典》2015年版（四部）（0104颗粒剂）中的要求。
- 复元醒脑颗粒 /
- 提取 /
- 优化 /
- 正交试验
Abstract: Objective To study the extraction technology and optimize the extraction process of Fuyuan Xingnao granule. Methods The preliminary extraction process was designed by routine extraction method combined with the results of pharmacological activity experiment. The extraction process was optimized with orthogonal test. Results The extract was obtained by ethanol reflux extraction, water decoction extraction or ethanol precipitation followed by filtration and concentration. The optimal process for ethanol reflux extraction was extracting twice with 50% ethanol. The ratio of liquor to dry material was 8:1 for the first time and 6:1 for the second time. The extraction time was 120 minutes for the first time and 80 minutes for the second time. The optimal process for water decoction extraction was extracting twice with 120 minutes and 80 minutes each time. The ratio of liquor to dry material was 10:1 for the first time and 8:1 for the second time. The extraction temperature was100℃. The best ethanol precipitation process was 16 hour extraction with 80% ethanol. The ratio of ethanol to dry material was 2:1. Conclusion The quality of Fuyuan Xingnao granule extract with this optimized extraction process was in line with requirements of Pharmacopoeia of the People's Republic of China 2015, 4 Volumes(0104 granule).
- Fuyuan Xingnao granule /
- extraction /
- optimization /
- orthogonal test

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复元醒脑颗粒的提取工艺研究

doi: 10.3969/j.issn.1006-0111.2018.02.006

1. 上海市浦东新区浦南医院药剂科, 上海 200125

2. 上海中医药大学附属龙华医院, 上海 200032

3. 上海众胜医药科技有限公司, 上海 200125

基金项目: 上海市进一步加快中医药事业发展三年行动计划（2014-2016年）（ZY3-JSFC-2-2007）

收稿日期: 2017-02-19
修回日期: 2017-05-19

关键词:

复元醒脑颗粒 /

提取 /

优化 /

正交试验

摘要: 目的研究复元醒脑颗粒的提取工艺及其工艺优化。方法采用常规提取方法结合药效学实验确定初步提取工艺，使用正交试验法对提取工艺进行优化。结果乙醇回流提取后，水煎煮提取、乙醇沉淀，过滤，浓缩制得浸膏。最佳乙醇回流提取方案为提取2次；乙醇浓度为50%；料液比：第1次8倍，第2次6倍；提取时间：第1次120 min，第2次80 min。最佳水煎煮提取方案为提取2次；提取时间：第1次120 min，第2次80 min；提取温度为100℃；液料比：第1次10倍，第2次8倍。最佳乙醇沉淀工艺为乙醇浓度80%，乙醇用量为2倍，提取时间为16 h。结论采用本优化工艺提取的浸膏制成的复元醒脑颗粒，质量符合《中华人民共和国药典》2015年版（四部）（0104颗粒剂）中的要求。

English Abstract

Study on extraction technology of Fuyuan Xingnao granule

1. Department of Pharmacy, Punan Hospital of Pudong New Area, Shanghai 200125, China

2. Longhua Hospital Affliated to Shanghai Traditional Chinese Medicine University, Shanghai 200032, China

3. Shanghai Zhongsheng Pharmaceutical Technology Co., Ltd., Shanghai 200125, China

Received Date: 2017-02-19
Rev Recd Date: 2017-05-19

Keywords:

Abstract: Objective To study the extraction technology and optimize the extraction process of Fuyuan Xingnao granule. Methods The preliminary extraction process was designed by routine extraction method combined with the results of pharmacological activity experiment. The extraction process was optimized with orthogonal test. Results The extract was obtained by ethanol reflux extraction, water decoction extraction or ethanol precipitation followed by filtration and concentration. The optimal process for ethanol reflux extraction was extracting twice with 50% ethanol. The ratio of liquor to dry material was 8:1 for the first time and 6:1 for the second time. The extraction time was 120 minutes for the first time and 80 minutes for the second time. The optimal process for water decoction extraction was extracting twice with 120 minutes and 80 minutes each time. The ratio of liquor to dry material was 10:1 for the first time and 8:1 for the second time. The extraction temperature was100℃. The best ethanol precipitation process was 16 hour extraction with 80% ethanol. The ratio of ethanol to dry material was 2:1. Conclusion The quality of Fuyuan Xingnao granule extract with this optimized extraction process was in line with requirements of Pharmacopoeia of the People's Republic of China 2015, 4 Volumes(0104 granule).

孙莉莉, 陈振翼, 秦春霞, 胡翔, 方邦江, 李铁军. 复元醒脑颗粒的提取工艺研究[J]. 药学实践与服务, 2018, 36(2): 121-125. doi: 10.3969/j.issn.1006-0111.2018.02.006

引用本文:

孙莉莉, 陈振翼, 秦春霞, 胡翔, 方邦江, 李铁军. 复元醒脑颗粒的提取工艺研究[J]. 药学实践与服务, 2018, 36(2): 121-125. doi: 10.3969/j.issn.1006-0111.2018.02.006

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复元醒脑颗粒的提取工艺研究

doi: 10.3969/j.issn.1006-0111.2018.02.006

Study on extraction technology of Fuyuan Xingnao granule

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出版历程

复元醒脑颗粒的提取工艺研究

doi: 10.3969/j.issn.1006-0111.2018.02.006

1. 上海市浦东新区浦南医院药剂科, 上海 200125 2. 上海中医药大学附属龙华医院, 上海 200032 3. 上海众胜医药科技有限公司, 上海 200125

English Abstract

Study on extraction technology of Fuyuan Xingnao granule

1. Department of Pharmacy, Punan Hospital of Pudong New Area, Shanghai 200125, China 2. Longhua Hospital Affliated to Shanghai Traditional Chinese Medicine University, Shanghai 200032, China 3. Shanghai Zhongsheng Pharmaceutical Technology Co., Ltd., Shanghai 200125, China

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目录

1. 上海市浦东新区浦南医院药剂科, 上海 200125

2. 上海中医药大学附属龙华医院, 上海 200032

3. 上海众胜医药科技有限公司, 上海 200125

1. Department of Pharmacy, Punan Hospital of Pudong New Area, Shanghai 200125, China

2. Longhua Hospital Affliated to Shanghai Traditional Chinese Medicine University, Shanghai 200032, China

3. Shanghai Zhongsheng Pharmaceutical Technology Co., Ltd., Shanghai 200125, China