Quality standards for Kangkeling mixture
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摘要: 目的 建立康咳灵合剂的质量标准。 方法 采用薄层色谱法(TLC)对矮地茶、百部进行定性鉴别;采用高效液相色谱法(HPLC)测定岩白菜素的含量。色谱柱为Lichrospher C18柱(4.6 mm×250 mm,5 μm);流动相:甲醇-水(20:80);检测波长:275 nm;柱温:30℃;流速:1 ml/min。 结果 TLC法能准确鉴别矮地茶和百部,斑点清晰;岩白菜素在0.064 8~0.648 μg(r=0.9998)范围内线性关系良好,平均回收率100.24%(RSD为1.9%,n=6)。 结论 本实验建立的方法简便、专属性高、重复性好,结果准确可靠,可用于康咳灵合剂的质量控制。Abstract: Objective To establish quality standards for Kangkeling mixture. Methods Ardisiae Japonicae Herba, Stemonae Radix were identified by TLC. The content of bergenin was determined by HPLC. Lichrospher C18 column(5 μm,4.6 mm×250 mm)was used with methanol-water (20:80) as mobile phase. The flow rate was 1.0 ml/min. Column temperature was 30℃ and the detection wavelength at 275 nm. Results The linear range of bergenin was in the range of 0.064 8-0.648 μg (r=0.999 8). The average recovery was 100.24% (RSD=1.9%, n=6). Conclusion The method is simple, specific and sensitive. It can be used to control the quality of Kangkeling mixture.
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Key words:
- Kangkeling mixture /
- TLC /
- HPLC /
- quality standard
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