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Volume 29 Issue 6
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2011  >  29(6): 460-461

FEI Yang, WANG Hui, LING Shui-hua, ZHANG Guo-qing, ZHANG Zu-yan. Determination of chlorogenic acid content in wujin capsule by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 460-461.
Citation: FEI Yang, WANG Hui, LING Shui-hua, ZHANG Guo-qing, ZHANG Zu-yan. Determination of chlorogenic acid content in wujin capsule by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 460-461.
shu

Determination of chlorogenic acid content in wujin capsule by RP-HPLC

  • 1.

    Department of Pharmacy, Eastern Hepatobilliary Hospital, Second Military Medical University, Shanghai 200438, China

  • 2.

    Shanghai Hufeng biology technology Co, Ltd, Shanghai 200433, China

  • Received Date: 2011-06-27
  • Rev Recd Date: 2011-10-20
  • Objective To establish a RP-HPLC method for determination of chlorogenic acid in wujing capsule. Methods Agilent extend-C18 (4.6 mm×250 mm,5 μm)column was used. The mobile phase was acetonitrile: 0.1%formic acid (8：92). The flow rate was 1.0 ml/min, and the temperature was at 35 ℃. The detection wavelength was 327 nm. Determination of chlorogenic acid in wujin capsule of 5 batches of preparations. Results The calibration curve was at the range of 2.04~102 μg/ml(r=0.999 9), and the average recovery was 104.68 %,and RSD was 1.43%(n=6).5 batches of chlorogenic acid contents in wujin capsule were between 55.08~83.31 μg/g. Conclusion The method was sensitive and accurate, and could be used for the quality control of wujin capsule.
  • [1] 张家俊,胡德禹,宋宝安,等.乌骨藤化学成分和生物活性研究进展[J].贵州大学学报,2007,3(24): 213.
    [2] 于文静,王焕群.HPLC法测定消癌平口服液中绿原酸的含量[J].中国药品标准,2006,3(7):50.
    [3] 王月敏,夏素霞,于绍军,等.乌骨藤药材中绿原酸的含量测定方法研究[J].中国实验方剂学杂志,2007,7(13):12.
    [4] 赵陆华,相秉仁,谭喜莹.高效毛细管电泳法测定乌骨藤药材及其制剂中绿原酸的含量[J].中国现代应用药学杂志,2008,4(25):152.
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Determination of chlorogenic acid content in wujin capsule by RP-HPLC

  • 1. Department of Pharmacy, Eastern Hepatobilliary Hospital, Second Military Medical University, Shanghai 200438, China
  • 2. Shanghai Hufeng biology technology Co, Ltd, Shanghai 200433, China
  • Received Date: 2011-06-27
  • Rev Recd Date: 2011-10-20

Abstract: Objective To establish a RP-HPLC method for determination of chlorogenic acid in wujing capsule. Methods Agilent extend-C18 (4.6 mm×250 mm,5 μm)column was used. The mobile phase was acetonitrile: 0.1%formic acid (8：92). The flow rate was 1.0 ml/min, and the temperature was at 35 ℃. The detection wavelength was 327 nm. Determination of chlorogenic acid in wujin capsule of 5 batches of preparations. Results The calibration curve was at the range of 2.04~102 μg/ml(r=0.999 9), and the average recovery was 104.68 %,and RSD was 1.43%(n=6).5 batches of chlorogenic acid contents in wujin capsule were between 55.08~83.31 μg/g. Conclusion The method was sensitive and accurate, and could be used for the quality control of wujin capsule.

FEI Yang, WANG Hui, LING Shui-hua, ZHANG Guo-qing, ZHANG Zu-yan. Determination of chlorogenic acid content in wujin capsule by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 460-461.
Citation: FEI Yang, WANG Hui, LING Shui-hua, ZHANG Guo-qing, ZHANG Zu-yan. Determination of chlorogenic acid content in wujin capsule by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 460-461.
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