CHEN Yong, LIN Chen, ZHANG Jing, QIU Bin, ZHOU Xin, SONG Hong-tao. Determination of sirolimus in whole blood of beagle dog by UPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 353-355,375.
| Citation:
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CHEN Yong, LIN Chen, ZHANG Jing, QIU Bin, ZHOU Xin, SONG Hong-tao. Determination of sirolimus in whole blood of beagle dog by UPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 353-355,375.
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Determination of sirolimus in whole blood of beagle dog by UPLC-MS/MS
- 1.
Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China;Fujian Medical College, Fuzhou 350101, China
- 2.
Fujian institute for Drug Control, Fuzhou 350025, China
- 3.
Deptment of Pharmacy, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, China
- 4.
Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China
- Received Date: 2010-10-10
- Rev Recd Date:
2011-03-31
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Abstract
Objective To develop a UPLC-MS/MS method for the determination of sirolimus in whole blood of beagle dog. Methods Sirolimus and internal standard Tacrolimus were extracted from whole blood with liquid-liquid extraction,then separated on a Agilent C18 column (50 mm ×2.1 mm,1.7 μm) at the flow-rate of 0.30 ml/min. The mobile phase was Acetonitrile-water (90∶10) and the column temperature was 50 ℃. Electrospray ionization ( ESI) source was applied and operated in the positive ion mode. Multiple reaction monitoring (MRM) mode with the transitions of m/z 936.6→409.7 and m/z 826.6→415.4 were used to quantify sirolimus and the internal standard,respectively. Results The calibration linea range of blood-Sirolimu concentrations was 0.50~12.00 ng/ml(r=0.999 7).The low limit of quantification was 0.50 ng/ml . The intra-and inter-day precisions (RSDs) were less than 15%. The method recoveries were more than 90% . The extraction recoveries were more than 76%. Conclusions The method was specific,sensitive,rapid and suitable for the pharmacokinetic study of sirolimus in whole blood of beagle dog.
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