Determination of sinomenine in Wuten Zhentong capsules by HPLC

Key words: 
• Wuten Zhentong capsule /
• HPLC /
• sinomenine /
• Assay

Abstract: Objective To establish an HPLC method for determining the content of sinomenine in Wuten Zhentong capsule. Methods The sample was analyzed on a Shim-pack CLC-ODS-C_{18(150 mm×4.6 mm,5 μm) column using methanol-phosphate buffer(5 mmol/L sodium hydrogen phosphate solution,adjust pH to 8.0 using 5 mmol/L sodium hypophosphite solution and then adjust pH to 9.0 using 1% triethylamine solution)(5545) as mobile phase at 25℃.The detection wavelength was 262 nm and flow rate 1.0 ml/min. Results Good linearity was shown within the range of 0.92～4.60 μg,the correlation coefficient(r) was 0.999 6,the average recovery rate was 99.21%with RSD of 0.61%(n=6). Conclusion The Results demonstrated that the analytical Methods used for the content determination were reproducible,feasible,convenient and easily performable,which can be adopted as the standard Methods for the quality control of the Wuten Zhentong capsule.}