Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 27 Issue 2
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2009  >  27(2): 94-98

LIU Hou-jia, LI Tie-jun, QIU Yan, RUI Rao-cheng, WEI Li-xin, WU Meng-chao, ZHANG Li. Inhibitory effects of vascular endothelial growth factor expression on human umbilical vein endothelial cells by imperatorin[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 94-98.
Citation: LIU Hou-jia, LI Tie-jun, QIU Yan, RUI Rao-cheng, WEI Li-xin, WU Meng-chao, ZHANG Li. Inhibitory effects of vascular endothelial growth factor expression on human umbilical vein endothelial cells by imperatorin[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 94-98.
shu

Inhibitory effects of vascular endothelial growth factor expression on human umbilical vein endothelial cells by imperatorin

  • 1.

    Changhai Hospital, Secondary Military Medical University, Shanghai 200433, China

  • 2.

    College of Pharmacy, Secondary Military Medical University, Shanghai 200433, China

  • 3.

    Department of Pharmacy, the 454th Hospital of PLA, Nanjing 210002, China

  • 4.

    Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai 200438, China

  • Received Date: 2008-08-28
  • Objective : To study the protective effects of imperatorin(IMP) against human umbilical vein endothelial cells(HUVEC) damage and expression of vascular endothelial growth factor(VEGF) induced by lysophosphatidvlcholine(LPC). Methods : Cell iniury were determined by MTT assay.Vascular endothelial growth factor protein production from HUVEC was determined by enzyme-linked immunosorbent assay(ELISA).VEGF mRNA expression was examined by RT-PCR and realtime PCR. Results :Imperatorin prevented LPC-induced injury in cultured HUVEC in a concentration-dependent manner.LPC induced expression of VEGF protein and VEGF mRNA on HUVEC.Imperatorin could inhibit the expression of VEGF protein an d VEGF mRNA in co-cultured with LPC. Conclusions :LPC could induce cell damage and a strong expression of VEGF on HUVEC.Imperatorin could protect HUVEC against the LPC-induced damage and downregulate VEGF protein and VEGF mRNA expression in cultured HUVEC.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2565) PDF downloads(442) Cited by()

Related
Proportional views

Inhibitory effects of vascular endothelial growth factor expression on human umbilical vein endothelial cells by imperatorin

  • 1. Changhai Hospital, Secondary Military Medical University, Shanghai 200433, China
  • 2. College of Pharmacy, Secondary Military Medical University, Shanghai 200433, China
  • 3. Department of Pharmacy, the 454th Hospital of PLA, Nanjing 210002, China
  • 4. Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai 200438, China
  • Received Date: 2008-08-28

Abstract: Objective : To study the protective effects of imperatorin(IMP) against human umbilical vein endothelial cells(HUVEC) damage and expression of vascular endothelial growth factor(VEGF) induced by lysophosphatidvlcholine(LPC). Methods : Cell iniury were determined by MTT assay.Vascular endothelial growth factor protein production from HUVEC was determined by enzyme-linked immunosorbent assay(ELISA).VEGF mRNA expression was examined by RT-PCR and realtime PCR. Results :Imperatorin prevented LPC-induced injury in cultured HUVEC in a concentration-dependent manner.LPC induced expression of VEGF protein and VEGF mRNA on HUVEC.Imperatorin could inhibit the expression of VEGF protein an d VEGF mRNA in co-cultured with LPC. Conclusions :LPC could induce cell damage and a strong expression of VEGF on HUVEC.Imperatorin could protect HUVEC against the LPC-induced damage and downregulate VEGF protein and VEGF mRNA expression in cultured HUVEC.

LIU Hou-jia, LI Tie-jun, QIU Yan, RUI Rao-cheng, WEI Li-xin, WU Meng-chao, ZHANG Li. Inhibitory effects of vascular endothelial growth factor expression on human umbilical vein endothelial cells by imperatorin[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 94-98.
Citation: LIU Hou-jia, LI Tie-jun, QIU Yan, RUI Rao-cheng, WEI Li-xin, WU Meng-chao, ZHANG Li. Inhibitory effects of vascular endothelial growth factor expression on human umbilical vein endothelial cells by imperatorin[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 94-98.
shu

    HTML

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return