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Volume 25 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2007  >  (4): 238-240

WANG Hui, ZHANG Guo-qing. Determination of ropivacaine in human plasma by reversed-phase high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2007, (4): 238-240.
Citation: WANG Hui, ZHANG Guo-qing. Determination of ropivacaine in human plasma by reversed-phase high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2007, (4): 238-240.
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Determination of ropivacaine in human plasma by reversed-phase high performance liquid chromatography

  • Eastern Hepatobiliary Surgery Hospital of Shanghai,Shanghai 200438,China

  • Received Date: 2006-03-23
  • Objective: To establish an accurate and rapid method for determination of ropivacaine by RP-HPLC. Methods: The sample was treated by the addition of bupivacaine as an internal standard,extraction with aether and n-hexane,and dryed by N2.The residue dissolved in the mobile phase and injected into chromatographic system.The HPLC system consisted of AgilentC18 column（4.6 mm×150 mm,5μm）and mobile phase of 0.045 mol/L KH2PO4（pH3.0）-methol（55:45）.Velocity of flow was 1.0 mL/min.The detection wavelength was 210 nm.Column temperature was 30℃. Results: The linear range was from 0.2509μg/mL to 5.018μg/mL with r of 0.999 6,the average recovery was 102.0%.RSD of Intra-day and Inter-day<15%.The results were accord with methodology. Conclusion: This method is quick and stable for ropivacaine monitoring in clinical research.
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Determination of ropivacaine in human plasma by reversed-phase high performance liquid chromatography

  • Eastern Hepatobiliary Surgery Hospital of Shanghai,Shanghai 200438,China
  • Received Date: 2006-03-23

Abstract: Objective: To establish an accurate and rapid method for determination of ropivacaine by RP-HPLC. Methods: The sample was treated by the addition of bupivacaine as an internal standard,extraction with aether and n-hexane,and dryed by N2.The residue dissolved in the mobile phase and injected into chromatographic system.The HPLC system consisted of AgilentC18 column（4.6 mm×150 mm,5μm）and mobile phase of 0.045 mol/L KH2PO4（pH3.0）-methol（55:45）.Velocity of flow was 1.0 mL/min.The detection wavelength was 210 nm.Column temperature was 30℃. Results: The linear range was from 0.2509μg/mL to 5.018μg/mL with r of 0.999 6,the average recovery was 102.0%.RSD of Intra-day and Inter-day<15%.The results were accord with methodology. Conclusion: This method is quick and stable for ropivacaine monitoring in clinical research.

WANG Hui, ZHANG Guo-qing. Determination of ropivacaine in human plasma by reversed-phase high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2007, (4): 238-240.
Citation: WANG Hui, ZHANG Guo-qing. Determination of ropivacaine in human plasma by reversed-phase high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2007, (4): 238-240.
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