Determination of resorcinol, salicylic acid, benzoic acid in hueizhilin ointment by high-performance liquid chromatography

Key words: 
• high-performance liquid chromatography /
• Hueizhilin ointment determination of content /

Abstract: Objective To establish a method of determination of contents in Hueizhilin ointment. Methods HPLC was used with C₁₈ column as stationary phase and methanol-sodium dihydrogen phosphate (40:60) as mobile phase.The detection wavelength was 254nm.Results The calibration curve is linear between 40.0～200.0μg/mL of resorcinol salicylic acid and benzoic acid.The average recorery was 98.1%, 97.9%,97.7% with RSD of 1.88%,1.82%,1.46%.Conclusion The method is reliable, accurate and can be used for the determination of the contents of Hueizhilin ointment.