XUAN Weidong, CHENG Xi, YE Shuna, XU Dedong. Determination of three flavonoid glycosides in Thesium chinensis Turcz by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 460-463. doi: 10.3969/j.issn.1006-0111.2019.05.014
| Citation:
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XUAN Weidong, CHENG Xi, YE Shuna, XU Dedong. Determination of three flavonoid glycosides in Thesium chinensis Turcz by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 460-463. doi: 10.3969/j.issn.1006-0111.2019.05.014
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Determination of three flavonoid glycosides in Thesium chinensis Turcz by HPLC method
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Department of Pharmacy, Changhai Hospital Affiliated to Naval Medical University, Shanghai 200081, China
- Received Date: 2019-01-23
- Rev Recd Date:
2019-03-06
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Abstract
Objective To establish an assay method for three flavonoid glycosides:kaempferol-3-O-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside,kaempferol-3-O-glucoside and kaemperol-3-O-α-L-rhamnopyranosyl(1→2)-[6-O-acetyl]-β-D-glucopyranoside in Thesium chinensis Turcz by HPLC. Methods Dikma C18 column (520 mm×4.6 mm,5 μm) was used with the mobile phase of acetonitrile-water (21:79,adjusted pH to 4.61 with acetic acid) at a flow rate of 1.0 ml/min.The detection wavelength was set at 346 nm,and the column temperature was 25℃. Results The linear range of those three flavonoid glycosides was 125-200 μg/ml,2.5-40 μg/ml and 5.0-80 μg/ml (r=0.999 9).The average recoveries (n=6) were 101.63%,99.82% and 102.02%.RSD was 1.72%,2.34% and 1.94%(n=6) respectively. Conclusion This method is simple and accurate.It can be used for the quality control of Thesium chinensis Turcz and related preparations.
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