Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 37 Issue 5
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2019  >  37(5): 433-439

ZHOU Jing, WU Gao, MA Fujia. Study on the mechanism of proliferation of human lung cancer A549 cells regulated by hsa-miRNA-30a-5p[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 433-439. doi: 10.3969/j.issn.1006-0111.2019.05.009
Citation: ZHOU Jing, WU Gao, MA Fujia. Study on the mechanism of proliferation of human lung cancer A549 cells regulated by hsa-miRNA-30a-5p[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 433-439. doi: 10.3969/j.issn.1006-0111.2019.05.009
shu

Study on the mechanism of proliferation of human lung cancer A549 cells regulated by hsa-miRNA-30a-5p

doi: 10.3969/j.issn.1006-0111.2019.05.009
  • 1.

    Department of Pharmacy, Ningbo Women's and Children's Hospital, Ningbo, 315012, China

  • 2.

    Hongkou Branch, Changhai Hospital Affiliated to Naval Medical University, Shanghai, 200081, China

  • 3.

    Department of Pharmacy, Naval Special Medical Center, Shanghai 200052, China

  • Received Date: 2018-12-11
  • Rev Recd Date: 2019-04-03
  • Objective To study the regulation and mechanism of hsa-miRNA-30a-5p on the proliferation of human lung cancer A549 cells. Methods Five pairs of lung cancer and para-carcinoma tissues were harvested in clinical and measured for hsa-miRNA-30a-5p and SCARA5 levels by real-time PCR and western blotting,respectively.The theoretical binding site of hsa-miRNA-30a-5p in SCARA5's 3'UTR was predicted by bioinformatics,and validated by luciferase report assay.A549 cells were transfected with pshRNA-SCARA5 and miRNA-30a-5p inhibitor and 48 h later,the proliferation of A549 cells after genetic intervention was assayed by the MTT method,and the cell growth curve was plotted to observe the effect of hsa-miRNA-30a-5p knockdown on cell proliferation. Results For all five pairs of samples tested,hsa-miRNA-30a-5p was higher in the cancer tissues than in the adjacent tissue(P<0.05),and SCARA5 protein was lower in the cancer tissues than in the adjacent tissue (P<0.05).The bioinformatics analysis showed that there was a theoretical binding site of hsa-miRNA-30a-5p in SCARA5's 3'UTR.The luciferase assay showed that miRNA-30a mimics inhibited the luciferase expressed by the luciferase reporter vector carrying a wild-type SCARA5's 3'UTR(pGL3-WT- SCARA5)(P<0.05,vs pGL3-WT-SCARA5 alone),and miRNA-30a-5p inhibitor enhanced the luciferase activity(P<0.05,vs pGL3-WT- SCARA5 alone).No change was observed when miRNA-30a-5p mimics or miRNA-30a-5p inhibitor was co-transfected with the luciferase reporter vector carrying a mutant SCARA5's 3'UTR(pGL3-MT- SCARA5)(P>0.05,vs pGL3-WT- SCARA5 alone).Hsa-miRNA-30a-5p level in A549 cells transfected with miRNA-30a inhibitor for 48 h was significantly decreased(P<0.05,vs cell control or NC),and there was no difference in hsa-miRNA-30a-5p between the negative control and the transfection control(P>0.05).The proliferation of A549 cells was suppressed by transfection with miRNA-30a-5p inhibitor 24-72 h after transfection(P<0.05,vs control or NC). Conclusion Hsa-miRNA-30a-5p could increase the proliferation in A549 cells via suppressing the expression of SCARA5,and transfection of miRNA-30a-5p inhibitor could inhibit the proliferation of A549 cells via up-regulating SCARA5 expression.
  • [1] 陈万青,张思维,邹小农.中国肺癌发病死亡的估计和流行趋势研究[J].中国肺癌杂志,2010,13(5):488-493.
    [2] 李媛秋,代敏,陈元立,等.中国省区水平肺癌死亡率估计方法研究[J].中国肺癌杂志,2011,14(2):120-126.
    [3] BARTEL D P.MicroRNAs:genomics,biogenesis,mechanism,and function[J].Cell,2004,116(2):281-297.
    [4] ISSAC B,GALOIAN K,GUETTOUCHE T,et al.Genome-wide mRNA and miRNA expression data analysis to screen for markers involved in sarcomagenesis in human chondrosarcoma cell lines[J].Genom Data,2014,2:320-324.
    [5] MISHRA S,YADAV T,RANI V.Exploring miRNA based approaches in cancer diagnostics and therapeutics[J].Crit Rev Oncol Hematol,2016,98:12-23.
    [6] WINTHER M,ALSNER J,TRAMM T,et al.Evaluation of miR-21 and miR-375 as prognostic biomarkers in esophageal cancer[J].Acta Oncol,2015,54(9):1582-1591.
    [7] WANG P,GUO X Y,ZONG W,et al.MicroRNA-128b suppresses tumor growth and promotes apoptosis by targeting A2bR in gastric cancer[J].Biochem Biophys Res Commun,2015,467(4):798-804.
    [8] EGELAND N G,LUNDE S R,JONSDOTTIR K,et al.The role of microRNAs as predictors of response to tamoxifen treatment in breast cancer patients[J].Int J Mol Sci,2015,16(10):24243-24275.
    [9] LI L P,GUO Y,CHEN Y Z,et al.The diagnostic efficacy and biological effects of microRNA-29b for colon cancer[J].Technol Cancer Res Treat,2016,15(6):772-779.
    [10] KNUDSEN K N,NIELSEN B S,LINDEBJERG J,et al.MicroRNA-17 is the most up-regulated member of the mir-17-92 cluster during early colon cancer evolution[J].PLoS One,2015,10(10):e0140503.
    [11] I H,CHO J Y.Lung cancer biomarkers[J].Adv Clin Chem,2015,72:107-170.
    [12] CHEN Z Z,WU Y,MENG Q T,et al.Elevated microRNA-25 inhibits cell apoptosis in lung cancer by targeting RGS3[J].In Vitro Cell Dev Biol Anim,2016,52(1):62-67.
    [13] DONG W,YAO C P,TENG X P,et al.MiR-140-3p suppressed cell growth and invasion by downregulating the expression of ATP8A1 in non-small cell lung cancer[J].Tumour Biol,2016,37(3):2973-2985.
    [14] SONG L,LI D,ZHAO Y K,et al.MiR-218 suppressed the growth of lung carcinoma by reducing MEF2D expression[J].Tumour Biol,2016,37(3):2891-2900.
    [15] LIN L,LIN H B,WANG L,et al.MiR-130a regulates macrophage polarization and is associated with non-small cell lung cancer[J].Oncol Rep,2015,34(6):3088-3096.
    [16] SONG Y F,HONG J F,LIU D L,et al.MiR-630 targets LMO3 to regulate cell growth and metastasis in lung cancer[J].Am J Transl Res,2015,7(7):1271-1279.
    [17] MA R Q,WANG C Y,WANG J J,et al.MiRNA-mRNA interaction network in non-small cell lung cancer[J].Interdiscip Sci,2016,8(3):209-219.
    [18] HUANG J,ZHENG D L,QIN F S,et al.Genetic and epigenetic silencing of SCARA5 may contribute to human hepatocellular carcinoma by activating FAK signaling[J].J Clin Invest,2010,120(1):223-241.
    [19] KHAMAS A,ISHIKAWA T,SHIMOKAWA K,et al.Screening for epigenetically masked genes in colorectal cancer Using 5-Aza-2'-deoxycytidine,microarray and gene expression profile[J].Cancer Genom Proteomics,2012,9(2):67-75.
    [20] YAN N,ZHANG S,YANG Y,et al.Therapeutic upregulation of class A scavenger receptor member 5 inhibits tumor growth and metastasis[J].Cancer Sci,2012,103(9):1631-1639.
    [21] LIU J,HU G,CHEN D,et al.Suppression of SCARA5 by Snail1 is essential for EMT-associated cell migration of A549 cells[J].Oncogenesis,2013,2:e73.
    [22] WEI W,YANG Y,CAI J,et al.MiR-30a-5p suppresses tumor metastasis of human colorectal cancer by targeting ITGB3[J].Cell Physiol Biochem,2016,39(3):1165-1176.
    [23] BARANISKIN A,BIRKENKAMP-DEMTRODER K,MAGHNOUJ A,et al.MiR-30a-5p suppresses tumor growth in colon carcinoma by targeting DTL[J].Carcinogenesis,2012,33(4):732-739.
    [24] FRANZETTI G A,LAUD-DUVAL K,BELLANGER D,et al.MiR-30a-5p connects EWS-FLI1 and CD99,two major therapeutic targets in Ewing tumor[J].Oncogene,2013,32(33):3915-3921.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(3491) PDF downloads(314) Cited by()

Related
Proportional views

Study on the mechanism of proliferation of human lung cancer A549 cells regulated by hsa-miRNA-30a-5p

doi: 10.3969/j.issn.1006-0111.2019.05.009
  • 1. Department of Pharmacy, Ningbo Women's and Children's Hospital, Ningbo, 315012, China
  • 2. Hongkou Branch, Changhai Hospital Affiliated to Naval Medical University, Shanghai, 200081, China
  • 3. Department of Pharmacy, Naval Special Medical Center, Shanghai 200052, China
  • Received Date: 2018-12-11
  • Rev Recd Date: 2019-04-03

Abstract: Objective To study the regulation and mechanism of hsa-miRNA-30a-5p on the proliferation of human lung cancer A549 cells. Methods Five pairs of lung cancer and para-carcinoma tissues were harvested in clinical and measured for hsa-miRNA-30a-5p and SCARA5 levels by real-time PCR and western blotting,respectively.The theoretical binding site of hsa-miRNA-30a-5p in SCARA5's 3'UTR was predicted by bioinformatics,and validated by luciferase report assay.A549 cells were transfected with pshRNA-SCARA5 and miRNA-30a-5p inhibitor and 48 h later,the proliferation of A549 cells after genetic intervention was assayed by the MTT method,and the cell growth curve was plotted to observe the effect of hsa-miRNA-30a-5p knockdown on cell proliferation. Results For all five pairs of samples tested,hsa-miRNA-30a-5p was higher in the cancer tissues than in the adjacent tissue(P<0.05),and SCARA5 protein was lower in the cancer tissues than in the adjacent tissue (P<0.05).The bioinformatics analysis showed that there was a theoretical binding site of hsa-miRNA-30a-5p in SCARA5's 3'UTR.The luciferase assay showed that miRNA-30a mimics inhibited the luciferase expressed by the luciferase reporter vector carrying a wild-type SCARA5's 3'UTR(pGL3-WT- SCARA5)(P<0.05,vs pGL3-WT-SCARA5 alone),and miRNA-30a-5p inhibitor enhanced the luciferase activity(P<0.05,vs pGL3-WT- SCARA5 alone).No change was observed when miRNA-30a-5p mimics or miRNA-30a-5p inhibitor was co-transfected with the luciferase reporter vector carrying a mutant SCARA5's 3'UTR(pGL3-MT- SCARA5)(P>0.05,vs pGL3-WT- SCARA5 alone).Hsa-miRNA-30a-5p level in A549 cells transfected with miRNA-30a inhibitor for 48 h was significantly decreased(P<0.05,vs cell control or NC),and there was no difference in hsa-miRNA-30a-5p between the negative control and the transfection control(P>0.05).The proliferation of A549 cells was suppressed by transfection with miRNA-30a-5p inhibitor 24-72 h after transfection(P<0.05,vs control or NC). Conclusion Hsa-miRNA-30a-5p could increase the proliferation in A549 cells via suppressing the expression of SCARA5,and transfection of miRNA-30a-5p inhibitor could inhibit the proliferation of A549 cells via up-regulating SCARA5 expression.

ZHOU Jing, WU Gao, MA Fujia. Study on the mechanism of proliferation of human lung cancer A549 cells regulated by hsa-miRNA-30a-5p[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 433-439. doi: 10.3969/j.issn.1006-0111.2019.05.009
Citation: ZHOU Jing, WU Gao, MA Fujia. Study on the mechanism of proliferation of human lung cancer A549 cells regulated by hsa-miRNA-30a-5p[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 433-439. doi: 10.3969/j.issn.1006-0111.2019.05.009
shu

    HTML

Reference (24)

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return