Determination for total flavonoids in compound Huanglian clysis

NI Xiaoxia; WANG Qingfen; LIU Xiaoling; YE Caifa

doi:10.3969/j.issn.1006-0111.2019.04.013

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Objective To establish a method for the determination of the content of total flavonoids in compound Huanglian clysis. Methods Three reference substances and the three coloration methods were studied to find the ideal coloration method and determination conditions by spectrophotometry. Results The sample was detected at 371nm wavelength by NaNO₂-Al(NO₃)₃-NaOH reaction with rutin as reference substance.A good linear relationship was observed in the concentration range of 2.488~12.44μg/ml for querceetin (r=0.9993),and the average recovery rate was 100.43% with RSD of 1.33%. Conclusion The NaNO₂-Al(NO₃)₃-NaOH reaction was simple,quick,stable and reliable,which was proved to be applicable for the determination of the content of total flavonoids in compound Huanglian clysis.

Determination for total flavonoids in compound Huanglian clysis

Department of Pharmacy, Hospital 909 of Joint Logistics Support Force of PLA/the Affiliated Southeast Hospital of Xiamen University, Zhangzhou 363000, China

Received Date: 2019-01-03

Rev Recd Date: 2019-05-18

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Abstract: Objective To establish a method for the determination of the content of total flavonoids in compound Huanglian clysis. Methods Three reference substances and the three coloration methods were studied to find the ideal coloration method and determination conditions by spectrophotometry. Results The sample was detected at 371nm wavelength by NaNO₂-Al(NO₃)₃-NaOH reaction with rutin as reference substance.A good linear relationship was observed in the concentration range of 2.488~12.44μg/ml for querceetin (r=0.9993),and the average recovery rate was 100.43% with RSD of 1.33%. Conclusion The NaNO₂-Al(NO₃)₃-NaOH reaction was simple,quick,stable and reliable,which was proved to be applicable for the determination of the content of total flavonoids in compound Huanglian clysis.

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Determination for total flavonoids in compound Huanglian clysis

doi: 10.3969/j.issn.1006-0111.2019.04.013

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