The effect of triamcinolone acetonide delivered by microneedle roller on nude mouse model xenografted with human hypertrophic scar

ZHANG Juanjuan; YU Hai; WU Zhihong; XU Shun; ZHANG Lichao

doi:10.3969/j.issn.1006-0111.2019.04.011

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Objective To investigate the effect of triamcinolone acetonide cream (TAC) delivered by microneedle roller on the treatment for human hypertrophic scar. Methods The model was established by xenografting human hypertrophic scar onto the back of nude mouse.Seventy-two nude mice were randomly divided into TAC itself,TAC with microneedle and intralesional injection group with 24 mice in each group.Six nude mice from each group were euthanized at 15,30,45 and 60 days after treatment.Xenografted scars were harvested for histologic analysis.The expression of CD34 and α-smooth muscle actin(α-SMA) were detected by immunohistochemistry.Cell apoptosis was detected by TUNEL method. Results 60 days after the treatment,there was no significant morphology change in TAC group.The scar was shrunk,soft and flat with lighter color in the microneedle and injection groups.The values of CD34 expression,α-smooth muscle actin and cell apoptosis in TAC group were 15.83±1.84,47.36±1.95,and 21.50±3.62,respectively,which had no significant difference compared with those after 15 days treatment(P>0.05).The decrease of the expression of CD34,α-SMA(P<0.05)and the increase of apoptotic cells(P<0.05)were observed 30 days after the microneedle treatment and 15 days after intralesional injection.60 days after the administration,the value of CD34 expression,α-SMA,and cell apoptosis in those two groups were 7.83±0.75,9.87±1.96,26.30±8.48 and 27.23±4.68,34.00±1.55,38.67±3.98,respectively,which had significant difference compared to TAC group(P<0.05).There was no significant difference (P>0.05) between the microneedle group and the injection group in the expression of CD34,α-smooth muscle actin,and cell apoptosis. Conclusion The anti-scar effect of triamcinolone acetonide delivered by microneedle roller was similar to that by intralesional injection in the nude mouse model.

The effect of triamcinolone acetonide delivered by microneedle roller on nude mouse model xenografted with human hypertrophic scar

1. Department of Pharmacy, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai 200071, China;Department of Pharmacy, Shanghai Pudong New District Xinchang Community Health Service Center, Shanghai 201314, China

2. Department of Burns and Plastic Surgery, Shanghai Seventh People Hospital, Shanghai 200137, China

3. Department of Pharmacy, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai 200071, China

Received Date: 2019-02-24

Rev Recd Date: 2019-06-25

Key words:

Abstract: Objective To investigate the effect of triamcinolone acetonide cream (TAC) delivered by microneedle roller on the treatment for human hypertrophic scar. Methods The model was established by xenografting human hypertrophic scar onto the back of nude mouse.Seventy-two nude mice were randomly divided into TAC itself,TAC with microneedle and intralesional injection group with 24 mice in each group.Six nude mice from each group were euthanized at 15,30,45 and 60 days after treatment.Xenografted scars were harvested for histologic analysis.The expression of CD34 and α-smooth muscle actin(α-SMA) were detected by immunohistochemistry.Cell apoptosis was detected by TUNEL method. Results 60 days after the treatment,there was no significant morphology change in TAC group.The scar was shrunk,soft and flat with lighter color in the microneedle and injection groups.The values of CD34 expression,α-smooth muscle actin and cell apoptosis in TAC group were 15.83±1.84,47.36±1.95,and 21.50±3.62,respectively,which had no significant difference compared with those after 15 days treatment(P>0.05).The decrease of the expression of CD34,α-SMA(P<0.05)and the increase of apoptotic cells(P<0.05)were observed 30 days after the microneedle treatment and 15 days after intralesional injection.60 days after the administration,the value of CD34 expression,α-SMA,and cell apoptosis in those two groups were 7.83±0.75,9.87±1.96,26.30±8.48 and 27.23±4.68,34.00±1.55,38.67±3.98,respectively,which had significant difference compared to TAC group(P<0.05).There was no significant difference (P>0.05) between the microneedle group and the injection group in the expression of CD34,α-smooth muscle actin,and cell apoptosis. Conclusion The anti-scar effect of triamcinolone acetonide delivered by microneedle roller was similar to that by intralesional injection in the nude mouse model.

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Reference (10)

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The effect of triamcinolone acetonide delivered by microneedle roller on nude mouse model xenografted with human hypertrophic scar

doi: 10.3969/j.issn.1006-0111.2019.04.011

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