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ZHOU Jing, YE Zhaohui, WU Yanhua. The assay of three triterpenoid saponin extracted from Bessia calthaefolia simultaneously by HPLC-ELSD and their antioxidant effects in vitro[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 337-341,347. doi: 10.3969/j.issn.1006-0111.2019.04.010
Citation: ZHOU Jing, YE Zhaohui, WU Yanhua. The assay of three triterpenoid saponin extracted from Bessia calthaefolia simultaneously by HPLC-ELSD and their antioxidant effects in vitro[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 337-341,347. doi: 10.3969/j.issn.1006-0111.2019.04.010

The assay of three triterpenoid saponin extracted from Bessia calthaefolia simultaneously by HPLC-ELSD and their antioxidant effects in vitro

doi: 10.3969/j.issn.1006-0111.2019.04.010
  • Received Date: 2018-12-20
  • Rev Recd Date: 2019-05-31
  • Objective To establish a method for simultaneous determination of three triterpenoid saponins (BeesiosideⅠ、Ⅱ、Ⅲ),and investigate antioxidant effects of triterpenoid saponin extracted from Bessia calthaefolia. Methods The content assay of triterpenoid saponin extracted from Bessia calthaefolia was conducted by high performance liquid chromatography method with evaporative light-scattering detector (HPLC-ELSD).The methodology validation was performed to confirm the content determination.The free radicals scavenging activities and antioxidant effects of the triterpenoid saponin were evaluated in vitro. Results The HPLC-ELSD method can simultaneously determine the content of Beesioside I,Ⅱ and Ⅲ.It has good linearity,precision,repeatability and stability with high recovery.The triterpenoid saponins showed great activity on scavenging ABTS+、DPPH·and OH free radicals and antioxidant effects. Conclusion The HPLC-ELSD method has good specificity,stability,repeatability and accuracy.This method can be used for content determination and quality standard of three triterpenoid saponins (BeesiosideⅠ、Ⅱ、Ⅲ) from Bessia calthaefolia.The triterpenoid saponins exhibited free radicals scavenging effects and antioxidant effects.
  • [1] 鞠建华.中国特有药用植物铁破锣化学成分及生物活性的研究[D].北京:中国协和医科大学,2000.
    [2] SAKURAI N,NAGAI M,GOTO T,et al.Studies on the constituents of Beesia calthaefolia and Souliea vaginata.IV:Beesioside I,a cyclolanostanol xyloside from the rhizome of Beesia calthaefolia[J].Chem Pharm Bull,1993,41(2):272-275.
    [3] SAKURAI N,NAGAI M,NAGASE H,et al.Studies on the constituents of Beesia calthaefolia and Souliea vaginata.Ⅱ:Beesioside Ⅱ,a cyclolanostanol xyloside from rhizome of Beesia calthaefolia[J].Chem Pharm Bull,1986,34(2):582-589.
    [4] INOUE T,SAKURAI T,NAGAI M,et al.Beesioside Ⅲ,a cyclolanostanol xyloside from rhizome of Beesia calthaefolia and Souliea vaginata[J].Chem Pharm Bull,1985,24(6):1329-1331.
    [5] INOUE T,SAKURAI N,GOTO T,et al.Studies on the constituents of Beesia calthaefolia and Souliea vaginata.Ⅱ:Beesioside IV,a cyclolanostanol xyloside from rhizome of Beesia calthaefolia and Souliea vaginata[J].Heterocycles,1990,30(2):897-904.
    [6] JU J H,LIU D,LIN G,et al.Beesiosides A-F,six new cycloartane triterpene glycosides from Beesia calthaefolia[J].J Nat Prod,2002,65(1):42-47.
    [7] JU J H,LIU D,LIN G,et al.Beesiosides G,H and J-N,seven new cycloartane triterpene glycosides from Beesia calthaefolia[J].J Nat Prod,2002,65(2):147-152.
    [8] 鞠建华,林耕,杨峻山,等.铁破锣皂苷O和P的结构及其药理活性[J].药学学报,2002,37(10):788-792.
    [9] 鞠建华,杨峻山,刘铁东.铁破锣化学成分的研究Ⅱ[J].中国药学杂志,2000,35(3):157-160.
    [10] 周静,苗彩云,姚韵茜.HPLC法快速测定早产儿血清中茶碱的含量[J].中国临床药学杂志,2016,25(6):360-362.
    [11] 梁晓东,张茜,边月娟,等.HPLC测定还脑益聪方及配伍前后何首乌肝毒性成分含量变化[J].中华中医药学刊,2018,36(7):1557-1561.
    [12] 张博闻,姚奇志,文晓娟,等.HPLC测定加味升降散中5种大黄蒽醌类成分含量[J].中华中医药学刊,2018,36(6):1399-1402.
    [13] XIA D Z,YU X F,ZHU Z Y,et al.Antioxidant and antibacterial activity of six edible wild plants (Sonchus spp.) in China[J].Nat Prod Res,2011,25(20):1893-1901.
    [14] 田崇梅,夏道宗,邢梦雨.土茯苓总黄酮与亚硒酸钠联用对铅离子螯合能力及体外抗氧化性的研究[J].中华中医药学刊,2018,36(6):1392-1395.
    [15] 郑丹俊.铁破锣化学成分的研究[D],杭州:浙江大学,2015.
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The assay of three triterpenoid saponin extracted from Bessia calthaefolia simultaneously by HPLC-ELSD and their antioxidant effects in vitro

doi: 10.3969/j.issn.1006-0111.2019.04.010

Abstract: Objective To establish a method for simultaneous determination of three triterpenoid saponins (BeesiosideⅠ、Ⅱ、Ⅲ),and investigate antioxidant effects of triterpenoid saponin extracted from Bessia calthaefolia. Methods The content assay of triterpenoid saponin extracted from Bessia calthaefolia was conducted by high performance liquid chromatography method with evaporative light-scattering detector (HPLC-ELSD).The methodology validation was performed to confirm the content determination.The free radicals scavenging activities and antioxidant effects of the triterpenoid saponin were evaluated in vitro. Results The HPLC-ELSD method can simultaneously determine the content of Beesioside I,Ⅱ and Ⅲ.It has good linearity,precision,repeatability and stability with high recovery.The triterpenoid saponins showed great activity on scavenging ABTS+、DPPH·and OH free radicals and antioxidant effects. Conclusion The HPLC-ELSD method has good specificity,stability,repeatability and accuracy.This method can be used for content determination and quality standard of three triterpenoid saponins (BeesiosideⅠ、Ⅱ、Ⅲ) from Bessia calthaefolia.The triterpenoid saponins exhibited free radicals scavenging effects and antioxidant effects.

ZHOU Jing, YE Zhaohui, WU Yanhua. The assay of three triterpenoid saponin extracted from Bessia calthaefolia simultaneously by HPLC-ELSD and their antioxidant effects in vitro[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 337-341,347. doi: 10.3969/j.issn.1006-0111.2019.04.010
Citation: ZHOU Jing, YE Zhaohui, WU Yanhua. The assay of three triterpenoid saponin extracted from Bessia calthaefolia simultaneously by HPLC-ELSD and their antioxidant effects in vitro[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 337-341,347. doi: 10.3969/j.issn.1006-0111.2019.04.010
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