CAO Qingqing, LI Chengjian, LI Yunqing, GE Jiyun, CHEN Jun, ZHOU Jin, QIAN Xian, ZHAO Liang. Chemical constituents and cytotoxic activities in breast cancer of Euphorbia ebracteolata[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 309-313,317. doi: 10.3969/j.issn.1006-0111.2019.04.005
| Citation:
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CAO Qingqing, LI Chengjian, LI Yunqing, GE Jiyun, CHEN Jun, ZHOU Jin, QIAN Xian, ZHAO Liang. Chemical constituents and cytotoxic activities in breast cancer of Euphorbia ebracteolata[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 309-313,317. doi: 10.3969/j.issn.1006-0111.2019.04.005
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Chemical constituents and cytotoxic activities in breast cancer of Euphorbia ebracteolata
- 1.
Department of Pharmacy, People's Hospital of Shanghai Pudong New District, Shanghai 201299, China
- 2.
Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Naval Medical University, Shanghai 200438, China
- 3.
Department of Pharmacy, Shanxi Provincial Children Hospital, Taiyuan 030013, China
- 4.
Department of Pharmacy, Luodian Hospital of Baoshan District, Shanghai 201908, China
- Received Date: 2018-04-25
- Rev Recd Date:
2019-02-25
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Abstract
Objective To investigate the chemical constituents and cytotoxic activities of breast cancer in the roots of Euphorbia ebracteolata. Methods The chemical constituents were isolated and purified by various chromatographic techniques.The in vitro cytotoxic activities of breast cancer of the isolated compounds were studied by MTT method.Their structures were identified on the basis of spectroscopic analyses and physiochemical properties. Results Ten compounds were isolated from the roots of Euphorbia ebracteolata,and elucidated as 2,4-dihydroxy-6-methoxy-3-form yl-1-acetophenon ( 1 ),2,4-dihydroxy-6-methoxy- 3-methyl-1- acetophenon ( 2 ),ebracteolatain A ( 3 ),3,3'-diacetyl-4,4'-dimethoxy-2,2',6,6'-tetrahydroxy diphenylmethan ( 4 ),jolkinolide B ( 5 ),jolkinolide A ( 6 ),linoleic acid ( 7 ),fischeria A ( 8 ),β-amyrin acetate ( 9 ),nonadecanoic acid monoglyceride ( 10 ).Compound 3 showed cytotoxicity against MDA-MB-231 cell、Sum149 cell、MCF7 cell、ZR-75-1 cell、SKBr3 cell、BT474 cell with IC50values of (6.69±0.128),(5.50±0.126),(5.50±0.126),(7.08±0.111),(8.64±0.197),(5.42±0.113)μmol/L,respectively.Compound 4 had no cytotoxicity on the above cancer cells. Conclusion Compounds 6,7,8,10 were firstly isolated from Euphorbia ebracteolata.Compound 3 showed significant cytotoxicity on breast cancer cells.
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