Protective effects of Gallic acid on ionizing irradiated HIEC cells

SUN Huijuan; ZHAO Tao

doi:10.3969/j.issn.1006-0111.2019.04.003

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Objective To investigate the protective effect and primary mechanism of Gallic acid on ⁶⁰Co-γ ray induced Human Intestinal Epithelial cell (HIEC) damage. Methods HIEC in the exponential phase were inoculated in petri dishes and were randomly divided into sham exposure group and exposure group.The exposure group was exposed to 4Gy ⁶⁰Co-γ ray.CCK-8 method was used to detect cell proliferation ability.Clone formation method was used to observe the effects on clone formation ability,ELISA assay was used to evaluate the resistance effects on the lipid oxidation reaction,and Western-Blot test was used to observe the effects of GA on cycle protein Cyclin D1 and CDK4 in irradiated HIEC cells. Results 1.GA can promote the growth of HIEC cells 12h after GA treatment (P<0.05).But GA has a toxic effect on the HIEC growth 24h and 48h after the treatment.2.Compared with 4Gy ⁶⁰Co-γ ray irradiation exposure group,GA(1,10,20 μmol/L) can increase the proliferation rate of HIEC cell significantly (P< 0.05).3.Compared with 4Gy ⁶⁰Co-γ ray irradiation exposure group,pretreatment with GA(1,20 μmol/L) increased the clone formation rates of HIEC cell significantly (P<0.05).4.Compared with 4Gy ⁶⁰Co-γ ray irradiation exposure group,SOD activity of GA(1,10,20μmol/L)treatment group increased significantly (P<0.05).GSH activity of 10 μmol/L GA treatment group increased significantly (P<0.05).5.The expression level of cell cycle protein Cyclin D1 and CDK4 in GA (1 μmol/L) treatment group were significantly increased compare with γ ray irradiation exposure group. Conclusion GA has protective effect on ⁶⁰Co-γ ray irradiated HIEC cells.The mechanism may be related to the GA's capability as antioxidant,scavenging free radicals,regulating cell cycle and inhibiting cell apoptosis.

Protective effects of Gallic acid on ionizing irradiated HIEC cells

1. Department of Nursing, Weinan Vocational & Technical College, Weinan 714000, China;Department of Radiation Protective Medicine, Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Shaanxi Provincial Key Laboratory of Free Radical Biology and Medicine, Faculty of Military Preventive Medicine, Air Force Medical University, Xi'an 710032, China

2. Department of Radiation Protective Medicine, Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Shaanxi Provincial Key Laboratory of Free Radical Biology and Medicine, Faculty of Military Preventive Medicine, Air Force Medical University, Xi'an 710032, China

Received Date: 2018-11-26

Rev Recd Date: 2019-04-23

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Abstract: Objective To investigate the protective effect and primary mechanism of Gallic acid on ⁶⁰Co-γ ray induced Human Intestinal Epithelial cell (HIEC) damage. Methods HIEC in the exponential phase were inoculated in petri dishes and were randomly divided into sham exposure group and exposure group.The exposure group was exposed to 4Gy ⁶⁰Co-γ ray.CCK-8 method was used to detect cell proliferation ability.Clone formation method was used to observe the effects on clone formation ability,ELISA assay was used to evaluate the resistance effects on the lipid oxidation reaction,and Western-Blot test was used to observe the effects of GA on cycle protein Cyclin D1 and CDK4 in irradiated HIEC cells. Results 1.GA can promote the growth of HIEC cells 12h after GA treatment (P<0.05).But GA has a toxic effect on the HIEC growth 24h and 48h after the treatment.2.Compared with 4Gy ⁶⁰Co-γ ray irradiation exposure group,GA(1,10,20 μmol/L) can increase the proliferation rate of HIEC cell significantly (P< 0.05).3.Compared with 4Gy ⁶⁰Co-γ ray irradiation exposure group,pretreatment with GA(1,20 μmol/L) increased the clone formation rates of HIEC cell significantly (P<0.05).4.Compared with 4Gy ⁶⁰Co-γ ray irradiation exposure group,SOD activity of GA(1,10,20μmol/L)treatment group increased significantly (P<0.05).GSH activity of 10 μmol/L GA treatment group increased significantly (P<0.05).5.The expression level of cell cycle protein Cyclin D1 and CDK4 in GA (1 μmol/L) treatment group were significantly increased compare with γ ray irradiation exposure group. Conclusion GA has protective effect on ⁶⁰Co-γ ray irradiated HIEC cells.The mechanism may be related to the GA's capability as antioxidant,scavenging free radicals,regulating cell cycle and inhibiting cell apoptosis.

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Protective effects of Gallic acid on ionizing irradiated HIEC cells

doi: 10.3969/j.issn.1006-0111.2019.04.003

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