Protective effect of areca catechu linn ethanol extract against hypoxia in H9C2 cells

ZHAO Anpeng; JIN Ting; WANG Rong

doi:10.3969/j.issn.1006-0111.2019.04.002

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Objective To study the protective effect and mechanism of anhydrous ethanol extract of areca catechu linn against hypoxia in H9C2 cells. Methods Myocardial cell hypoxic mold was established by H9C2 cell line.The effect of areca catechu linn anhydrous ethanol extract on cells livability was studied by CCK-8.The intracellular content of MDA,activities of SOD and GSH were measured to determine the protective effect of the extract.The mRNA of Nrf-2 and caspase-3 were detected by real-time PCR to explore the protective mechanism. Results Compared to the normoxic control group,the cell survival rate in hypoxic group was 28.46% (P<0.01) after 24 hour hypoxia.The intracellular content of MDA increased 44.33% (P<0.05).The activities of SOD and GSH decreased by 16.18% and 30.64%,respectively(P<0.05 or P<0.01).The result of RT-PCR showed the activation of Nrf2 with a 1.74 times increase of mRNA expression(P<0.05).Compared with hypoxic group,there was an dose-dependent increase of cell survival rate in H9C2 cells(P<0.01) when treated with areca catechu linn anhydrous ethanol extract during hypoxia.The intracellular activities of SOD and GSH also increased by 14.90% and 28.94%(P<0.05).The relative expression of Nrf2 mRNA decreased significantly by 66% (P<0.05). Conclusion The anhydrous alcohol extract of areca catechu linn has a significant protective effect on H9C2 cells against hypoxia.Its protective mechanism may relate to the improvement of intracellular antioxidant enzyme activity,the reduction of oxidative stress damage and the improvement of cell hypoxic tolerance.

Protective effect of areca catechu linn ethanol extract against hypoxia in H9C2 cells

1. PLA Field Key Laboratory for Prevention and Remediation of Plateau Environmental Damage, Hospital 940 of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou 730000, China

2. PLA Field Key Laboratory for Prevention and Remediation of Plateau Environmental Damage, Hospital 940 of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou 730000, China;College of Pharmacy, Lanzhou University, Lanzhou 730000, China

Received Date: 2018-12-27

Rev Recd Date: 2019-06-24

Key words:

Abstract: Objective To study the protective effect and mechanism of anhydrous ethanol extract of areca catechu linn against hypoxia in H9C2 cells. Methods Myocardial cell hypoxic mold was established by H9C2 cell line.The effect of areca catechu linn anhydrous ethanol extract on cells livability was studied by CCK-8.The intracellular content of MDA,activities of SOD and GSH were measured to determine the protective effect of the extract.The mRNA of Nrf-2 and caspase-3 were detected by real-time PCR to explore the protective mechanism. Results Compared to the normoxic control group,the cell survival rate in hypoxic group was 28.46% (P<0.01) after 24 hour hypoxia.The intracellular content of MDA increased 44.33% (P<0.05).The activities of SOD and GSH decreased by 16.18% and 30.64%,respectively(P<0.05 or P<0.01).The result of RT-PCR showed the activation of Nrf2 with a 1.74 times increase of mRNA expression(P<0.05).Compared with hypoxic group,there was an dose-dependent increase of cell survival rate in H9C2 cells(P<0.01) when treated with areca catechu linn anhydrous ethanol extract during hypoxia.The intracellular activities of SOD and GSH also increased by 14.90% and 28.94%(P<0.05).The relative expression of Nrf2 mRNA decreased significantly by 66% (P<0.05). Conclusion The anhydrous alcohol extract of areca catechu linn has a significant protective effect on H9C2 cells against hypoxia.Its protective mechanism may relate to the improvement of intracellular antioxidant enzyme activity,the reduction of oxidative stress damage and the improvement of cell hypoxic tolerance.

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Protective effect of areca catechu linn ethanol extract against hypoxia in H9C2 cells

doi: 10.3969/j.issn.1006-0111.2019.04.002

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