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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2019  >  37(3): 231-236

WANG Yan, SHU Peng, SHEN Li, DING ming, HU Zhenlin, WU Mingjiang. Study on optimized extraction of Schisandra chinensis and cutaneous cell antioxidative effect of its extract[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(3): 231-236. doi: 10.3969/j.issn.1006-0111.2019.03.008
Citation: WANG Yan, SHU Peng, SHEN Li, DING ming, HU Zhenlin, WU Mingjiang. Study on optimized extraction of Schisandra chinensis and cutaneous cell antioxidative effect of its extract[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(3): 231-236. doi: 10.3969/j.issn.1006-0111.2019.03.008
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Study on optimized extraction of Schisandra chinensis and cutaneous cell antioxidative effect of its extract

doi: 10.3969/j.issn.1006-0111.2019.03.008
  • 1.

    College of Life and Enviromental Science, Wenzhou University, Wenzhou 325035, China

  • 2.

    Infinitus(China) Company Ltd., Guangzhou 510663, China

  • 3.

    LB Cosmeceutical Technology Co., Ltd., Shanghai 200233, China

  • 4.

    School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Jinan 250353, China

  • 5.

    Wenzhou Life Sciences Research Institute, Wenzhou 325035, China

  • Received Date: 2018-10-08
  • Rev Recd Date: 2018-11-26
  • Objective To investigate the anti-oxidative effects of the Schisandra chinensis extract in skin cells and develop the application as skin anti-oxidant. Methods The ethanol extraction process of Schisandra chinensis was optimized with hot water pre-treatment and steam distillation to improve the color and odor of the extract.The tert-butyl hydroperoxide (tBHP)-induced cell oxidative injured model in HaCaT keratinocytes was used as a cellular model to study the protective effects of the extract.The cell viability was assayed with CCK-8 kit.Cell apoptosis was detected with flow cytometry by AnnexinV-FITC and PI staining.The intracellular level of reactive oxygen species (ROS) was measured by 2,7-dichlorodi-hydrofluorescein diacetate (DCFH-DA) assay. Results The Schisandra extract prepared by optimized extraction technology exhibited significant improvements in color and odor.The HaCaT cells pretreated with Schisandra extract effectively reduced the cell apoptosis induced by tBHP.The extract increased the survival rate of HaCaT cells and decreased intracellular ROS level. Conclusion The extract with optimized technology improved the color and odor.Its antioxidant activity was still maintained.It could be developed into a novel skin protectant against oxidative damage.
  • [1] 陈玮莹,温博贵,郑瑞明,等.五味子对CCl4中毒大鼠肝细胞核基质蛋白和结构的保护作用[J].免疫学杂志,2006,22(4):455-459.
    [2] 李坦城,安丽萍,滕昊林,等.五味子提取物的研究现状与进展[J].中国医药科学,2014,4(19):80-82.
    [3] 孙建晨,尹水日,崔泰花,等.北五味子提取液清除DPPH自由基的作用[J].食品科技,2012,37(6):247-250.
    [4] 高翔,陈晓宇,田振坤,等.北五味子萃取物清除DPPH自由基作用研究[J].哈尔滨商业大学学报(自然科学版),2016,32(5):546-548.
    [5] 李振,孟宪纬,管延珍,等.南、北五味子提取物的抑菌及抗氧化作用研究[J].中国调味品,2017,42(1):28-30.
    [6] 曹波,路婷婷,魏菲,等.南五味子提取物对UVB辐射的防护作用观察[J].中国皮肤性病学杂志,2016,30(8):771-775+798.
    [7] LAM PY,YAN CW,CHIU PY,et al.Schisandrin B protects against solar irradiation-induced oxidative stress in rat skin tissue[J].Fitoterapia,2011,82(3):393-400.
    [8] 侯微,魏忠宝,高薇,等.五味子甲素·乙素及丙素对HaCat细胞氧化损伤的保护作用研究[J].安徽农业科学,2013,41(3):1047-1049.
    [9] 田双,严铭铭,邵帅,等.五味子醇提物的提取工艺优化及其体外抗氧化作用评价[J].中国药房,2016,27(28):3976-3978.
    [10] 李霞,贾晓斌,陈彦,等.五味子提取工艺的优化研究[J].中国药房,2007,18(6):424-426.
    [11] LEE HJ,KIM CY.Simultaneous determination of nine lignans using pressurized liquid extraction and HPLC-DAD in the fruits of Schisandra chinensis[J].Food Chem,2010,120(4):1224-1228.
    [12] 金银萍,侯微,高薇,等.五味子抗氧化活性组分筛选[J].中国药房,2016,27(19):2622-2625.
    [13] 蒋益萍,张巧艳,张宏,等.正交试验优选五味子木脂素类成分的提取工艺[J].中成药,2013,35(11):2390-2394.
    [14] 高明清.扇贝多肽经由ROS/JNK/caspase-3通路抑制紫外线诱导的HaCaT细胞凋亡[D].青岛:青岛大学,2007.
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Study on optimized extraction of Schisandra chinensis and cutaneous cell antioxidative effect of its extract

doi: 10.3969/j.issn.1006-0111.2019.03.008
  • 1. College of Life and Enviromental Science, Wenzhou University, Wenzhou 325035, China
  • 2. Infinitus(China) Company Ltd., Guangzhou 510663, China
  • 3. LB Cosmeceutical Technology Co., Ltd., Shanghai 200233, China
  • 4. School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Jinan 250353, China
  • 5. Wenzhou Life Sciences Research Institute, Wenzhou 325035, China
  • Received Date: 2018-10-08
  • Rev Recd Date: 2018-11-26

Abstract: Objective To investigate the anti-oxidative effects of the Schisandra chinensis extract in skin cells and develop the application as skin anti-oxidant. Methods The ethanol extraction process of Schisandra chinensis was optimized with hot water pre-treatment and steam distillation to improve the color and odor of the extract.The tert-butyl hydroperoxide (tBHP)-induced cell oxidative injured model in HaCaT keratinocytes was used as a cellular model to study the protective effects of the extract.The cell viability was assayed with CCK-8 kit.Cell apoptosis was detected with flow cytometry by AnnexinV-FITC and PI staining.The intracellular level of reactive oxygen species (ROS) was measured by 2,7-dichlorodi-hydrofluorescein diacetate (DCFH-DA) assay. Results The Schisandra extract prepared by optimized extraction technology exhibited significant improvements in color and odor.The HaCaT cells pretreated with Schisandra extract effectively reduced the cell apoptosis induced by tBHP.The extract increased the survival rate of HaCaT cells and decreased intracellular ROS level. Conclusion The extract with optimized technology improved the color and odor.Its antioxidant activity was still maintained.It could be developed into a novel skin protectant against oxidative damage.

WANG Yan, SHU Peng, SHEN Li, DING ming, HU Zhenlin, WU Mingjiang. Study on optimized extraction of Schisandra chinensis and cutaneous cell antioxidative effect of its extract[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(3): 231-236. doi: 10.3969/j.issn.1006-0111.2019.03.008
Citation: WANG Yan, SHU Peng, SHEN Li, DING ming, HU Zhenlin, WU Mingjiang. Study on optimized extraction of Schisandra chinensis and cutaneous cell antioxidative effect of its extract[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(3): 231-236. doi: 10.3969/j.issn.1006-0111.2019.03.008
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