Simultaneous assay of 10 active components in compound Jinyinhua granules by HPLC

HE Yue; WANG Siwang; XIAO Huimin

doi:10.3969/j.issn.1006-0111.2019.02.016

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Objective To establish an one step assay method for 10 active components (neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,forsythoside B,forsythoside A,baicalin,wogonoside,baicalein,wogonin)in compound Jinyinhua granules. Methods The Kromasil C₁₈ column (250 mm×4.6 mm，5 μm) was used with the column temperature at 35 ℃ and PDA detection wavelength at 320 nm.A gradient elution with acetonitri-0.1% phosphoric acid was adopted at the flow rate of 0.8 ml/min.The injection volume was 10 μl. Results The good resolution was achieved for the 10 active components.The linear ranges of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,forsythoside B,forsythoside A,baicalin,wogonoside,baicalein,wogonin were 10.20-340.06,7.19-239.81,6.12-203.84,15.00-500.00,20.16-671.85,25.19-839.67,14.61-487.14,10.52-350.66,7.32-244.01,7.61-253.82 μg/ml respectively.The average recoveries (n=6) were between 97.67% and 99.00% and RSDs between 0.94% and 1.37%.The average contents of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,forsythoside B,forsythoside A,baicalin,wogonoside,baicalein,wogonin from 10 batches of preparations were 0.579 0,1.523 5,1.080 9,0.083 1,0.468 0,0.739 6,1.977 9,0.351 5,0.246 8 and 0.256 6 mg/g. Conclusion This simple and accurate method can be used as one of quality control methods for the compound Jinyinhua granules.

Simultaneous assay of 10 active components in compound Jinyinhua granules by HPLC

1. Shaanxi Kang Cheng Pharmaceutical Limited by Share Ltd. Shangluo 726000, China;Department of Chinese Materia Medica and Natural Medicines, Air Force Medical University, Xi'an 710032, China

2. Department of Chinese Materia Medica and Natural Medicines, Air Force Medical University, Xi'an 710032, China

3. Department of Chinese Materia Medica and Natural Medicines, Air Force Medical University, Xi'an 710032, China;Shaanxi Light Biological Technology Co., Ltd. Xi'an 710032, China

Received Date: 2018-07-25

Rev Recd Date: 2018-11-22

Key words:

Abstract: Objective To establish an one step assay method for 10 active components (neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,forsythoside B,forsythoside A,baicalin,wogonoside,baicalein,wogonin)in compound Jinyinhua granules. Methods The Kromasil C₁₈ column (250 mm×4.6 mm，5 μm) was used with the column temperature at 35 ℃ and PDA detection wavelength at 320 nm.A gradient elution with acetonitri-0.1% phosphoric acid was adopted at the flow rate of 0.8 ml/min.The injection volume was 10 μl. Results The good resolution was achieved for the 10 active components.The linear ranges of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,forsythoside B,forsythoside A,baicalin,wogonoside,baicalein,wogonin were 10.20-340.06,7.19-239.81,6.12-203.84,15.00-500.00,20.16-671.85,25.19-839.67,14.61-487.14,10.52-350.66,7.32-244.01,7.61-253.82 μg/ml respectively.The average recoveries (n=6) were between 97.67% and 99.00% and RSDs between 0.94% and 1.37%.The average contents of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,forsythoside B,forsythoside A,baicalin,wogonoside,baicalein,wogonin from 10 batches of preparations were 0.579 0,1.523 5,1.080 9,0.083 1,0.468 0,0.739 6,1.977 9,0.351 5,0.246 8 and 0.256 6 mg/g. Conclusion This simple and accurate method can be used as one of quality control methods for the compound Jinyinhua granules.

HTML

Reference (22)

[1]	姜南辉.金银花化学成分研究[J].中药材,2015,38(2):315-317.
[2]	于洋,宋卫霞,郭庆兰,等.金银花水提取物的化学成分研究[J].中国中药杂志,2015,40(17):3496-3504.
[3]	王磊,刘金贤,赵金娟,等.我国金银花种质资源概述[J].中国现代中药,2012,14(12):28-30.
[4]	朱姮,崔莉,刘倩,等. HPLC-DAD-ESI-Q-TOF/MS法测定金银忍冬花中的化学成分[J].中草药,2017,48(11):2300-2305.
[5]	崔春雨,刘志平,周敏,等. 金银花化学成分的研究[J].广西大学学报(自然科学版),2012,37(3):530-533.
[6]	胡璇,李卫东,贾翎,等. 四倍体金银花药材体外抗菌抗病毒实验研究[J].中国现代中药,2015,17(11):1160-1163+1170.
[7]	张泽生,乌兰.金银花中绿原酸的体外抑菌和抗氧化性的研究[J].天津科技大学学报,2005,20(2):5-8+34.
[8]	张晨曦,刘素香,赵艳敏,等.基于液质联用技术的连翘化学成分分析[J].中草药,2016,47(12):2053-2060.
[9]	史洋,王小平,白吉庆,等.连翘抗菌、抗病毒的药理作用研究[J].中国现代中药,2013,15(11):950-953.
[10]	袁岸,赵梦洁,李燕,等.连翘的药理作用综述[J].中药与临床,2015,6(5):56-59.
[11]	闫丽丽,姜颖.连翘药材主要化学成分及药理作用研究综述[J].中国新技术新产品,2011(11):11.
[12]	王立娟.黄芩药材的高效液相色谱指纹图谱及黄芩苷、黄芩素、汉黄芩素含量的同时测定[J].中国医院药学杂志,2015,35(18):1665-1668.
[13]	王雅芳,李婷,唐正海,等.中药黄芩的化学成分及药理研究进展[J].中华中医药学刊,2015,33(1):206-211.
[14]	郑勇凤,王佳婧,傅超美,王建新.黄芩的化学成分与药理作用研究进展[J].中成药,2016,38(1):141-147.
[15]	刘晓婷,张沂,顾立刚,等.黄芩苷体外抗流感病毒作用的研究[J].天津中医药,2015,32(4):229-233.
[16]	谢林利,周密,陈勇川,等.黄芩苷、黄芩素抑制铜绿假单胞菌生物膜形成的研究[J].中国药房,2010,21(39):3651-3653.
[17]	高光武,李玲.黄芩提取物的抗炎作用及其作用机制研究[J].中国临床药理学杂志,2014,30(6):550-552.
[18]	施高翔,邵菁,汪天明,等.黄芩及其有效成分抗菌作用新进展[J].中国中药杂志,2014,39(19):3713-3718.
[19]	肖炜明,卜平,龚卫娟.汉黄芩素抗肿瘤和免疫调节作用的研究进展[J].中国中药杂志,2014,39(16):3004-3009.
[20]	郭明,伍周玲,王春歌,等.黄芩苷-金属配合物的合成及其抗肿瘤活性研究[J].药学学报,2014,49(3):337-345.
[21]	王雅芳,李婷,唐正海,等.中药黄芩的化学成分及药理研究进展[J].中华中医药学刊,2015,33(1):206-211.
[22]	唐励静,文翔昊,李冲,等.HPLC同时测定复方金银花颗粒中绿原酸、连翘苷、黄芩苷、汉黄芩素、芹菜素的含量[J].中国实验方剂学杂志,2014,20(21):71-74.

Message Board

Simultaneous assay of 10 active components in compound Jinyinhua granules by HPLC

doi: 10.3969/j.issn.1006-0111.2019.02.016

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Related

Proportional views