Determination of voriconazole and its metabolites in human plasma by HPLC

WANG Zhijun; YANG Yunyun; ZHANG Wenjing; WANG Xuebin; SONG Hongjie; WANG Zhuo

doi:10.3969/j.issn.1006-0111.2019.02.012

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Objective To establish a HPLC method for the determination of the voriconazole concentration in human plasma,which can be used for voriconazole monitoring clinically. Methods High performance liquid chromatography (HPLC-UV) detection method was used with papaverine as internal standard.Acetonitrile protein precipitation method was employed to treat plasma samples.The chromatographic column was ACE5C₁₈-AR150 mm×4.6 mm with the mobile phase 0.025 mol/L sodium dihydrogen phosphate (containing triethylamine 400 μl/L,pH=7.0 adjusted by 0.25 mol/L sodium hydroxide) - acetonitrile (67∶33).The flow rate was l ml/min,the column temperature at 40 ℃,the detection wavelength at 255,276 nm (double wavelength detection) and sampling amount 20 μl. Results The retention time of voriconazole nitrogen oxide,voriconazole and papaverine were 4.5,11.3 and 13.7 min respectively.The linear ranges of voriconazole and voriconazole nitrogen oxides in plasma were 0.5-20.0 μg/ml (r=0.999 5).The quantitative lower limits were 0.5 μg/ml with RSD< 11%.The recovery rates of extraction were between 90.3% and 109.9%. Conclusion This simple and accurate method is suitable for clinical monitoring of voriconazole.

Determination of voriconazole and its metabolites in human plasma by HPLC

Department of Pharmacy, Changhai Hospital Affiliated to Naval Medical University, Shanghai 200433

Received Date: 2018-08-03

Rev Recd Date: 2018-11-30

Key words:

Abstract: Objective To establish a HPLC method for the determination of the voriconazole concentration in human plasma,which can be used for voriconazole monitoring clinically. Methods High performance liquid chromatography (HPLC-UV) detection method was used with papaverine as internal standard.Acetonitrile protein precipitation method was employed to treat plasma samples.The chromatographic column was ACE5C₁₈-AR150 mm×4.6 mm with the mobile phase 0.025 mol/L sodium dihydrogen phosphate (containing triethylamine 400 μl/L,pH=7.0 adjusted by 0.25 mol/L sodium hydroxide) - acetonitrile (67∶33).The flow rate was l ml/min,the column temperature at 40 ℃,the detection wavelength at 255,276 nm (double wavelength detection) and sampling amount 20 μl. Results The retention time of voriconazole nitrogen oxide,voriconazole and papaverine were 4.5,11.3 and 13.7 min respectively.The linear ranges of voriconazole and voriconazole nitrogen oxides in plasma were 0.5-20.0 μg/ml (r=0.999 5).The quantitative lower limits were 0.5 μg/ml with RSD< 11%.The recovery rates of extraction were between 90.3% and 109.9%. Conclusion This simple and accurate method is suitable for clinical monitoring of voriconazole.

HTML

Reference (12)

[1]	郭天阳,杜安通,杨雅骊,等.第二代三唑类抗真菌药物的研究进展[J].世界临床药物,2014,35(12):715-718.
[2]	CHUWONGWATTANA S,JANTARAROUNGTONG T,CHITASOMBAT M N,et al.A prospective observational study of CYP2C19 polymorphisms and voriconazole plasma level in adult Thai patients with invasive aspergillosis[J].Drug Metab Pharmacokinet,2016,31(2):117-122.
[3]	XU G Q,ZHU L Q,GE T Y,et al.Pharmacokinetic/pharmacodynamic analysis of voriconazole against Candida spp.and Aspergillus spp.in children,adolescents and adults by Monte Carlo simulation[J].Int J Antimicrob Agents,2016,47(6):439-445.
[4]	CABRAL-GALEANO E,RUIZ-CAMPS I,LEN-ABAD O,et al.Clinical usefulness of therapeutic drug monitoring of voriconazole in a university hospital[J].Enferm Infecc Microbiol Clin,2015,33(5):298-302.
[5]	LI Z W,PENG F H, YAN M,et al.Impact of CYP2C19 genotype and liver function on voriconazole pharmacokinetics in renal transplant recipients[J].Ther Drug Monit,2017,39(4):422-428.
[6]	QI F,ZHU L,LI N,et al.Influence of different proton pump inhibitors on the pharmacokinetics of voriconazole[J].Int J Antimicrob Agents,2017,49(4):403-409.
[7]	WANG Y,WANG T,XIE J,et al. Risk factors for voriconazole-associated hepatotoxicity in patients in the intensive care unit[J].Pharmacotherapy,2016,36(7):757-765.
[8]	BRESSÁN I G,MENDEZ M L,GIMENEZ M I.Validation of a reversed-phase ultra-high-performance liquid chromatographic method with photodiode array detection for the determination of voriconazole in human serum and its application to therapeutic drug monitoring[J].Ther Drug Monit,2018,40(2):276-283.
[9]	LIN S C,LIN S W,CHEN J M,et al.Using sweeping-micellar electrokinetic chromatography to sweeping-micellar electrokinetic[J].Talanta,2010,82(2):653-659.
[10]	PEREA S,PENNICK G J, MODAK A,et al.Comparison of high-performance liquid chromatographic and microbiological methods for determination of voriconazole levels in plasma[J].Antimicrob Agents Chemother,2000,44(5):1209-1213.
[11]	刘学松,何为群,陈文英,等.肺部真菌感染患者伏立康唑血药浓度监测的临床应用[J].国际呼吸杂志,2016,36(20):1521-1526.
[12]	GROLL A H,TOWNSEND R,DESAI A,et al.Drug-drug interactions between triazole antifungal agents used to treat invasive aspergillosis and immunosuppressants metabolized by cytochrome P4503A4[J].Transpl Infect Dis,2017,19(5):e12751.

Message Board

Determination of voriconazole and its metabolites in human plasma by HPLC

doi: 10.3969/j.issn.1006-0111.2019.02.012

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Related

Proportional views