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Volume 37 Issue 2
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2019  >  37(2): 115-120,145

AN Xiaoqiang, YUAN Wang, LIANG Yuanyuan, GUO Xin, LIANG Hejuan, JIANG Jian, CUI Lili. The effects of positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagens and TGF-β in rabbit ear scar tissue[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 115-120,145. doi: 10.3969/j.issn.1006-0111.2019.02.004
Citation: AN Xiaoqiang, YUAN Wang, LIANG Yuanyuan, GUO Xin, LIANG Hejuan, JIANG Jian, CUI Lili. The effects of positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagens and TGF-β in rabbit ear scar tissue[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 115-120,145. doi: 10.3969/j.issn.1006-0111.2019.02.004
shu

The effects of positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagens and TGF-β in rabbit ear scar tissue

doi: 10.3969/j.issn.1006-0111.2019.02.004
  • 1.

    Department of Inorganic Chemistry, School of Pharmacy, Naval Medical University, Shanghai 200433, China

  • 2.

    Department of Physics and Mathematics, Faculty of Health Service, Naval Medical University, Shanghai 200433, China

  • Received Date: 2018-06-15
  • Rev Recd Date: 2018-11-12
  • Objective To study the effects of positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagens and TGF-β in rabbit ear scar tissue. Methods The positive electret 5-fluorouracil (5-FU) patches with surface potential of+5 kV were made by attaching the positive charged polypropylene (PP) electret on the back layer of the 5-FU patch.The positive electrets,5-FU patch and positive electret 5-FU patches were applied to the wound surface on rabbit ear.The scar proliferation index was calculated to study the status of the scar.the HE staining,Masson staining and immunohistochemical methods were used to study the effects of the positive electrets,5-FU patch and the positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagen and TGF-β in the rabbit ear scar tissue. Results ① The hypertrophic scar (HS) was formed after 4 weeks natural healing.② The positive electret will not lead to excessive wound repair.③ Both the 5-FU patch and the positive electret 5-FU patch reduced the expression of type Ⅰ collagen,type Ⅲ collagen and TGF-β in scar tissue and inhibited the growth of HS.④ The positive electret 5-FU patch had better inhibiting effect in HS growth than 5-FU patch. Conclusion The combination of positive electret and 5-FU can achieve better results in inhibiting the growth of hypertrophic scar.
  • [1] ROGUES C.Scars,physiology,classification and assessment[J].Soins:La Revue De Reference Infirmiere,2013,772:30-33.
    [2] LANDEN N X,LI D,STAHLE M.Transition form inflammation to proliferation:a critical step during wound healing[J],Cell Mol Life Sci,2016,73(20):3861-3885.
    [3] ZHANG K,GARNER W,COHEN L,et al.Increased typesⅠand Ⅲ collagen and transforming growth factor-beta 1 mRNA and protein in hypertrophic burn scar[J],J Invest Dermatol,1995,104(5):750-754.
    [4] 周游,王婷,于尔特.增生性瘢痕形成的病理机制和当代治疗现状[J].解剖科学进展,2016,22(4):432-435.
    [5] CALISKAN E,GAMSIZKAN M,ACIKGOZ G,et al.Intralesional treatments for hypertrophic scars:comparison among corticosteroid,5-fluorouracil and botulinum toxin in rabbit ear hypertrophic scar model[J].Eur Rev Med Pharmacol Sci,2016,20(8):1603-1608.
    [6] HUSSAIN A,HAQUE M W,SINGH S K,et al.Optimized permeation enhancer for topical delivery of 5-fluorouracil-loaded elastic liposome using design expert:Part Ⅱ[J].Drug Deliv,2016,23(4):1242-1253.
    [7] WANG X,CAO P,LIU J,et.al.5-Aminolaevulinic acid-based photodynamic therapy restrains pathological hyperplasia of fibroblasts[J].Med Sci Monit,2017,23:46-56.
    [8] 王春晓,郭鑫,徐立丽,等.驻极体促进5-氟尿嘧啶体外经皮渗透[J],高电压技术,2016,42(5):1463-1469.
    [9] 黄平,石岩,郭鑫,等.正极性驻极体环孢菌素A贴剂的开路热刺激放电[J],高电压技术,2015,41(4):1390-1394.
    [10] CUI L,LIANG Y,MA L,et al.The combinational effect of PP electret and monoolein on transdermal delivery of cyclosporine A in vitro[J].J Electrostat,2013,71(3):224-227.
    [11] CUI L,LIU H,MA L,et al.Enhanced transdermal delivery of cyclosporine A by PP electret and ethyl oleate[J].IEEE Trans Dielectr Electr Insul,2012,19(4):1191-1194.
    [12] 徐立丽.含化学促渗剂驻极体5-FU贴剂体外经皮转运和抑制瘢痕生长的动物实验研究[D].上海:第二军医大学,2017:14-49.
    [13] 李荟元.瘢痕的药物治疗进展[J],中国临床康复,2002,6(8):1090-1092.
    [14] 郝冬月,李小静.5-氟尿嘧啶在病理性瘢痕治疗中的应用进展[J],中华医学美学美容杂志,2010,16(4):286-288.
    [15] 孔德平,陈凯.5-氟尿嘧啶对增生性瘢痕的作用研究[J],重庆医学,2015,44(8):1029-1031.
    [16] 杨卫玺,杨敏烈,吕国忠.药物注射法治疗60例增生性瘢痕的疗效观察[J],中国美容医学,2010,l9(3):323-325.
    [17] JIANG J,LIANG Y,DONG F,et al.Study of electret effect of rat skin by thermally stimulated discharge analysis[J].J Electrostat,2012,70(3):258-263.
    [18] 梁媛媛,江键,程亮,等.负极性聚丙烯驻极体美洛昔康贴剂对大鼠皮肤结构的影响[J].解剖学杂志,2009,32(4):502-505.
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The effects of positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagens and TGF-β in rabbit ear scar tissue

doi: 10.3969/j.issn.1006-0111.2019.02.004
  • 1. Department of Inorganic Chemistry, School of Pharmacy, Naval Medical University, Shanghai 200433, China
  • 2. Department of Physics and Mathematics, Faculty of Health Service, Naval Medical University, Shanghai 200433, China
  • Received Date: 2018-06-15
  • Rev Recd Date: 2018-11-12

Abstract: Objective To study the effects of positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagens and TGF-β in rabbit ear scar tissue. Methods The positive electret 5-fluorouracil (5-FU) patches with surface potential of+5 kV were made by attaching the positive charged polypropylene (PP) electret on the back layer of the 5-FU patch.The positive electrets,5-FU patch and positive electret 5-FU patches were applied to the wound surface on rabbit ear.The scar proliferation index was calculated to study the status of the scar.the HE staining,Masson staining and immunohistochemical methods were used to study the effects of the positive electrets,5-FU patch and the positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagen and TGF-β in the rabbit ear scar tissue. Results ① The hypertrophic scar (HS) was formed after 4 weeks natural healing.② The positive electret will not lead to excessive wound repair.③ Both the 5-FU patch and the positive electret 5-FU patch reduced the expression of type Ⅰ collagen,type Ⅲ collagen and TGF-β in scar tissue and inhibited the growth of HS.④ The positive electret 5-FU patch had better inhibiting effect in HS growth than 5-FU patch. Conclusion The combination of positive electret and 5-FU can achieve better results in inhibiting the growth of hypertrophic scar.

AN Xiaoqiang, YUAN Wang, LIANG Yuanyuan, GUO Xin, LIANG Hejuan, JIANG Jian, CUI Lili. The effects of positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagens and TGF-β in rabbit ear scar tissue[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 115-120,145. doi: 10.3969/j.issn.1006-0111.2019.02.004
Citation: AN Xiaoqiang, YUAN Wang, LIANG Yuanyuan, GUO Xin, LIANG Hejuan, JIANG Jian, CUI Lili. The effects of positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagens and TGF-β in rabbit ear scar tissue[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 115-120,145. doi: 10.3969/j.issn.1006-0111.2019.02.004
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