Determination and optimization of extraction process of the content of plantamajoside in plantain

L&#220; Ang; FAN Xin; SU Qian; YU Wenying; YU Chenhuan

doi:10.3969/j.issn.1006-0111.2019.01.018

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Objective To establish a method for determination and optimize the extraction process of the content of plantamajoside in plantain. Methods Plantamajoside content was determined by HPLC.The effects of ethanol concentration, ethanol amount and extraction time on the extraction of plantamajoside from plantain were studied by orthogonal design. Results The calibration curve was linear (r=0.999 6) over the range of 12.52-125.10 μg/ml.The average recovery was 98.57% (RSD=1.45%).The optimum extraction process was as follows:60% ethanol, 10 times volumes, extracted 2 times, 1 h each time. Conclusion The established method was simple, accurate and reproducible for determination of the content of plantamajoside in plantain.The optimal extraction process was stable and feasible.

Determination and optimization of extraction process of the content of plantamajoside in plantain

1. Pharmaceutical Department, Hangzhou First People's Hospital, Hangzhou 310006, China

2. Pharmaceutical Department, Hangzhou Obstetrics and Gynecology Hospital, Hangzhou 310008, China

3. Zhejiang Academy of Medical Sciences, Hangzhou 310013, China

Received Date: 2018-03-13

Rev Recd Date: 2018-10-17

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Abstract: Objective To establish a method for determination and optimize the extraction process of the content of plantamajoside in plantain. Methods Plantamajoside content was determined by HPLC.The effects of ethanol concentration, ethanol amount and extraction time on the extraction of plantamajoside from plantain were studied by orthogonal design. Results The calibration curve was linear (r=0.999 6) over the range of 12.52-125.10 μg/ml.The average recovery was 98.57% (RSD=1.45%).The optimum extraction process was as follows:60% ethanol, 10 times volumes, extracted 2 times, 1 h each time. Conclusion The established method was simple, accurate and reproducible for determination of the content of plantamajoside in plantain.The optimal extraction process was stable and feasible.

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Determination and optimization of extraction process of the content of plantamajoside in plantain

doi: 10.3969/j.issn.1006-0111.2019.01.018

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