Simultaneous determination of 5 B-vitamins in compound trivitamin and calcium pantothenate syrup by UHILIC-MS/MS

REN Qiangqiang; ZHAO Youqin; XU Wen; HUANG Mingqing; YE Shuiguo; SHI Zhenxiang

doi:10.3969/j.issn.1006-0111.2018.06.008

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Objective To establish a ultra hydrophilic interaction chromatography-tandem mass spectrometry (UHILIC-MS/MS) for the simultaneous determination of 5 B-vitamins including thiamine, riboflavin, pyridoxine, nicotinamide and calcium pantothenate in Compound Trivitamin and Calcium Pantothenate Syrup. Methods The chromatographic separation was performed on a Waters ACQUITY UHILIC BEH Amide (2.1 mm×100 mm,1.7 μm) column with the mobile phase consisting of 10 mmol/L ammonium formate water and 90% acetonitrile both containing 0.5% formic acid water in a gradient elution at a flow rate of 0.30 ml/min. An electrospray positive ion multiple reaction monitoring(MRM) was used as a detector. Results Five B-vitamins in the sample achieved excellent linearity calibration curves over the concentration range tested (r>0.998 4) within 5 min. The overall average recovery rates were in the range of 93.27%-100.39% with RSD of 1.41%-4.96%. The contents of thiamine, riboflavin, pyridoxine, nicotinamide and calcium pantothenate in ten samples were 32.40-38.91, 7.002-8.462, 9.677-11.17, 33.64-39.58, 3.276-3.771 mg/250 g, respectively. Conclusion The UHILIC-MS/MS method established in this study could quickly achieve qualitative identification or quantitative detection of five B-vitamins components in Compound Trivitamin and Calcium Pantothenate Syrup, which provided a reliable technical detection method for the development, utilization and quality evaluation of Compound Trivitamin and Calcium Pantothenate Syrup.

Simultaneous determination of 5 B-vitamins in compound trivitamin and calcium pantothenate syrup by UHILIC-MS/MS

1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian Key Laboratory of Chinese Materia Medica, Fuzhou 350122, China

2. Sinopharm Xingsha Pharmaceuticals(Xiamen) Co., Ltd., Xiamen 361028, China

Received Date: 2018-05-11

Rev Recd Date: 2018-10-09

Key words:

Compound Trivitamin and Calcium Pantothenate Syrup /
ultra hydrophilic interaction chromatography-mass spectrometry/mass spectrometry /
thiamine /
riboflavin /
pyridoxine /
nicotinamide /
calcium pantothenate /
content determination

Abstract: Objective To establish a ultra hydrophilic interaction chromatography-tandem mass spectrometry (UHILIC-MS/MS) for the simultaneous determination of 5 B-vitamins including thiamine, riboflavin, pyridoxine, nicotinamide and calcium pantothenate in Compound Trivitamin and Calcium Pantothenate Syrup. Methods The chromatographic separation was performed on a Waters ACQUITY UHILIC BEH Amide (2.1 mm×100 mm,1.7 μm) column with the mobile phase consisting of 10 mmol/L ammonium formate water and 90% acetonitrile both containing 0.5% formic acid water in a gradient elution at a flow rate of 0.30 ml/min. An electrospray positive ion multiple reaction monitoring(MRM) was used as a detector. Results Five B-vitamins in the sample achieved excellent linearity calibration curves over the concentration range tested (r>0.998 4) within 5 min. The overall average recovery rates were in the range of 93.27%-100.39% with RSD of 1.41%-4.96%. The contents of thiamine, riboflavin, pyridoxine, nicotinamide and calcium pantothenate in ten samples were 32.40-38.91, 7.002-8.462, 9.677-11.17, 33.64-39.58, 3.276-3.771 mg/250 g, respectively. Conclusion The UHILIC-MS/MS method established in this study could quickly achieve qualitative identification or quantitative detection of five B-vitamins components in Compound Trivitamin and Calcium Pantothenate Syrup, which provided a reliable technical detection method for the development, utilization and quality evaluation of Compound Trivitamin and Calcium Pantothenate Syrup.

HTML

Reference (15)

[1]	国家药品监督管理局.国家药品标准(试行)[S].WS-10001-(HD-0950)-2002.
[2]	翁水旺,高祖钦. RP-HPLC法测定复方三维右旋泛酸钙糖浆中烟酰胺的含量[J]. 药学进展, 2006, 30(10):457-460.
[3]	翁水旺. RP-HPLC法测定复方三维右旋泛酸钙糖浆中维生素B₁和维生素B₆的含量[J]. 药物分析杂志, 2004, 24(6):645-647.
[4]	陈华龙, 刘旺培. HPLC法测定复合维生素B片维生素B₁、维生素B₂、烟酰胺的含量及含量均匀度[J]. 海峡药学, 2016, 28(2):63-65.
[5]	陈彩云, 魏鲜娥, 蔡伟江,等. 高效液相色谱法同时测定保健食品中泛酸、烟酰胺、维生素B₁、维生素B₂、维生素B₆的含量[J]. 食品安全质量检测学报, 2017, 8(5):1866-1871.
[6]	李彩霞, 苏智阳, 袁文杰. HPLC法测定维生素B族片中3种组分的含量[J]. 海峡药学, 2017, 29(6):54-57.
[7]	解瑞辉. HPLC与UV法测定维生素B₁注射液中维生素B₁含量的比较[J]. 海峡药学, 2017, 29(8):47-49.
[8]	郭强胜, 刘明珂, 禹珊,等. 定量核磁共振法同时测定复合维生素B片中B₁、B₂、烟酰胺和泛酸钙[J]. 波谱学杂志, 2016, 33(3):442-451.
[9]	刘俊杰, 耿春梅, 郭曦,等. 人血清水溶性维生素B₁、B₂、B₆和B₉高效液相色谱串联质谱联用技术测定方法的建立和验证[J]. 中国医院药学杂志, 2017, 37(7):617-620.
[10]	梁瑞强, 王聪, 曹进,等. 高效液相色谱-串联质谱法测定保健食品中10种水溶性维生素[J]. 食品安全质量检测学报, 2016, 7(3):966-974.
[11]	金小青, 姚立新, 薛昆鹏. 亲水作用色谱的发展概述[J]. 化工管理, 2014(27):70-71.
[12]	仇岐枢, 冯建新. 复合维生素B溶液处方的改进及稳定性考察[J]. 中国医院药学杂志, 1997, 17(3):126-127.
[13]	宋永美, 吴玉林. 复合维生素B溶液的化学稳定性分析[J]. 数理医药学杂志, 2005, 18(4):364-366.
[14]	李嘉霖, 高宗华. 维生素B₂片中维生素B₂的测定及稳定性[J]. 光谱实验室, 2012, 29(4):2576-2578.
[15]	孙美玲. 贮存时间、湿度、铜源和添加剂来源对预混料中B族维生素稳定性的影响[D]. 沈阳农业大学, 2016.

Message Board

Simultaneous determination of 5 B-vitamins in compound trivitamin and calcium pantothenate syrup by UHILIC-MS/MS

doi: 10.3969/j.issn.1006-0111.2018.06.008

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Related

Proportional views