QIAN Qingqing, JI Minchun, SUN Guangchun. Study on plasma concentration and pharmacoknetics of lamotrigine in cerebral ischemia model rats by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(5): 443-445,460. doi: 10.3969/j.issn.1006-0111.2018.05.013
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QIAN Qingqing, JI Minchun, SUN Guangchun. Study on plasma concentration and pharmacoknetics of lamotrigine in cerebral ischemia model rats by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(5): 443-445,460. doi: 10.3969/j.issn.1006-0111.2018.05.013
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Study on plasma concentration and pharmacoknetics of lamotrigine in cerebral ischemia model rats by LC-MS/MS
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Department of Pharmacy, Fifth People's Hospital of Shanghai Affiliated to Fudan University, Shanghai 200240, China
- Received Date: 2017-08-30
- Rev Recd Date:
2018-09-03
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Abstract
Objective To establish a sensitive method for determination of concentration of lamotrigine (LTG) in rat plasma and to study the pharmacokinetics by LC-MS/MS. Methods 12 rats were divided evenly into model group and shame-operated group. LTG was given as single dose of 10 mg/kg via intragastrical administration. Blood samples were collected from orbital saphenous venous plexus at 5 min,0.25,0.5,1,2,4,6,8,12,24 and 36 h after dosing. The LTG concentrations in rat plasma were assayed by LC-MS/MS. The pharmacokinetic parameters were calculated by DAS software. Results In shame-operated group,cmax (1 382.87±61.17) μg/L,t1/2 (40.43±6.77) h; AUC0-∞(123.45±70.70) mg·h/L. In model group, cmax(1 713.50±65.11) μg/L, t1/2(73.72±17.46) h, AUC0-∞(188.15±76.37) mg·h/L. Conclusion The method is proved to be suitable for the determination of LTG in rat plasma. LTG concentration reached peak value at 2 h in both groups. However, model group had a longer t1/2 and higher concentration than that in shame-operated group, which is a valuable information for further pharmacodynamics study.
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