Determination of rutaecarpine in Fuzhengpingxiao capsule by HPLC method

LI Shengjian; QIAN Xian; ZHOU Jin; L&#220; Lei; ZHANG Guoqing; ZHAO Liang

doi:10.3969/j.issn.1006-0111.2018.03.018

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Objective To develop a new method to determine the contents of rutaecarpine in Fuzhengpingxiao capsule by HPLC method. Methods Samples were handled by ethanol and extraction with ethyl acetate. The separation was achieved on an Agilent TC-C₁₈column using a mobile phase system of acetonitrile-water (2% Tetrahydrofuran and 0.2% formic acid) at a flow rate of 1.0 ml/min. The temperature of column was 40℃ and the detection wavelength was 240 nm. Results The calibration curves of rutaecarpine showed good linearity in the ranges of 1.18-118 μg/ml, r=0.999 9. The results of intra-day and inter-day precisions were both within 2%, the average additional recovery rate was 94.20%. Conclusion The HPLC method was accurate, specific, sensitive and reproducible, which could be used for quality control of rutaecarpine in the preparation of Fuzhengpingxiao capsule.

Determination of rutaecarpine in Fuzhengpingxiao capsule by HPLC method

Department of Pharmacy, Shanghai Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

Received Date: 2017-05-24

Rev Recd Date: 2018-03-12

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Abstract: Objective To develop a new method to determine the contents of rutaecarpine in Fuzhengpingxiao capsule by HPLC method. Methods Samples were handled by ethanol and extraction with ethyl acetate. The separation was achieved on an Agilent TC-C₁₈column using a mobile phase system of acetonitrile-water (2% Tetrahydrofuran and 0.2% formic acid) at a flow rate of 1.0 ml/min. The temperature of column was 40℃ and the detection wavelength was 240 nm. Results The calibration curves of rutaecarpine showed good linearity in the ranges of 1.18-118 μg/ml, r=0.999 9. The results of intra-day and inter-day precisions were both within 2%, the average additional recovery rate was 94.20%. Conclusion The HPLC method was accurate, specific, sensitive and reproducible, which could be used for quality control of rutaecarpine in the preparation of Fuzhengpingxiao capsule.

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Determination of rutaecarpine in Fuzhengpingxiao capsule by HPLC method

doi: 10.3969/j.issn.1006-0111.2018.03.018

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