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Volume 36 Issue 3
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2018  >  36(3): 215-218,223

LI Ying, ZHU Chunyan. Study on effect mechanism of bioadhesive polymers on panax notoginseng saponins's absorption by microarray technology[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 215-218,223. doi: 10.3969/j.issn.1006-0111.2018.03.005
Citation: LI Ying, ZHU Chunyan. Study on effect mechanism of bioadhesive polymers on panax notoginseng saponins's absorption by microarray technology[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 215-218,223. doi: 10.3969/j.issn.1006-0111.2018.03.005
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Study on effect mechanism of bioadhesive polymers on panax notoginseng saponins's absorption by microarray technology

doi: 10.3969/j.issn.1006-0111.2018.03.005

  • Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China

  • Received Date: 2017-06-26
  • Rev Recd Date: 2018-01-20
  • Objective To study the mechanism of bioadhesive materials (Chitosan, Carbomer and hydroxypropyl methyl cellulose(HPMC)) on promoting panax notoginseng saponins(PNS)'s oral absorption by microarray. Methods The study was divided into four groups.PNS, PNS-chitosan, PNS-carbomer and PNS-HPMC, microarray were used to investigate the change of genes expression level affiliated to the solute carrier transporter(SLC) and the ATP-binding cassette (ABC) after transporting across Caco-2 cell monolayer, to explore the mechanism of bioadhesive materials' effect on PNS's absorption. Results Comparing with PNS group, chitosan and carbomer could significantly increase gene expression level affiliated to SLC transporter, chitosan could decrease multi-resistant genes and P-gp efflux genes expression level affiliated to ABC transporter. HPMC had no obvious effect on SLC and ABC transporter. Conclusion Chitosan and carbomer increase PNS's oral due to genes change affiliated to SLC and ABC transporter that could promote absorption and inhibit efflux. The promotion mechanism of HPMC absorption was that it could prolong retention time on absorption site.
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    [2] Wu JH,Leung GPH,Kwan YW,et al. Suppression of diet-induced hypercholesterolaemia by saponins from Panaxnotoginseng in rats[J].J Funct Foods,2013,5(3):1159-1169.
    [3] Zhou N,Tang Y,Keep RF,et al. Antioxidative effects of Panax notoginseng saponins in brain cells[J]. Phytomedicine,2014,21(10):1189-1195.
    [4] Fan JS,Liu DN,Huang G,et al.Panaxnotoginsengsaponins attenuate atherosclerosis via reciprocal regulation of lipid metabolism and inflammation by inducing liver X receptor alpha expression[J].J Ethnopharmacol,2012,142(3):732-738.
    [5] Xu L,Liu JT,Liu N,et al.Effects of Panaxnotoginsengsaponins on proliferation and apoptosis of vascular smooth muscle cells[J].J Ethnopharmacol,2011,137(1):226-230.
    [6] Li Y,Zhang Y,Zhu CY. Pharmacokinetics and correlation between in vitro release and in vivo absorption of bio-adhesive pellets of panax notoginseng saponins[J]. Chin J Nat Med,2017,15(2):142-151.
    [7] 张运,朱春燕.生物黏附材料对三七总皂苷肠吸收的影响[J].中国药学杂志,2015,50(14):1215-1220.
    [8] Feng H,Chen W,Zhu C. Pharmacokinetics study of bio-adhesive tablet of Panax notoginseng saponins[J]. Int Arch Med,2011,4(1):18.
    [9] 黄聪. 微阵列技术在基因表达分析中的应用[J]. 中国煤炭工业医学杂志,2010,13(3):475-476.
    [10] 廖晓波,周新民,杨进福,等. 应用基因微阵列初步研究风湿性心脏病所致心力衰竭的基因表达谱[J]. 中国循环杂志,2009,24(3):217-220.
    [11] Khan S,Elshaer A,Rahman AS,et al. Genomic evaluation during permeability of indomethacin and its solid dispersion[J]. J Drug Target,2011,19(8):615-623.
    [12] Khan S,Elshaer A,Rahman AS,et al. Systems biology approach to study permeability of paracetamol and its solid dispersion[J]. Int J Pharm,2011,417(1-2):272-279.
    [13] Schlessinger A,Matsson P,Shima JE,et al. Comparison of human solute carriers[J]. Protein Sci,2010,19(3):412-428.
    [14] Yang YH,Dudoit S,Luu P,et al. Normalization for cDNA microarray data:a robust composite method addressing single and multiple slide systematic variation[J]. Nucleic Acids Res,2002,30(4):e15.
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Study on effect mechanism of bioadhesive polymers on panax notoginseng saponins's absorption by microarray technology

doi: 10.3969/j.issn.1006-0111.2018.03.005
  • Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
  • Received Date: 2017-06-26
  • Rev Recd Date: 2018-01-20

Abstract: Objective To study the mechanism of bioadhesive materials (Chitosan, Carbomer and hydroxypropyl methyl cellulose(HPMC)) on promoting panax notoginseng saponins(PNS)'s oral absorption by microarray. Methods The study was divided into four groups.PNS, PNS-chitosan, PNS-carbomer and PNS-HPMC, microarray were used to investigate the change of genes expression level affiliated to the solute carrier transporter(SLC) and the ATP-binding cassette (ABC) after transporting across Caco-2 cell monolayer, to explore the mechanism of bioadhesive materials' effect on PNS's absorption. Results Comparing with PNS group, chitosan and carbomer could significantly increase gene expression level affiliated to SLC transporter, chitosan could decrease multi-resistant genes and P-gp efflux genes expression level affiliated to ABC transporter. HPMC had no obvious effect on SLC and ABC transporter. Conclusion Chitosan and carbomer increase PNS's oral due to genes change affiliated to SLC and ABC transporter that could promote absorption and inhibit efflux. The promotion mechanism of HPMC absorption was that it could prolong retention time on absorption site.

LI Ying, ZHU Chunyan. Study on effect mechanism of bioadhesive polymers on panax notoginseng saponins's absorption by microarray technology[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 215-218,223. doi: 10.3969/j.issn.1006-0111.2018.03.005
Citation: LI Ying, ZHU Chunyan. Study on effect mechanism of bioadhesive polymers on panax notoginseng saponins's absorption by microarray technology[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 215-218,223. doi: 10.3969/j.issn.1006-0111.2018.03.005
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