Research on quality control of Huoguwan

YE Shengying; GUO Qi; LEI Hong; YANG Ying

doi:10.3969/j.issn.1006-0111.2018.02.012

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Objective To establish the quality control method for Huoguwan. Methods TLC was applied to detect the component of Danshen, Dihuang, Danggui, Chuanxiong, Sanqi, Tubiechong, and Zhicaowu in this compound preparation. HPLC was used to measure the concentration of Renshenzaogan Rg₁, Rb₁ and Sanqizaogan R₁. Unitary C₁₈ analytical column(4.6 mm×250 mm,5 μm)was used with acetonitrile-water as the mobile phase (Flow rate:1.0 ml/min, column temperature:30℃, wavelength:203 nm). Results Each component showed distinct spots on TLC without interference. There are good linear relationship for Sanqizhaogan R₁ in the range of 39.92-399.2 μg/ml, Renshenzhaogan Rb₁ in 84.28 -842.8 μg/ml and Renshenzhaogan Rg₁ in 135.86-1 358.6 μg/ml. The recovery was 102.35%, 103.84% and 102.97% respectively. Conclusion The above described procedure was accurate and reproducible, which can be used as a method for the quality control of Huoguwan.

Research on quality control of Huoguwan

1. No. 254 Hospital of PLA, Tianjin 300142, China

2. Institute for Drug and Instrument Control of Beijing Military Area Command, Beijing 100071, China

Received Date: 2017-06-13

Rev Recd Date: 2017-11-14

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Abstract: Objective To establish the quality control method for Huoguwan. Methods TLC was applied to detect the component of Danshen, Dihuang, Danggui, Chuanxiong, Sanqi, Tubiechong, and Zhicaowu in this compound preparation. HPLC was used to measure the concentration of Renshenzaogan Rg₁, Rb₁ and Sanqizaogan R₁. Unitary C₁₈ analytical column(4.6 mm×250 mm,5 μm)was used with acetonitrile-water as the mobile phase (Flow rate:1.0 ml/min, column temperature:30℃, wavelength:203 nm). Results Each component showed distinct spots on TLC without interference. There are good linear relationship for Sanqizhaogan R₁ in the range of 39.92-399.2 μg/ml, Renshenzhaogan Rb₁ in 84.28 -842.8 μg/ml and Renshenzhaogan Rg₁ in 135.86-1 358.6 μg/ml. The recovery was 102.35%, 103.84% and 102.97% respectively. Conclusion The above described procedure was accurate and reproducible, which can be used as a method for the quality control of Huoguwan.

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Reference (8)

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Research on quality control of Huoguwan

doi: 10.3969/j.issn.1006-0111.2018.02.012

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