Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 36 Issue 1
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2018  >  36(1): 64-67

LI Changhui, XU Yueming, ZHANG Qiaoyan, HAN Ting, XIN Hailiang. Analysis of flavonoids contents of hops[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(1): 64-67. doi: 10.3969/j.issn.1006-0111.2018.01.013
Citation: LI Changhui, XU Yueming, ZHANG Qiaoyan, HAN Ting, XIN Hailiang. Analysis of flavonoids contents of hops[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(1): 64-67. doi: 10.3969/j.issn.1006-0111.2018.01.013
shu

Analysis of flavonoids contents of hops

doi: 10.3969/j.issn.1006-0111.2018.01.013
  • 1.

    School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China;School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • 2.

    School of Pharmacy, Jiamusi University, Jiamusi 154007, China;School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • 3.

    School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2017-08-14
  • Rev Recd Date: 2017-10-30
  • Objective To determine the contents of total flavonoids and xanthohumol in hop from 29 different countries and regions. Methods Rutin colorimetric method was used to determine the content of total flavonoids. HPLC-UV method was established for the determination of xanthohumol in hops. HPLC method was performed by Dikma Technologies Diamonsil C18 (250 mm×4.6 mm, 5 μm) column with mobile phase acetonitrile-1% glacial acetic acid solution at the flow rate of 1.0 ml/min. The column temperature was 25℃. The detection wavelength was 370 nm. Results The equation of linear regression of total flavonoids was A=30.345C+0.016 8,r=0.999 9. The equation of linear regression of xanthohumol was A=55 446 C+9040.5,r=0.999 9. Their linear ranges were respectively 20.2-404.0 μg/ml, 2.152-43.040 μg/ml, which indicated a good linear relationship. The RSDs of precision and repeatability were less than 2%. The average recoveries of flavonoids and xanthohumol were respectively 102.71% and 100.21%. Conclusion The contents of total flavonoids and xanthohumol in different hops varieties are significantly different and the import hops was better than the domestic hops in this study.
  • [1] 中国科学院中国植物志编辑委员会.中国植物志第二十三卷第一分册[M]. 北京:科学出版社,1998:221.
    [2] 徐基平,张霞,刘海英,等. 啤酒花的地理分布与中国的野生啤酒花资源[J]. 干旱区研究,2008,22(1):179-183.
    [3] Zanoli P, Zavatti M. Pharmacognostic and pharmacological profile of Humulus lupulus L[J]. J Ethnopharmacol,2008,116(3):383-396.
    [4] 李隽,崔承彬,蔡兵,等. 啤酒花黄酮的研究进展[J]. 中草药, 2008,39(7):1110-1114.
    [5] 朱晓建,段开红,张五九,等. 不同种类啤酒花中黄腐酚含量的研究[J]. 食品与发酵工业,2008,34(10):137-140.
    [6] 冉生斌. 甘肃省啤酒花产业发展现状、存在问题及发展对策[J]. 浙江农业科学,2013,(11):1532-1535.
    [7] 王仿,张霞,刘海英,等. 新疆野生啤酒花总黄酮含量的比较研究[J]. 酿酒科技,2008,(12):27-29.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2596) PDF downloads(680) Cited by()

Related
Proportional views

Analysis of flavonoids contents of hops

doi: 10.3969/j.issn.1006-0111.2018.01.013
  • 1. School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China;School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • 2. School of Pharmacy, Jiamusi University, Jiamusi 154007, China;School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • 3. School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2017-08-14
  • Rev Recd Date: 2017-10-30

Abstract: Objective To determine the contents of total flavonoids and xanthohumol in hop from 29 different countries and regions. Methods Rutin colorimetric method was used to determine the content of total flavonoids. HPLC-UV method was established for the determination of xanthohumol in hops. HPLC method was performed by Dikma Technologies Diamonsil C18 (250 mm×4.6 mm, 5 μm) column with mobile phase acetonitrile-1% glacial acetic acid solution at the flow rate of 1.0 ml/min. The column temperature was 25℃. The detection wavelength was 370 nm. Results The equation of linear regression of total flavonoids was A=30.345C+0.016 8,r=0.999 9. The equation of linear regression of xanthohumol was A=55 446 C+9040.5,r=0.999 9. Their linear ranges were respectively 20.2-404.0 μg/ml, 2.152-43.040 μg/ml, which indicated a good linear relationship. The RSDs of precision and repeatability were less than 2%. The average recoveries of flavonoids and xanthohumol were respectively 102.71% and 100.21%. Conclusion The contents of total flavonoids and xanthohumol in different hops varieties are significantly different and the import hops was better than the domestic hops in this study.

LI Changhui, XU Yueming, ZHANG Qiaoyan, HAN Ting, XIN Hailiang. Analysis of flavonoids contents of hops[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(1): 64-67. doi: 10.3969/j.issn.1006-0111.2018.01.013
Citation: LI Changhui, XU Yueming, ZHANG Qiaoyan, HAN Ting, XIN Hailiang. Analysis of flavonoids contents of hops[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(1): 64-67. doi: 10.3969/j.issn.1006-0111.2018.01.013
shu

    HTML

Reference (7)

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return