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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2017  >  35(6): 526-529

DONG Ruifang, WANG Zheng, CHEN Ling, SHU Zhiheng, HAN Ting, QIN Luping. Content variation of four flavonoid glycosides in Hippophae rhamnoides L. leaves before and after fermentation assayed by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 526-529. doi: 10.3969/j.issn.1006-0111.2017.06.011
Citation: DONG Ruifang, WANG Zheng, CHEN Ling, SHU Zhiheng, HAN Ting, QIN Luping. Content variation of four flavonoid glycosides in Hippophae rhamnoides L. leaves before and after fermentation assayed by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 526-529. doi: 10.3969/j.issn.1006-0111.2017.06.011
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Content variation of four flavonoid glycosides in Hippophae rhamnoides L. leaves before and after fermentation assayed by RP-HPLC

doi: 10.3969/j.issn.1006-0111.2017.06.011
  • 1.

    College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • 2.

    Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • 3.

    Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;Department of Pharmacognosy, School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China

  • Received Date: 2016-05-30
  • Rev Recd Date: 2017-09-11
  • Objective To compare the changes of four flavonoid glycosides in the leaves of Hippophae rhamnoides L. before and after fermentation. Methods The water extract of Hippophae rhamnoides L. leaves and its fermented tea were concentrated and desiccated. The dry extracts were dissolved in 70% ethanol. The chromatographic separation was performed with RP-HPLC method on an Extend-C18 column (4.6 mm×250 mm, 5 μm). Acetonitrile-0.1% formic acid was selected as mobile phase at the flow rate of 1.0 ml/min. The detection wavelength was 356 nm and the column temperature was 30℃. Results The rutin content was high in the leaves of Hippophae rhamnoides L. After fermentation, isoquercitrin content was increased, while the contents of rutin and narcissoside were reduced and isorhamnetin-3-O-glucoside stayed unchanged. There was a good linear relationship between the concentration and peak areas of the four compounds (r>0.9997). The average recoveries were between 96%-103%. Conclusion This established method is rapid and reliable, which can be used for the quality control of Hippophae rhamnoides L. leaves and its fermented tea.
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    [2] 陈雏.青藏高原沙棘属植物资源与品质评价[D].成都:四川大学,2007.
    [3] Wagner H,Ulrich-Merzenich G. Evidence and Rational Based Research on Chinese Drugs[M]. Germany:Springer Vienna, 2013:181-236.
    [4] Ganju L, Padwad Y, Singh R, et al. Anti-inflammatory activity of Seabuckthorn (Hippophae rhamnoides) leaves[J]. Int Immunopharmacol, 2005, 5(12):1675-1684.
    [5] Yogendra Kumar MS, Tirpude RJ, Maheshwari DT, et al. Antioxidant and antimicrobial properties of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves in vitro[J]. Food Chem, 2013, 141(4):3443-3450.
    [6] Ni W, Gao T, Wang H, et al. Anti-fatigue activity of polysaccharides from the fruits of four Tibetan plateau indigenous medicinal plants[J]. J Ethnopharmacol, 2013, 150(2):529-535.
    [7] 国家药典委员会.中华人民共和国药典(2015年版)一部[S].北京:中国医药科技出版社,2015:184-185.
    [8] 李春英,王微,赵春建,等.沙棘不同部位总黄酮含量的测定及比较[J].植物研究,2005, 25(4):453-456.
    [9] 郁长治, 郑永刚, 吴旭东, 等. RP-HPLC法测定沙棘叶中黄酮苷的含量[J].中国当代医药, 2012, 19(11):51-52.
    [10] Zu Y, Li C, Fu Y, et al. Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD[J]. J Pharm Biomed Anal, 2006, 41(3):714-719.
    [11] 李坤, 王明芳, 薛非非, 等. 荷叶炒炭过程中金丝桃苷, 异槲皮苷与槲皮素含量变化规律研究[J]. 世界科学技术-中医药现代化, 2016(1):112-117.
    [12] 马志强,刘彬丽,刘璐,等.不同厂家舒血宁注射液中芦丁和水仙苷的HPLC含量测定[J].北京中医药大学学报,2013,36(11):768-770.
    [13] Barreca D, Laganà G, Leuzzi U, et al. Evaluation of the nutraceutical, antioxidant and cytoprotective properties of ripe pistachio (Pistacia vera L., variety Bronte) hulls[J]. Food Chem, 2016, 196:493-502.
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Content variation of four flavonoid glycosides in Hippophae rhamnoides L. leaves before and after fermentation assayed by RP-HPLC

doi: 10.3969/j.issn.1006-0111.2017.06.011
  • 1. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • 2. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • 3. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;Department of Pharmacognosy, School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China
  • Received Date: 2016-05-30
  • Rev Recd Date: 2017-09-11

Abstract: Objective To compare the changes of four flavonoid glycosides in the leaves of Hippophae rhamnoides L. before and after fermentation. Methods The water extract of Hippophae rhamnoides L. leaves and its fermented tea were concentrated and desiccated. The dry extracts were dissolved in 70% ethanol. The chromatographic separation was performed with RP-HPLC method on an Extend-C18 column (4.6 mm×250 mm, 5 μm). Acetonitrile-0.1% formic acid was selected as mobile phase at the flow rate of 1.0 ml/min. The detection wavelength was 356 nm and the column temperature was 30℃. Results The rutin content was high in the leaves of Hippophae rhamnoides L. After fermentation, isoquercitrin content was increased, while the contents of rutin and narcissoside were reduced and isorhamnetin-3-O-glucoside stayed unchanged. There was a good linear relationship between the concentration and peak areas of the four compounds (r>0.9997). The average recoveries were between 96%-103%. Conclusion This established method is rapid and reliable, which can be used for the quality control of Hippophae rhamnoides L. leaves and its fermented tea.

DONG Ruifang, WANG Zheng, CHEN Ling, SHU Zhiheng, HAN Ting, QIN Luping. Content variation of four flavonoid glycosides in Hippophae rhamnoides L. leaves before and after fermentation assayed by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 526-529. doi: 10.3969/j.issn.1006-0111.2017.06.011
Citation: DONG Ruifang, WANG Zheng, CHEN Ling, SHU Zhiheng, HAN Ting, QIN Luping. Content variation of four flavonoid glycosides in Hippophae rhamnoides L. leaves before and after fermentation assayed by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 526-529. doi: 10.3969/j.issn.1006-0111.2017.06.011
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