JIANG Yiping, XUE Liming, XIN Hailiang, ZHANG Qiaoyan, QIN Luping. Study on the technology for extracting lignans in schisandra chinensis (Turcz) Baill by AB-8 macroporous resin[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 520-525. doi: 10.3969/j.issn.1006-0111.2017.06.010
| Citation:
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JIANG Yiping, XUE Liming, XIN Hailiang, ZHANG Qiaoyan, QIN Luping. Study on the technology for extracting lignans in schisandra chinensis (Turcz) Baill by AB-8 macroporous resin[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 520-525. doi: 10.3969/j.issn.1006-0111.2017.06.010
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Study on the technology for extracting lignans in schisandra chinensis (Turcz) Baill by AB-8 macroporous resin
- 1.
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- 2.
Institute of Chemical Toxicity, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China
- Received Date: 2016-11-22
- Rev Recd Date:
2017-03-23
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Abstract
Objective To study the conditions for isolating and purifying lignans in schisandra chinensis (Turcz) Baill by AB-8 type of macroporous adsorption resin. Methods The content of Schizandrol, Deoxyschizandrin, r-Schisandrin was determined by HPLC. The optimum separation and purification process of lignans from schisandra chinensis (Turcz) Baill with AB-8 macroporous adsorption resin were identified by static and dynamic adsorption, desorption tests. The sample volume, concentration, eluting velocity, and ethanol concentration were investigated. Results The optimum conditions for isolating and purifying lignans in schisandra chinensis (Turcz) Baill by AB-8 macroporous adsorption resin were as follows:dosage of sample liquid was 1BV, concentration of sample solution was 9.159-16.523 mg/ml, ratio of diameter to height of resin column 1:5, adsoption flow rate was 2.5 BV/h, eluted by 5 BV 30% ethanol, followed by 10BV 95% ethanol. The transfer rate of lignans was 77.07% with 22.06% of total lignan content. Conclusion AB-8 macroporous adsorptive resin can effectively isolate and purify lignans from schisandra chinensis (Turcz) Baill. This low cost process is easy to operate and with high industrial production value.
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References
|
[1]
|
国家药典委员会. 中华人民共和国药典2015年版一部[S]. 北京:中国医药科技出版社, 2015:66-67. |
|
[2]
|
Cheng N, Ren NY, Gao H, et al. Antioxidant and hepatoprotective effects of Schisandra chinensis pollen extract on CCl4-induced acute liver damage in mice[J]. Food Chem Toxicol,2013, 55:234-240. |
|
[3]
|
陈泽慧, 田应彪, 李丹丹, 等. 五味子、石榴皮等中药对鲍曼不动杆菌体外的抑菌作用[J]. 中国医院药学杂志, 2011, 31(15):1248-1251. |
|
[4]
|
Kim EY, Baek IH, Rhyu MR. Cardioprotective effects of aqueous Schizandra chinensis fruit extract on ovariectomized and balloon-induced carotid artery injury rat models:effects on serum lipid profiles and blood pressure[J]. J Ethnopharmacol, 2011:134(3):668-75. |
|
[5]
|
Li LB, Zhang TR, Zhou Li, et al. Schisandrin B attenuates acetaminophen-induced hepatic injury through heat-shock protein 27 and 70 in mice[J]. J Gastroenterol Hepatol, 2014, 29(3):640-647. |
|
[6]
|
高雁, 李廷利. 五味子有效成分的药理作用研究进展[J]. 中医药学报, 2011, 39(6):104-106. |
|
[7]
|
孔华丽, 闫亮, 段惠娟. 五味子醇提取物保肝作用成分分析[J]. 解放军药学学报, 2010, 26(1):27-29. |
|
[8]
|
朱英, 何远, 李丽. 大孔树脂对地锦草、金银花叶与杭白菊总黄酮吸附分离性能研究[J]. 医学研究杂志, 2007, 36(1):56-59. |
|
[9]
|
贾永艳, 田效志, 李九席, 等. 丹参大孔树脂纯化工艺研究[J]. 河南科学, 2012, 30(4):427-430. |
|
[10]
|
蒋益萍, 张巧艳, 张宏, 等. 正交试验优选五味子木脂素类成分的提取工艺[J]. 中成药, 2013, 35(11):2390-2394. |
|
[11]
|
贾存勤, 李阳春, 屠鹏飞, 等. HPD系列大孔吸附树脂预处理方法研究[J]. 中国中药杂志, 2005, 30(18):1425-1427. |
|
[12]
|
孙振亮. 青葙子化学成分及保肝活性的研究[D]. 上海:第二军医大学, 2009. |
|
[13]
|
程振玉, 宋海燕, 杨英杰, 等. AB-8大孔吸附树脂静态纯化北五味子木脂素工艺研究[J]. 北方园艺, 2015(7):141-145. |
|
[14]
|
莫永俊, 汪春泉, 王琦, 等.大孔树脂纯化五味子总木脂素、总三萜工艺研究[J].中国医药导报, 2013, 10(5):106-108. |
|
[15]
|
黄家富, 周晓东, 周山.大孔吸附树脂纯化五味子木脂素的工艺考察[J].中国现代药物应用, 2014, 8(19):12-16. |
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