Determination of sinoporphyrin sodium in tumor-bearing mouse plasma by HPLC method

ZHU Bing; GAO Lihong; ZHAO Naping; LI Shanxin; ZHANG Li

doi:10.3969/j.issn.1006-0111.2017.06.007

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Objective To establish a HPLC method for the assay of sinoporphyrin sodium (DVDMS) in tumor-bearing mouse plasma and to study its pharmacokinetics. Methods The column was Waters XBridge C₁₈ (3.0 mm×100 mm, 3.5 μm). Gradient elution was applied with mobile phase A as the mixture of acetonitrile-methanol (20:80) and B as the aqueous solution of 1% acetic acid and 0.1% triethylamine at flow rate 0.7 ml/min. The detection wavelength was 380 nm. DVDMS was administrated to tumor-bearing mice by tail vein injection. The blood samples were collected at designated time and centrifuged for plasma. DVDMS in plasma samples were extracted by protein precipitation and analyzed by the HPLC method mentioned above. Pharmacokinetic parameters were calculated by DAS 2.0 with statistical moment analysis. Results DVDMS showed good linearity within the ranges of 70.8-14 160 ng/ml (r=0.9998). The main pharmacokinetic parameters were calculated as follows:c_max=(24 127.59±1 415.23) ng/ml, t_max=0.083 h, t_1/2=(9.59±1.25) h, MRT_0-∞=(11.77±1.73) h, AUC_0-∞=(34 775.83±6 185.43) h·ng/ml. Conclusion This HPLC method is sensitive, rapid and accurate, which can be used for analysis and research of DVDMS in plasma samples of tumor-bearing mice.

Determination of sinoporphyrin sodium in tumor-bearing mouse plasma by HPLC method

Department of Pharmacy, Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433, China

Received Date: 2017-07-14

Rev Recd Date: 2017-09-28

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Abstract: Objective To establish a HPLC method for the assay of sinoporphyrin sodium (DVDMS) in tumor-bearing mouse plasma and to study its pharmacokinetics. Methods The column was Waters XBridge C₁₈ (3.0 mm×100 mm, 3.5 μm). Gradient elution was applied with mobile phase A as the mixture of acetonitrile-methanol (20:80) and B as the aqueous solution of 1% acetic acid and 0.1% triethylamine at flow rate 0.7 ml/min. The detection wavelength was 380 nm. DVDMS was administrated to tumor-bearing mice by tail vein injection. The blood samples were collected at designated time and centrifuged for plasma. DVDMS in plasma samples were extracted by protein precipitation and analyzed by the HPLC method mentioned above. Pharmacokinetic parameters were calculated by DAS 2.0 with statistical moment analysis. Results DVDMS showed good linearity within the ranges of 70.8-14 160 ng/ml (r=0.9998). The main pharmacokinetic parameters were calculated as follows:c_max=(24 127.59±1 415.23) ng/ml, t_max=0.083 h, t_1/2=(9.59±1.25) h, MRT_0-∞=(11.77±1.73) h, AUC_0-∞=(34 775.83±6 185.43) h·ng/ml. Conclusion This HPLC method is sensitive, rapid and accurate, which can be used for analysis and research of DVDMS in plasma samples of tumor-bearing mice.

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Determination of sinoporphyrin sodium in tumor-bearing mouse plasma by HPLC method

doi: 10.3969/j.issn.1006-0111.2017.06.007

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