Determination of total anthraquinones in Xiaofan lotion by HPLC method

HUANG Juan; ZHANG Qinglian; PI Fengjuan; ZHANG Shibo; ZHAO Ling; LIU Hao

doi:10.3969/j.issn.1006-0111.2017.05.013

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Objective To develop a method for the determination of total anthraquinones in Xiaofan lotion by HPLC. Methods Rhubarb anthraquinones were simultaneously separated and assayed on a Kromasil C₁₈ column(250 mm×4.6 mm,5 μm) with the mobile phase of 0.1%phosphoric acid-methanol (15:85) at 30 ℃.The detection wavelength was 207 nm. Results The linear relationship is good for Aloe-emodin and other five standard ingredients. The average recovery was 99.45%, RSD 1.68%. Conclusion The method is simple, rapid and accurate. It is suitable for determination of total anthraquinones in Xiaofan lotion.

Determination of total anthraquinones in Xiaofan lotion by HPLC method

1. Department of Pharmacy, Luzhou TCM Hospital, Luzhou 646000, China

2. Department of Pharmaceutics, School of Pharmacy, Southwest Medical University, Luzhou 646000, China

Received Date: 2017-02-06

Rev Recd Date: 2017-05-31

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Abstract: Objective To develop a method for the determination of total anthraquinones in Xiaofan lotion by HPLC. Methods Rhubarb anthraquinones were simultaneously separated and assayed on a Kromasil C₁₈ column(250 mm×4.6 mm,5 μm) with the mobile phase of 0.1%phosphoric acid-methanol (15:85) at 30 ℃.The detection wavelength was 207 nm. Results The linear relationship is good for Aloe-emodin and other five standard ingredients. The average recovery was 99.45%, RSD 1.68%. Conclusion The method is simple, rapid and accurate. It is suitable for determination of total anthraquinones in Xiaofan lotion.

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Determination of total anthraquinones in Xiaofan lotion by HPLC method

doi: 10.3969/j.issn.1006-0111.2017.05.013

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