Determination of seven components in Pifubingxuedu tablet by HPLC method

SHI Zhihui; XIAO Huimin; HE Yue; LIU Yang; LIU Haiyue; YANG Xu; WANG Siwang

doi:10.3969/j.issn.1006-0111.2017.05.010

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Objective To establish a HPLC method for simultaneous determination of seven constituents in Pifubingxuedu tablet. Methods The determination was performed on Kromasil-C₁₈ column (4.6 mm×250 mm, 5 μm).The mobile phase was acetonitrile-0.5% phosphoric acid aqueous solution with a flow rate of 0.8ml/min. The detector was PDA and detection wavelength was set at 245,325,403 nm, respectively. Results A method has been established for the determination of chlorogenic acid, hydroxy Safflower Pigment A, paeoniflorin, forsythiaside A, ferulic acid, berberine and glycyrrhizin acid in one run by HPLC. Their average contents and RSD in each Pifubingxuedu tablet were 0.299 5 mg/tablet (2.25%);0.700 0 mg/tablet(1.33%);0.429 2 mg/tablet (1.21%);0.039 1 mg/tablet (2.34%);0.014 8 mg/tablet(2.23%);0.209 0 mg/tablet (2.06%);0.272 7 mg/tablet (2.68%), respectively. Conclusion The established method is simple, convenient, accurate and reliable. It is suitable for the quality control of Pifubingxuedu tablet.

Determination of seven components in Pifubingxuedu tablet by HPLC method

1. Shaanxi Jun Bi Sha Pharmaceutical Limited Company, Xianyang 712000, China

2. New Materia Medica Research and Development Center, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China

3. Shaanxi University of Chinese Medicine, Xianyang 712046, China

Received Date: 2016-09-05

Rev Recd Date: 2016-12-27

Key words:

Abstract: Objective To establish a HPLC method for simultaneous determination of seven constituents in Pifubingxuedu tablet. Methods The determination was performed on Kromasil-C₁₈ column (4.6 mm×250 mm, 5 μm).The mobile phase was acetonitrile-0.5% phosphoric acid aqueous solution with a flow rate of 0.8ml/min. The detector was PDA and detection wavelength was set at 245,325,403 nm, respectively. Results A method has been established for the determination of chlorogenic acid, hydroxy Safflower Pigment A, paeoniflorin, forsythiaside A, ferulic acid, berberine and glycyrrhizin acid in one run by HPLC. Their average contents and RSD in each Pifubingxuedu tablet were 0.299 5 mg/tablet (2.25%);0.700 0 mg/tablet(1.33%);0.429 2 mg/tablet (1.21%);0.039 1 mg/tablet (2.34%);0.014 8 mg/tablet(2.23%);0.209 0 mg/tablet (2.06%);0.272 7 mg/tablet (2.68%), respectively. Conclusion The established method is simple, convenient, accurate and reliable. It is suitable for the quality control of Pifubingxuedu tablet.

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[1]	国家药典委员会.中华人民共和国药典2015年版一部［S］.北京:中国医药科技出版社,2015:221,193, 221,193,151,158,105,41,133,171,305,87.
[2]	魏明,杨晓梅,刘佳红,等. 绿原酸的药理作用研究进展［J］. 陕西中医, 2016, 37 (4): 511-512.
[3]	扈晓佳,殷莎,袁婷婷,等. 红花的化学成分及其药理活性研究进展［J］. 药学实践杂志,2013,31(3): 161-169.
[4]	郑世存,李晓宇,欧阳兵,等. 芍药苷药理作用研究新进展［J］. 中国药物警戒,2012, 9(2):100-103.
[5]	肖会敏,王四旺,王剑波,等.连翘抗病毒的研究进展［J］.中国医药导报,2010,7(2):9-10.
[6]	梁娜,孙少平,罗跃娥,等. 阿魏酸的研究进展［J］.黑龙江中医药,2009,7(2):39-40.
[7]	陈阳峰,钟晓红. 黄柏的药理作用及其活性成分提取［J］.作物研究, 2015, 29 (5): 564-568.
[8]	吕春霞,冯玉红.甘草临床应用概况［J］. 内蒙古中医药,2003,22(S1):42-43.
[9]	王吉锡,孟丹,张玉瑶,等.甘草抗流感病毒作用的研究探讨［J］. 中医药信息, 2010, 27 (1): 42-43.
[10]	刘育辰,陈有根,王丹,等. 甘草化学成分研究［J］.药物分析杂志, 2011,31(7): 1251-1255.
[11]	肖会敏,何悦,王四旺,等.四季抗病毒合剂的超快速液相色谱法指纹图谱研究及4个成分含量测定［J］.中国医院药学杂志, 2015,35(3): 230-233.

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Determination of seven components in Pifubingxuedu tablet by HPLC method

doi: 10.3969/j.issn.1006-0111.2017.05.010

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