WANG Kebing, CHEN Zhihong, GUO Bu, LU Guozhong. Rapid identification of inactivated C. albicans by surface-enhanced Raman spectroscopy[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(5): 422-426. doi: 10.3969/j.issn.1006-0111.2017.05.009
| Citation:
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WANG Kebing, CHEN Zhihong, GUO Bu, LU Guozhong. Rapid identification of inactivated C. albicans by surface-enhanced Raman spectroscopy[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(5): 422-426. doi: 10.3969/j.issn.1006-0111.2017.05.009
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Rapid identification of inactivated C. albicans by surface-enhanced Raman spectroscopy
- Received Date: 2017-05-10
- Rev Recd Date:
2017-07-06
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Abstract
Objective To develop a rapid method for the identification of inactivated C. albicans by surface-enhanced Raman spectroscopy (SERS). Methods Live C. albicans cultures were exposed to heating, formaldehyde and fungicidal drug (amphotericin B). The corresponding SERS spectra were acquired for the investigation and comparison. Results The spectra acquired with three different inactivation methods exhibited similar features of dead C. albicans, which showed significant difference from the spectra of the live culture. Conclusion This SERS method can identify the inactivated C. albicans rapidly. Hopefully it will provide a convenient tool for quick identification of other inactivated pathogenic microorganisms.
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