Berberine hydrochloride assay in Dongbai Tonglin Heji by HPLC method

HOU Xiaoli; TAN Zhaodan; SHI Kourong; GU Weiying; CHEN Weicheng

doi:10.3969/j.issn.1006-0111.2017.04.016

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Objective To establish a HPLC method for determination of berberine hydrochloride in Dongbai Tonglin Heji. Methods The separation column of Kromasil C₁₈(150 mm×4.6 mm,5 μm) was used. The mobile phase was acetonitrile and 0.05 mol/L NaH₂PO₄solution(pH was adjusted to about 3 with phosphoric acid). The flow rate was 1.0 ml/min with the column at room temperature and the detection wavelengths at 345 nm for berberine hydrochloride. Results The linear range of berberine hydrochloride was 1.00~50.00 μg/ml(r=0.999 6). The average recoveries of berberine hydrochloride were 104.70%(RSD 1.60%, n=5). Conclusion This method is accurate, sensitive, selective and reproducible. It provides an alternative method to improve the quality control of Dongbai Tonglin Heji.

Berberine hydrochloride assay in Dongbai Tonglin Heji by HPLC method

Department of Pharmacy, Seventh People's Hospital Affiliated to Shanghai University of TCM, 200137, China

Received Date: 2017-03-02

Rev Recd Date: 2017-05-31

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Abstract: Objective To establish a HPLC method for determination of berberine hydrochloride in Dongbai Tonglin Heji. Methods The separation column of Kromasil C₁₈(150 mm×4.6 mm,5 μm) was used. The mobile phase was acetonitrile and 0.05 mol/L NaH₂PO₄solution(pH was adjusted to about 3 with phosphoric acid). The flow rate was 1.0 ml/min with the column at room temperature and the detection wavelengths at 345 nm for berberine hydrochloride. Results The linear range of berberine hydrochloride was 1.00~50.00 μg/ml(r=0.999 6). The average recoveries of berberine hydrochloride were 104.70%(RSD 1.60%, n=5). Conclusion This method is accurate, sensitive, selective and reproducible. It provides an alternative method to improve the quality control of Dongbai Tonglin Heji.

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Berberine hydrochloride assay in Dongbai Tonglin Heji by HPLC method

doi: 10.3969/j.issn.1006-0111.2017.04.016

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