Research on quality standard of Santeng oral solution

CHEN Xiaoling; CHEN Shun; GAO Shouhong; SHU Chang; PANG Tao; Lu Wenquan

doi:10.3969/j.issn.1006-0111.2017.04.015

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Objective To establish the quality standard of Santeng oral solution. Method Sargentodoxa Caulis and Spatholobi Caulis were identified by thin layer chromatography. Chlorogenic acid was assayed by high performance liquid chromatography. The chromatographic column is Agilent Zorbax SB C₁₈ (4.6 mm×250 mm, 5 μm) with a stable temperature of 35 ℃. The mobile phase in isocratic elution consists of acetonitrile and 0.1% folic acid aqueous solution with a preliminary volume ratio of 9∶91. The flow rate is 1.0 ml/min with an injection volume of 20 μl. Results Thin layer chromatography showed distinct spots of Sargentodoxa Caulis and Spatholobi Caulis with a great specificity. A regression formula Y=60.14X－6.37(r>0.999 9) was obtained with a good linearity in concentration range of 2.70~202.50 μg/ml. Conclusion A simple, stable and repeatable method was established for the quality control of Santeng oral solution.

Research on quality standard of Santeng oral solution

Department of Pharmacy, Changzheng Hospital Affiliated to Second Military Medical University, Shanghai 200003, China

Received Date: 2017-02-28

Rev Recd Date: 2017-04-24

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Abstract: Objective To establish the quality standard of Santeng oral solution. Method Sargentodoxa Caulis and Spatholobi Caulis were identified by thin layer chromatography. Chlorogenic acid was assayed by high performance liquid chromatography. The chromatographic column is Agilent Zorbax SB C₁₈ (4.6 mm×250 mm, 5 μm) with a stable temperature of 35 ℃. The mobile phase in isocratic elution consists of acetonitrile and 0.1% folic acid aqueous solution with a preliminary volume ratio of 9∶91. The flow rate is 1.0 ml/min with an injection volume of 20 μl. Results Thin layer chromatography showed distinct spots of Sargentodoxa Caulis and Spatholobi Caulis with a great specificity. A regression formula Y=60.14X－6.37(r>0.999 9) was obtained with a good linearity in concentration range of 2.70~202.50 μg/ml. Conclusion A simple, stable and repeatable method was established for the quality control of Santeng oral solution.

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Reference (8)

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Research on quality standard of Santeng oral solution

doi: 10.3969/j.issn.1006-0111.2017.04.015

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