LIU Man, LIU Liyun, SUN Fan, LIN Houwen. The discovery of antitumor compounds from marine sponge targeting lung cancer stem cells[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(4): 304-307. doi: 10.3969/j.issn.1006-0111.2017.04.005
| Citation:
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LIU Man, LIU Liyun, SUN Fan, LIN Houwen. The discovery of antitumor compounds from marine sponge targeting lung cancer stem cells[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(4): 304-307. doi: 10.3969/j.issn.1006-0111.2017.04.005
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The discovery of antitumor compounds from marine sponge targeting lung cancer stem cells
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Department of Pharmacy, Renji Hospital Affiliated to School of Medicine, Shanghai JiaoTong University, Shanghai 200127, China
- Received Date: 2017-03-28
- Rev Recd Date:
2017-05-10
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Abstract
Objective To screen the active compounds targeting lung cancer stem cells (LCSCs) from the sponge Aaptos aaptos. Methods The A549-Nanog-GFP model of LCSCs was constructed. Western blot and immunofluorescence were used to examine the expression of pluripotency markers in screening model. The established LCSCs model was used to screen 8 fractions of Aaptos aaptos dichloromethane extract. The active fraction was separated by various chromatographic methods. CCK8 assay was used to screen the compounds for anti-LCSCs activity in vitro. Results LCSCs model with high expression of CD44 and ALDH1A1 protein was successfully constructed. The fraction D6 showed significant inhibitory activity in LCSCs(P<0.01). Four aaptamine alkaloids were isolated from this fraction. Compound AP-1 has good activity against LCSCs(P<0.01)with IC50 value(3.84±0.12) μmol/L. Conclusion AP-1 isolated from the sponge Aaptos aaptos exhibited significant activity against LCSCs.
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