The methodology validation and content assay of total polysaccharides in Pudi Enema

WANG Yihong; CHEN Jinshan; XU Caiyan; ZHENG Shaozhong

doi:10.3969/j.issn.1006-0111.2017.03.013

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Objective To establish the assay method for the total polysaccharide in Pudi Enema. Methods Phenol-sulfuric acid method was used for chromogenic reaction. The content of total polysaccharide was measured by UV spectrophotometry at 488.8 nm. Results The total polysaccharides calibration curve was at the range of 0~22.635 mg/L, with regression function being Y=0.062 06 X-0.003 34(r=0.999 8). The recovery of calycosin was 98.36%(RSD=2.34%). Conclusion This method is sensitive,rapid,accurate and reliable. It can be used to assay the content of total polysaccharide in Pudi Enema.

The methodology validation and content assay of total polysaccharides in Pudi Enema

1. No. 175 Hospital of PLA, the Affiliated Southeast Hospital of Xiamen University, Compounding Pharmacy, Zhangzhou 363000, China

2. No. 175 Hospital of PLA, the Affiliated Southeast Hospital of Xiamen University, Central Pharmacy, Zhangzhou 363000, China

Received Date: 2016-02-17

Rev Recd Date: 2016-07-19

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Abstract: Objective To establish the assay method for the total polysaccharide in Pudi Enema. Methods Phenol-sulfuric acid method was used for chromogenic reaction. The content of total polysaccharide was measured by UV spectrophotometry at 488.8 nm. Results The total polysaccharides calibration curve was at the range of 0~22.635 mg/L, with regression function being Y=0.062 06 X-0.003 34(r=0.999 8). The recovery of calycosin was 98.36%(RSD=2.34%). Conclusion This method is sensitive,rapid,accurate and reliable. It can be used to assay the content of total polysaccharide in Pudi Enema.

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The methodology validation and content assay of total polysaccharides in Pudi Enema

doi: 10.3969/j.issn.1006-0111.2017.03.013

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