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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2017  >  35(2): 130-133

CHEN Zhe, LU Juan, PENG Jiming, CHAI Ruiping, CHEN Xi. Simultaneous determination of 14 amino acids in Pfaffia by RP-HPLC with precolumn derivatization[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(2): 130-133. doi: 10.3969/j.issn.1006-0111.2017.02.008
Citation: CHEN Zhe, LU Juan, PENG Jiming, CHAI Ruiping, CHEN Xi. Simultaneous determination of 14 amino acids in Pfaffia by RP-HPLC with precolumn derivatization[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(2): 130-133. doi: 10.3969/j.issn.1006-0111.2017.02.008
shu

Simultaneous determination of 14 amino acids in Pfaffia by RP-HPLC with precolumn derivatization

doi: 10.3969/j.issn.1006-0111.2017.02.008
  • 1.

    Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China

  • 2.

    Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;Shandong University of Chinese Medicine, Jinan 250355, China

  • Received Date: 2016-10-13
  • Rev Recd Date: 2016-12-16
  • Objective To develop a HPLC method for determining 14 hydrolyzed amino acids in Pfaffia. Methods The sample was derivatized with phenyl isothiocyanate (PITC). Amino acids were separated on Waters XBridge Shield RP18 (4.6 mm×250 mm, 5 μm) column at the flow rate of 0.8 ml/min, detected at 254 nm. The column temperature was 25℃. Results The response was linear for 14 amino acids with a correlation coefficient r>0.999 0. The average recoveries (n=6) were 90.2%-105.1%. Amino acids derivative solution remained stable in 24 hours. Conclusion This well-established method is very reliable. It can be used as a quantitative determination method for 14 amino acids in Pfaffia.
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    [10] Takemoto T,Nishimoto N,Nakai S,et al. Pfaffic acid, a novel nortriterpene from Pfaffia paniculata Kuntze[J].Tetrahedr lett,1983,24(10):1057-1060.
    [11] 李海生,李华龙.用于氨基酸分析供试品的水解和处理方法[J].天津药学,1999,11(3):54-55.
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Simultaneous determination of 14 amino acids in Pfaffia by RP-HPLC with precolumn derivatization

doi: 10.3969/j.issn.1006-0111.2017.02.008
  • 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China
  • 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;Shandong University of Chinese Medicine, Jinan 250355, China
  • Received Date: 2016-10-13
  • Rev Recd Date: 2016-12-16

Abstract: Objective To develop a HPLC method for determining 14 hydrolyzed amino acids in Pfaffia. Methods The sample was derivatized with phenyl isothiocyanate (PITC). Amino acids were separated on Waters XBridge Shield RP18 (4.6 mm×250 mm, 5 μm) column at the flow rate of 0.8 ml/min, detected at 254 nm. The column temperature was 25℃. Results The response was linear for 14 amino acids with a correlation coefficient r>0.999 0. The average recoveries (n=6) were 90.2%-105.1%. Amino acids derivative solution remained stable in 24 hours. Conclusion This well-established method is very reliable. It can be used as a quantitative determination method for 14 amino acids in Pfaffia.

CHEN Zhe, LU Juan, PENG Jiming, CHAI Ruiping, CHEN Xi. Simultaneous determination of 14 amino acids in Pfaffia by RP-HPLC with precolumn derivatization[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(2): 130-133. doi: 10.3969/j.issn.1006-0111.2017.02.008
Citation: CHEN Zhe, LU Juan, PENG Jiming, CHAI Ruiping, CHEN Xi. Simultaneous determination of 14 amino acids in Pfaffia by RP-HPLC with precolumn derivatization[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(2): 130-133. doi: 10.3969/j.issn.1006-0111.2017.02.008
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