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Volume 34 Issue 2
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2016  >  34(2): 174-176,187

LIN Hongwei, PIAO Shujuan, JIANG Chunxia, LI Jingxian, LU Wenquan, CHEN Wansheng. The fingerprint and durability study on various regions of Polygonum cuspidatum[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(2): 174-176,187. doi: 10.3969/j.issn.1006-0111.2016.02.019
Citation: LIN Hongwei, PIAO Shujuan, JIANG Chunxia, LI Jingxian, LU Wenquan, CHEN Wansheng. The fingerprint and durability study on various regions of Polygonum cuspidatum[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(2): 174-176,187. doi: 10.3969/j.issn.1006-0111.2016.02.019
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The fingerprint and durability study on various regions of Polygonum cuspidatum

doi: 10.3969/j.issn.1006-0111.2016.02.019

  • Department of Pharmacy, Changzheng Hospital Affiliated to Second Military Medical University, Shanghai 200003, China

  • Received Date: 2014-11-28
  • Rev Recd Date: 2015-05-18
  • Objective To study the fingerprint of Polygonum cuspidatum from various regions by HPLC and provide technical support for the identification. Methods HPLC conditions:the chromatographic column was Agilent ZORBAX SB-C18(4.6 mm×250 mm, 5μm); the mobile phase was 0.1% formic acid in water and acetonitrile(A-B); gradient elution; the detection wavelength was 306 nm; the flow rate was 1 ml/min; the column temperature was 30℃; injection volume was 20μl. Results The HPLC gradient conditions could effectively separate polydatin, resveratrol, emodin and emodin ether, etc. Conclusion This method is stable, effective and durable, which is instructive to the herbs data identification of Polygonum cuspidatum.
  • [1] 国家药典委员会.中华人民共和国药典2010年版一部[S]. 北京:中国医药科技出版社,2010:194.
    [2] 江苏新医学院. 中药大词典[M]. 上海:上海科学技术出版社,1997:1329-1331.
    [3] 肖凯,宣利江,徐亚明,等. 虎杖的化学成分研究[J]. 中药及天然药物,2003,38(1):496-498.
    [4] 黄拓,江海燕,卢忠朋,等. 虎杖炮制的历史沿革及其饮片质量研究概况[J]. 中医药学刊,2005,23(9):1611.
    [5] 笔雪艳,胡畔,张胜波,等.用超高效液相色谱蒸发光散射检测桔梗流浸膏指纹图谱的研究[J].药学实践杂志,2015,33(2):153-155.
    [6] 张明昶,彭小冰,苗旭辉,等. 中药虎杖特征指纹图谱研究[J]. 中国实验方剂学杂志,2011,17(1):53-55.
    [7] 成守玲,廖彩震,郑太华,等. 虎杖药材的高效液相指纹图谱研究[J]. 世界中西医结合杂志,2014,9(2):165-168.
    [8] 李淼,楼一层,杨翰,等. 复方虎杖高效液相色谱指纹图谱的研究[J]. 武汉植物学研究,2009,27(4):446-450.
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The fingerprint and durability study on various regions of Polygonum cuspidatum

doi: 10.3969/j.issn.1006-0111.2016.02.019
  • Department of Pharmacy, Changzheng Hospital Affiliated to Second Military Medical University, Shanghai 200003, China
  • Received Date: 2014-11-28
  • Rev Recd Date: 2015-05-18

Abstract: Objective To study the fingerprint of Polygonum cuspidatum from various regions by HPLC and provide technical support for the identification. Methods HPLC conditions:the chromatographic column was Agilent ZORBAX SB-C18(4.6 mm×250 mm, 5μm); the mobile phase was 0.1% formic acid in water and acetonitrile(A-B); gradient elution; the detection wavelength was 306 nm; the flow rate was 1 ml/min; the column temperature was 30℃; injection volume was 20μl. Results The HPLC gradient conditions could effectively separate polydatin, resveratrol, emodin and emodin ether, etc. Conclusion This method is stable, effective and durable, which is instructive to the herbs data identification of Polygonum cuspidatum.

LIN Hongwei, PIAO Shujuan, JIANG Chunxia, LI Jingxian, LU Wenquan, CHEN Wansheng. The fingerprint and durability study on various regions of Polygonum cuspidatum[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(2): 174-176,187. doi: 10.3969/j.issn.1006-0111.2016.02.019
Citation: LIN Hongwei, PIAO Shujuan, JIANG Chunxia, LI Jingxian, LU Wenquan, CHEN Wansheng. The fingerprint and durability study on various regions of Polygonum cuspidatum[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(2): 174-176,187. doi: 10.3969/j.issn.1006-0111.2016.02.019
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